Aquifex pyrophilus

Aquifex pyrophilus is a gram-negative, non-spore forming, rod-shaped bacteria. It is one of a handful of species in the Aquificota phylum, which are a group of thermophilic bacteria that are found near underwater volcanoes or hot springs.[1][2]

Aquifex pyrophilus
Scientific classification Edit this classification
Domain: Bacteria
Phylum: Aquificota
Order: Aquificales
Family: Aquificaceae
Genus: Aquifex
Species:
A. pyrophilus
Binomial name
Aquifex pyrophilus
Huber and Stetter, 1992

Name meaning

Aquifex pyrophilus has a name that references its respiration and its habitat. The name "Aquifex" means "water-maker" in Latin. The name was assigned to A. pyrophilus because it typically uses oxygen as its respiration and water as a byproduct.[3] The name "Pyrophilus" can be broken down into "Pyro" and "Philus". "Pyro" is Latin for fire, heat, or high temperature and "Philus" is a Latin word for "loving". Therefore the term "pyrophilus" can be interpreted as "heat loving" which refers to the habitat A. pyrophilus is typically found in.

Habitat
Kolbeinsey Ridge lies between Greenland, Iceland, and the Jan Mayen Microcontinent. This is the location where Aquifex pyrophilus was discovered. It is a submarine ridge that has high volcanic activity.

The genus Aquifex is a contains some of the most thermophilic bacterium of all known bacteria. A. pyrophilus is an aquatic microbe that is typically found near underwater volcanoes, marine hydrothermal vents, and/or hot springs where temperatures are extremely high and pressure can be immense.[4] A. pyrophilus has shown resistance to ultraviolet light and ionizing radiation.[4] The Aquifex genus is rather intolerable to oxygen therefore it is only found in low-oxygenated environments. Despite this, Aquifex remains one of only aerobic bacterial hyperthermophiles known.[5] Oxygen is limited near these hydrothermal vents and underwater volcanos because of the extremely high temperature and reducing power of volcanic gases like H2S. A. pyrophilus thrives where the oxic and anoxic zones meet in these environments because of the high availability of hydrogen and thiosulfate.[6]

Aquifex pyrophilus was discovered at Kolbeinsey Ridge, North of Iceland by Robert Huber and Karl Stetter in 1992.[6] In Huber's and Stetter's first analysis of the bacteria, they found that A. pyrophilus had an optimum pH of 6.8 but ranged anywhere between 5.4 and 7.5, grew at temperatures ranging from 67°C to 95°C with an optimum at 85°C, and had optimal growth with a NaCl concentration of 3% (range of 1-5%).[6]

A. pyrophilus is 2 to 6 micrometers long and has a diameter of around half a micrometer. It has eight polytrichously inserted flagella making it motile.[6] A. pyrophilus can grow singly, in pairs, and/or form large cell conglomerations, comprising up to 100 individual cells.[6][7]

Metabolism
The Aquifex electron transport chain when growing in an aerobic environment.

A. pyrophilus is strictly chemolithoautotrophic.[6] It is capable of using oxygen in its respiration, but can also grow anaerobically by nitrate reduction.[6] In aerobic conditions, A. pyrophilus oxidizes molecular hydrogen and yields H2O and ATP. Other electron donors that A. pyrophilus uses in aerobic conditions are S° and S2O32-.[6] Byproducts include sulfuric acid from S° and S2O32- and hydrogen sulfide from S° and H2.[6]

Lineage and phylogeny

A. pyrophilus has a relatively small genome. Out of the known thermophile genomes, A. pyrophilus is the smallest.[8] Due to its small genome, its ability to survive in extreme heat, its ability to be resistant toward ultraviolet light and ionizing radiation, and because of phylogenetic analyses of the small-subunit 16S rRNA gene, A. pyrophilus is thought to be one of the oldest species in the bacteria domain.[9][10]

