6-methylsalicylic-acid synthase

In enzymology, a 6-methylsalicylic-acid synthase (EC 2.3.1.165) is a polyketide synthase that catalyzes the chemical reaction

acetyl-CoA + 3 malonyl-CoA + NADPH + H+ Failed to parse (SVG (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "http://localhost:6011/en.wikipedia.org/v1/":): \rightleftharpoons 6-methylsalicylate + 4 CoA + 3 CO2 + NADP+ + H2O
6-methylsalicylic acid synthase
Identifiers
EC no.2.3.1.165
CAS no.9045-37-8
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
Search
PMCarticles
PubMedarticles
NCBIproteins

6-methylsalicylic-acid synthase reaction

The 4 substrates of this enzyme are acetyl-CoA, malonyl-CoA, NADPH, and H+, whereas its 5 products are 6-methylsalicylate, CoA, CO2, NADP+, and H2O.

This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. The systematic name of this enzyme class is acyl-CoA:malonyl-CoA C-acyltransferase (decarboxylating, oxoacyl-reducing, thioester-hydrolysing and cyclizing). Other names in common use include MSAS, and 6-methylsalicylic acid synthase.

References

    • Spencer JB, Jordan PM (December 1992). "Purification and properties of 6-methylsalicylic acid synthase from Penicillium patulum". Biochem. J. 288 (Pt 3): 839–46. PMC 1131963. PMID 1471999.
    • Child CJ, Spencer JB, Bhogal P, Shoolingin-Jordan PM (1996). "Structural similarities between 6-methylsalicylic acid synthase from Penicillium patulum and vertebrate type I fatty acid synthase: evidence from thiol modification studies". Biochemistry. 35 (38): 12267–74. doi:10.1021/bi960422e. PMID 8823160.
    • Richardson MT, Pohl NL, Kealey JT, Khosla C (1999). "Tolerance and specificity of recombinant 6-methylsalicyclic acid synthase". Metab. Eng. 1 (2): 180–7. doi:10.1006/mben.1999.0113. PMID 10935930.


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