Aniline Blue WS

Aniline Blue WS, also called aniline blue, diphenylamine blue, China blue, or Soluble blue, is a mixture of methyl blue and water blue. It may also be either one of them.[1] It is a soluble dye used as a biological dye,[2] in fluorescence microscopy, appearing a yellow-green colour after excitation with violet light.[3] It is a mixture of the trisulfonates of triphenyl rosaniline and of diphenyl rosaniline.[4]

Water blue
Methyl blue

Aniline blue or its constituents are used to stain collagen, as the fibre stain in Masson's trichrome,[5] as well as to reveal callose structures in plant tissues.[6]

It can also be used in other connective tissue stains, such as Mallory's stain,[5] Gömöri trichrome stain, and Carstair's Method.[7] It is used in differential staining.

References
  1. "Stainsfile - Aniline blue WS". stainsfile.info. Archived from the original on 2019-02-09. Retrieved 2012-01-14.
  2. "Medical Definition of ANILINE BLUE". www.merriam-webster.com.
  3. "Fluorescence Microscope Images". Archived from the original on 2008-05-26. Retrieved 2012-01-14.
  4. "aniline blue".
  5. "Stainsfile - Water blue". stainsfile.info. Archived from the original on 2012-02-07. Retrieved 2012-01-14.
  6. "Protocols - Staining with trypan blue and aniline blue - Felix Mauch's Group". Archived from the original on 22 June 2005.
  7. Carstairs, K. C. (1965). "The Identification of platelets and platelet antigens in histological sections". The Journal of Pathology and Bacteriology. 90 (1): 225-231.
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