Antibody barcoding

Antibody barcoding is a protein profiling technique that has been used to analyze hundreds of proteins from small tissue samples, such as minimally invasive fine-needle aspirates from cancer tissue.[1] Although promising, the technique has not yet been extensively proven or developed.[2]

The method involves optical mapping of DNA sequences that are added as "barcodes" to antibodies attached to the cells in the sample. The method showed high reproducibility and achieved single-cell sensitivity.[1] In addition to profiling cancer cells, the method shows promise as a clinical tool to identify pathway responses to molecularly targeted drugs and to predict drug response in patient samples.[1]

References
  1. Ullal AV, Peterson V, Agasti SS, et al. (Jan 2014). "Cancer cell profiling by barcoding allows multiplexed protein analysis in fine-needle aspirates". Sci. Transl. Med. 6 (219): 219. doi:10.1126/scitranslmed.3007361. PMC 4063286. PMID 24431113.
  2. Abby Olena (2014), "Next Generation: Cancer Cell Protein Profiling: Antibody barcoding allows scientists and clinicians to analyze protein expression in small amounts of cancer tissue", The Scientist


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