Alsever's solution

Alsever's solution is a saline liquid used to prevent coagulation of blood.[1][2] It is composed of 2.05% dextrose, 0.8% sodium citrate, 0.055% citric acid, and 0.42% sodium chloride. For usage, an equal volume of blood is gently, but thoroughly, mixed with the solution. This solution is used to study in vitro anti-inflammatory activity of crude drugs by the human red blood cell stabilization method.[3] It is also used to preserve blood cells from other sources.[4][5]

The test was invented in 1941 by the American Hematologist, John Bellows Alsever (1908–1978).

References

  1. ↑ Alsever, J. B., & Ainslie, R. B. (1941). A new method for the preparation of dilute blood plasma and the operation of a complete transfusion service. NY State J. Med, 41, 126-131.
  2. ↑ Campbell, D. H.; Garvey, J. S.; Cremer, N. E.; Sussdorf, D. H. (1963). Methods in Immunology. A Laboratory Text for Instruction and Research. New York: W. A. Benjamin, Inc. p. 244.
  3. ↑ Gandhisan R, Thamaraichelvan A, Baburaj. Antiinflammatory action of Lannea coromandelica HRBC membrane stabilization. Fitotherapia 1991; 62: 82-83.
  4. ↑ Schjerning-Thiesen, Knud (2009). "Experiments on the Stability of Sheep Erythrocytes Stored in Alsever's Solution". Acta Pathologica et Microbiologica Scandinavica. 32 (1): 198–203. doi:10.1111/j.1699-0463.1953.tb00242.x. PMID 13050415.
  5. ↑ L.S.A., List of C.F.R. Sections Affected. 2003. p. 827.
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