C16orf58

Chromosome 16 open reading frame 58, or C16orf58, also known as FLJ13638 is a protein which in humans is encoded by the C16orf58 gene.[5] The gene itself is 18892 bp long, with mRNA of 2760 bp, and a protein sequence of 468 amino acids. There is a conserved domain of unknown, DUF647. No function has been determined for this gene yet, but it is predicted that it resides in the endoplasmic reticulum in the cytoplasm.[6]

RUSF1
Identifiers
AliasesRUSF1, RUS, chromosome 16 open reading frame 58, C16orf58, RUS family member 1
External IDsMGI: 2384572 HomoloGene: 11232 GeneCards: RUSF1
Orthologs
SpeciesHumanMouse
Entrez

64755

233913

Ensembl

ENSG00000140688

ENSMUSG00000030780

UniProt

Q96GQ5

Q91W34

RefSeq (mRNA)

NM_022744

NM_145590
NM_001360882

RefSeq (protein)

NP_073581

NP_663565
NP_001347811

Location (UCSC)Chr 16: 31.49 – 31.51 MbChr 7: 127.87 – 127.9 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Species distribution

C16orf58 has very interesting conservation in that it has orthologs back through plants and fungi. However, it has not been found in reptiles, birds, or amphibians. The below table shows some, but not all, orthologs which were found using BLAST.[7]

Species Organism Common Name NCBI Accession Sequence Identity E-value Length (AAs) Gene Common Name
Homo sapiensHumanNP_073581100%0.0468C16orf58
Equus CaballusHorseXP_00149551085%0.0468PREDICTED: similar to UPF0420 protein C16orf58
Canis familiarisDogXP_54705485%0.0485similar to CG10338-PA
Mus musculusMouseQ91W3481%0.0466cDNA sequence BC017158
Monodelphis domesticaOpossumXP_00137039465%3e−160466PREDICTED: hypothetical protein
Danio rerioZebrafishNP_00110392353%4e−112432hypothetical protein LOC555936
Drosophila melanogasterFlyNP_60989740%3e−69395CG10338
Arabidopsis thalianaThale CressAAF8128437%2e−68403Contains similarity to CG10338 gene product from Drosophila melanogaster
Gallus gallusChickenNP_98982325%0.361434protein tyrosine phosphatase, receptor type, U
Xenopus tropicalisFrogAAI2205831%3.4268Stk19 protein
Saccharomyces cerevisiaeYeastEDZ7337925%0.211578YDL140Cp-like protein
Caenorhabditis elegansNematodeNP_50230019%3.0414hypothetical protein M18.6

Protein Interactions

Though the function is still unknown, C16orf58 has been shown to interact with three different proteins:

Structure

Although there are several sites that will give predictions on protein structure, C16orf58 does not have a known structure yet. That being said there is at least one transmembrane domain, if not more. Within the protein structure there are several extended areas with uncharged amino acids, these could be possible transmembrane domains, or hydrophobic cores.[6] The below shows the charge of each of the amino acids in the protein sequence, + for positive, - for negative and 0 for uncharged. Note the large segments of uncharged amino acids appear bolded. These stretches of uncharged amino acids are conserved back through distant orthologs.

      1  00—000-00 000-00000- 0+00+000-0 0000-0000+ 00000+0000 +0-0+-00-0 
     61  0000000000 0000000000 000-0000-0 000000-000 0000000000 0000000000 
    121  0000+00000 0000000+-0 00000+0000 00+00+0-00 0+00+000-0 00-00000-0 
    181  0000000000 000000000+ 0000000000 +00000000+ +0000-000+ -000-00000 
    241  0000000000 0000000000 0000000000 000000+00+ 0000-000-0 +0+000+000 
    301  0+0-00-000 00+0-00000 0000000000 0000+00000 0-00000-00 0-000000-0 
    361  0000000000 0+000+000+ 0000000000 000-00000- 0—0+0+0+0 00++-00000 
    421  +-00-00-00 00+00+000- 000+0-+000 -00-0+0000 000-++00

References
  1. GRCh38: Ensembl release 89: ENSG00000140688 - Ensembl, May 2017
  2. GRCm38: Ensembl release 89: ENSMUSG00000030780 - Ensembl, May 2017
  3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. "Entrez Gene: C16orf58". Retrieved 6 May 2009.
  6. "SDSC Biology Workbench". San Diego Supercomputer Center. Retrieved 2009-05-07.
  7. "BLAST: Basic Local Alignment Search Tool". National Center for Biotechnology Information, United States National Institutes of Health. Retrieved 2009-05-07.
  8. "STRING: functional protein association networks". EMBL.de. Retrieved 2009-05-07.
  9. "Entrez Gene: MVD mevalonate (diphospho) decarboxylase". Retrieved 6 May 2009.
  10. "mint database". Archived from the original on 2006-05-06. Retrieved 2009-05-07.
  11. "Entrez Gene: BSCL2 Bernardinelli-Seip congenital lipodystrophy 2 (seipin)". Retrieved 6 May 2009.
  12. "Entrez Gene: TSC22D4 TSC22 domain family, member 4". Retrieved 6 May 2009.
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