Brassinolide

Brassinosteroids, particularly the potent brassinolide, play a crucial role in controlling various plant processes such as germination, aging, and the ability to withstand environmental and biological stresses.[6] Because of this, researchers from around the world have extensively studied model organisms like Catharanthus roseus and Arabidopsis since they were first isolated in 1979.[7] These organisms have been thoroughly examined, from how they receive brassinolide signals to how these signals affect gene expression.

In Arabidopsis, the process begins with the BRI1 receptor (Brassinosteroid Insensitive 1 receptor). This receptor is a type of protein called a leucine-rich receptor kinase and allows brassinolide to attach to it from outside the cell. This binding causes a change in the receptor's shape, and it then interacts with another protein called BRI1 associated receptor kinase 1 (BAK1). This interaction results in both proteins being chemically modified by the addition of phosphate groups in a process called phosphorylation. This, in turn, sets off a chain reaction within the cell, activating some proteins and inhibiting others, including various kinases, phosphatases, and transcription factors. Among the activated proteins are the BR signaling kinases (BSK1, BSK2, and BSK3). Their activation, in turn, activates the phosphatase BRI1 suppressor1 (BSU1), which removes a phosphate group from another protein called brassinosteroid insensitive 2 (BIN2). Removing this phosphate group inactivates BIN2, an important enzyme. As a result, protein phosphotase 2A (PP2A) can remove phosphate groups from two transcription factors, brassinazole-resistant-1 (BZR1) and BRI1-EMS-suppressor-1 (BES1), allowing them to accumulate within the cell's nucleus. There, they control the expression of specific target genes, which are involved in various cellular processes. However, when there's no brassinolide around, a regulator called BRI1 kinase inhibitor (BKI1) prevents the BRI1 receptor from interacting with the BAK1 co-receptor. This prevents the activation of BIN2, causing BZR1 and BES1 to be chemically modified by adding phosphate groups. These modified transcription factors then interact with a protein called 14-3-3 and accumulate in the cell's cytoplasm. Eventually, they are broken down and degraded by a 26S proteasome. In this way, BIN2 kinase serves as an essential negative regulator, dampening the activity of the central transcription factors BES1 and BZR1.[6]

The two transcription factors BES1 and BZR1 regulate a large number of genes involved in the synthesis of hormones, growth processes and stress response. In addition, there is inhibition of BR biosynthesis, which is evident as early as 15 minutes after brassinolide treatment.[8] Thus, the expression of several biosynthetic genes such as the CPD, DWF4 and CYP85A2 gene is inhibited.[6] These encode for brassinolide biosynthetic enzymes, thus the CPD gene encodes for the cytochrome P450 monooxygenase, which in the late C6 oxidation pathway oxidizes 6-deoxo-cathasterone to 6-deoxo teasterone and in the early C6 oxidation step catalyzes the reaction of cathasterone to teasterone. The enzyme CYP85A2 catalyzes the final biosynthesis step namely the Baeyer-Villiger lactonization of castasterone to brassinolide. This negative feedback loop ensures homeostasis of the hormone brassinolide.

Situation without bonded brassinolide

Brassinolide binds at BRI1

Brassinolide induces genes that promote growth such as TCH4 and SAUR-Ac. The gene SAUR-Ac stands for small auxin upregulated RNAs, these belong to the auxin hormone induced genes and provide cell expansion.[9] Thus, SAURs inhibit the phosphatase PP2C-D so that the plasma membrane H+-ATPase is not dephosphorylated. The active phosphorylated proton pump can thus establish an electrochemical gradient in the cell wall. The acidity increases from 6 to 4.5-6 and according to the acid growth hypothesis, it ensures the activation of expansins that cleave the bond of cellulose and hemicellulose.[10] TCH4 was identified as xyloglucan endotransglycosylase (XETs) by sequence analysis and enzyme activity. Its main function is the modification of cell walls.[11] Thus, hemicellulose is composed of xyloglucans, which is built from 1,4-ß-linked glucose polymers with lateral 1,6-ß-linked xylene residues.[12] The xylogucans can form hydrogen bonds with the cellulose microfibrils and thus structurally stabilize the cell wall. This means XET can modify the cell wall structure. It cleaves xyloglucan molecules, stores some of the energy, and then consumes it again after expansion for linking. Thus, during cell migration expansion, XET can further loosen the cell wall, which provides for the absorption of water. The resulting internal pressure (turgor) is compensated for by the cell wall expansion, so that after re-linking the result is an expanded cell.[11]

Brassinolide and gibberellin are both interdependent. BZR1 in Arabidsopsis inhibits DELLAs a negative regulator of gibberellin transduction. Thus, the activity at the promoter for binding is reduced, so gibberellin promotes cell elongation. And DELLAs interaction provides a decrease in BZR1 binding affinity to DNA.

Brassinolide inhibits jasmonic acid (JA) induced pathogen defensive for an ideal trade-off between growth and defense. Here, the transcription factor BES1 inhibits gene expression of PDF1.2a and PDF1.2b to reduce that of antimicrobial protein defensins. Furthermore, BES1 interacts with the transcription factors MYBs and reduces their activity to reduce glucosinolate (GS) biosynthesis, which is an important precursor for defense substances against predators.[13]