Adenosylhomocysteinase

Adenosylhomocysteinase (EC 3.3.1.1, S-adenosylhomocysteine synthase, S-adenosylhomocysteine hydrolase, adenosylhomocysteine hydrolase, S-adenosylhomocysteinase, SAHase, AdoHcyase) is an enzyme that converts S-adenosylhomocysteine to homocysteine and adenosine.[1][2] This enzyme catalyses the following chemical reaction

S-adenosyl-L-homocysteine + H2O ⇌ L-homocysteine + adenosine
S-Adenosylhomocysteine hydrolase
SAH hydrolase tetramer, Human
Identifiers
SymbolAHCY
NCBI gene191
HGNC343
OMIM180960
RefSeqNM_000687
UniProtP23526
Other data
EC number3.3.1.1
LocusChr. 20 q11.22
Search for
StructuresSwiss-model
DomainsInterPro

The enzyme contains one tightly bound NAD+ per subunit. The mechanism involves dehydrogenative oxidation of the 3'-OH of the ribose. The resulting ketone is susceptible to α-deprotonation. The resulting carbanion eliminates thiolate. The a,b-unsaturated ketone is then hydrated, and the ketone is reduced by the NADH.

This enzyme is encoded by the AHCY gene in humans,[3][4] which is believed to have a prognostic role in neuroblastoma.[5]

References
  1. De La Haba G, Cantoni GL (March 1959). "The enzymatic synthesis of S-adenosyl-L-homocysteine from adenosine and homocysteine". The Journal of Biological Chemistry. 234 (3): 603–8. doi:10.1016/S0021-9258(18)70253-6. PMID 13641268.
  2. Palmer JL, Abeles RH (February 1979). "The mechanism of action of S-adenosylhomocysteinase". The Journal of Biological Chemistry. 254 (4): 1217–26. doi:10.1016/S0021-9258(17)34190-X. PMID 762125.
  3. GeneCards.org - AHCY Gene - Adenosylhomocysteinase
  4. NLM - AHCY adenosylhomocysteinase
  5. Chicco, Davide; Sanavia, Tiziana; Jurman, Giuseppe (4 March 2023). "Signature literature review reveals AHCY, DPYSL3, and NME1 as the most recurrent prognostic genes for neuroblastoma". BioData Mining. 16 (1). doi:10.1186/s13040-023-00325-1. eISSN 1756-0381. PMC 9985261. PMID 36870971.

Further reading
This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.