Aminoglycoside N6'-acetyltransferase

In enzymology, an aminoglycoside N6'-acetyltransferase (EC 2.3.1.82) is an enzyme that catalyzes the chemical reaction

acetyl-CoA + kanamycin-B CoA + N6'-acetylkanamycin-B
aminoglycoside N6'-acetyltransferase
Identifiers
EC no.2.3.1.82
CAS no.56467-65-3
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
Search
PMCarticles
PubMedarticles
NCBIproteins

Thus, the two substrates of this enzyme are acetyl-CoA and kanamycin B, whereas its two products are CoA and N6'-acetylkanamycin-B.

This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. The systematic name of this enzyme class is acetyl-CoA:kanamycin-B N6'-acetyltransferase. Other names in common use include aminoglycoside 6'-N-acetyltransferase, aminoglycoside-6'-acetyltransferase, aminoglycoside-6-N-acetyltransferase, and kanamycin acetyltransferase.

Structural studies

As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes 1S3Z, 1S5K, 1S60, and 2A4N.

References

    • Le Goffic F, Martel A (1974). "[Resistance to aminosides induced by an isoenzyme, kanamycin acetyltransferase]". Biochimie. 56 (6–7): 893–7. doi:10.1016/s0300-9084(74)80512-2. PMID 4614862.
    • Benveniste R, Davies J (1971). "Enzymatic acetylation of aminoglycoside antibiotics by Escherichia coli carrying an R factor". Biochemistry. 10 (10): 1787–96. doi:10.1021/bi00786a009. PMID 4935296.
    • Dowding JE (1977). "Mechanisms of gentamicin resistance in Staphylococcus aureus". Antimicrob. Agents Chemother. 11 (1): 47–50. doi:10.1128/aac.11.1.47. PMC 351917. PMID 836013.


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