Investigations of the phylogenetic position of Aquificales have utilized the concatenated proteins shared within the Aquificales order and A. pyrophilus.[11] The results show that the Aquificales are most closely related to Thermotogales when observing whole-genome information.[11] Additionally, Aquificales were found to be somewhat closely related to ε-proteobacteria in phylogenetic trees.[11] This slight ambiguity in the placement of Aquificales on the phylogenetic tree leaves room for some more research in this field. Despite this, there is overwhelming evidence that Aquificales, including A. pyrophilus, are some of the earliest bacteria to branch from archaea.[10]This has led to hypothesis that these bacteria evolved when the earth was still hot and had a thin atmosphere.[4]

References
  1. "aquifex". 2005-03-12. Archived from the original on 2005-03-12. Retrieved 2022-09-13.
  2. Beblo, Kristina; Douki, Thierry; Schmalz, Gottfried; Rachel, Reinhard; Wirth, Reinhard; Huber, Harald; Reitz, Günther; Rettberg, Petra (2011-11-01). "Survival of thermophilic and hyperthermophilic microorganisms after exposure to UV-C, ionizing radiation and desiccation". Archives of Microbiology. 193 (11): 797–809. doi:10.1007/s00203-011-0718-5. ISSN 1432-072X. PMID 21638055. S2CID 2281391.
  3. "Aquifex pyrophilus - microbewiki". microbewiki.kenyon.edu. Retrieved 2022-09-12.
  4. "4.5: Deeply Branching Bacteria". Biology LibreTexts. 2016-07-10. Retrieved 2022-10-11.
  5. PRATT, C (1997). "£24.95Michael T. Madigan, John M. Martinko and Jack Parker, Getting the bug for microorganisms (8th edn), , Prentice Hall (1997) ISBN 0 13 571 2254, p. 986". Trends in Cell Biology. 7 (9): 375–376. doi:10.1016/s0962-8924(97)83479-4. ISSN 0962-8924.
  6. Huber, Robert; Wilharm, Thomas; Huber, Dagmar; Trincone, Antonio; Burggraf, Siegfried; König, Helmut; Reinhard, Rachel; Rockinger, Ingrid; Fricke, Hans; Stetter, Karl O. (1992). "Aquifex pyrophilus gen. nov. sp. nov., Represents a Novel Group of Marine Hyperthermophilic Hydrogen-Oxidizing Bacteria". Systematic and Applied Microbiology. 15 (3): 340–351. doi:10.1016/S0723-2020(11)80206-7.
  7. Dworkin, Martin (1999). The Prokaryotes : an evolving electronic resource for the microbiological community. Springer-Verlag. ISBN 0-387-14254-1. OCLC 421690865.
  8. Shao, Z; Mages, W; Schmitt, R (1994). "A physical map of the hyperthermophilic bacterium Aquifex pyrophilus chromosome". Journal of Bacteriology. 176 (21): 6776–6780. doi:10.1128/jb.176.21.6776-6780.1994. ISSN 0021-9193. PMC 197038. PMID 7961434.
  9. Deckert, Gerard; Warren, Patrick V.; Gaasterland, Terry; Young, William G.; Lenox, Anna L.; Graham, David E.; Overbeek, Ross; Snead, Marjory A.; Keller, Martin; Aujay, Monette; Huber, Robert; Feldman, Robert A.; Short, Jay M.; Olsen, Gary J.; Swanson, Ronald V. (1998). "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus". Nature. 392 (6674): 353–358. doi:10.1038/32831. ISSN 1476-4687. PMID 9537320. S2CID 4413967.
  10. Burggraf, S.; Olsen, G. J.; Stetter, K. O.; Woese, C. R. (1992-08-01). "A Phylogenetic Analysis of Aquifex pyrophilus". Systematic and Applied Microbiology. 15 (3): 352–356. doi:10.1016/S0723-2020(11)80207-9. ISSN 0723-2020.
  11. Oshima, Kenro; Chiba, Yoko; Igarashi, Yasuo; Arai, Hiroyuki; Ishii, Masaharu (2012-07-12). "Phylogenetic Position of Aquificales Based on the Whole Genome Sequences of Six Aquificales Species". International Journal of Evolutionary Biology. 2012: 859264. doi:10.1155/2012/859264. ISSN 2090-8032. PMC 3403428. PMID 22844640.
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