Carnosine N-methyltransferase
In enzymology, a carnosine N-methyltransferase (EC 2.1.1.22) is an enzyme that catalyzes the chemical reaction
- S-adenosyl-L-methionine + carnosine S-adenosyl-L-homocysteine + anserine
| carnosine N-methyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.1.1.22 | ||||||||
| CAS no. | 37256-93-2 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
Thus, the two substrates of this enzyme are S-adenosyl methionine and carnosine, whereas its two products are S-adenosylhomocysteine and anserine.
This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:carnosine N-methyltransferase. This enzyme participates in histidine metabolism.
Gene
The genes encoding carnosine N-methyltransferase activity have been identified by Jakub Drozak and coworkers in 2013[1] and 2015.[2] In birds and reptiles, the enzyme is encoded by histamine N-methyltransferase-like gene (HNMT-like).[1] Importantly, the HNMT-like gene is absent from available mammalian genomes and in mammalian species, the formation of anserine is catalyzed by methyltransferase that is unrelated to the reptilian and avian enzyme and encoded by C9orf41/UPF0586 gene.[2]
Protein Nomenclature
Currently, the avian-reptilian enzyme encoded by HNMT-like gene is labeled as carnosine N-methyltransferase 2 in public databases, while the mammalian methyltransferase is named carnosine N-methyltransferase 1 (CARNMT1).
References
- Drozak J, Chrobok L, Poleszak O, Jagielski AK, Derlacz R (2013-05-21). "Molecular identification of carnosine N-methyltransferase as chicken histamine N-methyltransferase-like protein (hnmt-like)". PLOS ONE. 8 (5): e64805. Bibcode:2013PLoSO...864805D. doi:10.1371/journal.pone.0064805. PMC 3660329. PMID 23705015.
- Drozak J, Piecuch M, Poleszak O, Kozlowski P, Chrobok L, Baelde HJ, de Heer E (July 2015). "UPF0586 Protein C9orf41 Homolog Is Anserine-producing Methyltransferase". The Journal of Biological Chemistry. 290 (28): 17190–205. doi:10.1074/jbc.M115.640037. PMC 4498059. PMID 26001783.
Further reading
- McManus IR (April 1962). "Enzymatic synthesis of anserine in skeletal muscle by N-methylation of carnosine" (PDF). Journal of Biological Chemistry. 237 (4): 1207–11. doi:10.1016/S0021-9258(18)60310-2.