2,5-diketocamphane 1,2-monooxygenase

2,5-diketocamphane 1,2-monooxygenase (EC 1.14.14.108, 2,5-diketocamphane lactonizing enzyme, ketolactonase I, 2,5-diketocamphane 1,2-monooxygenase oxygenating component, 2,5-DKCMO, camphor 1,2-monooxygenase, camphor ketolactonase I) is an enzyme with systematic name (+)-bornane-2,5-dione,NADH:oxygen oxidoreductase (1,2-lactonizing).[1][2][3][4][5][6] This enzyme catalyses the following chemical reaction

(+)-bornane-2,5-dione + O2 + NADH + H+ (+)-5-oxo-1,2-campholide + NAD+ + H2O
2,5-diketocamphane 1,2-monooxygenase
Identifiers
EC no.1.14.14.108
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Search
PMCarticles
PubMedarticles
NCBIproteins

2,5-diketocamphane 1,2-monooxygenase is a flavoprotein (FMN) which requires Fe2+.

References
  1. Conrad HE, Dubus R, Namtvedt MJ, Gunsalus IC (January 1965). "Mixed function oxidation. II. Separation and properties of the enzymes catalyzing camphor lactonizaton". The Journal of Biological Chemistry. 240: 495–503. PMID 14253460.
  2. Yu CA, Gunsalus IC (November 1969). "Monoxygenases. VII. Camphor ketolactonase I and the role of three protein components". The Journal of Biological Chemistry. 244 (22): 6149–52. PMID 4310834.
  3. Taylor DG, Trudgill PW (February 1986). "Camphor revisited: studies of 2,5-diketocamphane 1,2-monooxygenase from Pseudomonas putida ATCC 17453". Journal of Bacteriology. 165 (2): 489–97. PMC 214445. PMID 3944058.
  4. Selifonov SA (August 1992). "Microbial oxidation of adamantanone by Pseudomonas putida carrying the camphor catabolic plasmid". Biochemical and Biophysical Research Communications. 186 (3): 1429–36. doi:10.1016/s0006-291x(05)81566-9. PMID 1510672.
  5. Jones KH, Smith RT, Trudgill PW (April 1993). "Diketocamphane enantiomer-specific 'Baeyer-Villiger' monooxygenases from camphor-grown Pseudomonas putida ATCC 17453". Journal of General Microbiology. 139 (4): 797–805. doi:10.1099/00221287-139-4-797. PMID 8515237.
  6. Kadow M, Saß S, Schmidt M, Bornscheuer UT (June 2011). "Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007". AMB Express. 1 (1): 13. doi:10.1186/2191-0855-1-13. PMC 3222318. PMID 21906366.
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