DEPDC7

DEP Domain Containing Protein 7 (DEPDC7) is a protein of unknown function located on chromosome 11 bases 33,015,863-33,033,581.[1] The cytogenetic location is 11p13.[1] DEPDC7 has several aliases: DEP Domain Containing 7, DJ85M6.4, Novel 58.3 KDA Protein, Protein TR2/D15, and TR2.[2] The DEPDC7 gene is 17,718 base pairs long and has 8 exons and 9 introns.[3] The gene is capable of making 6 different mRNA transcripts.[3]  Two functional domains can be found within this gene's sequence: DEP (Dishevelled, Egl-10, and Pleckstrin) domain and RhoGAP domain.[4]

DEPDC7 gene location highlighted in blue.

mRNA

Transcription produces 4 alternatively spliced variants and 2 unspliced forms.[3] Isoform 1 is the longest of all the variants containing all 8 exons.[4] It codes for 511 amino acids.[4] Northern blot analysis detected that the DEPDC7 transcript in humans was expressed highly in the liver and moderately expressed in the kidney.[1] Hypomethylation of one of the DEPDC7 introns was related to the appearance of depression symptoms.[5]

Protein
Protein structure

The protein is 511 amino acids long and has a molecular weight of 58310.[6] DEPDC7 could have a role in tumor suppression. Overexpression inhibits the proliferation of hepatoma cells blocks the cell cycle transition from the G0/G1 to S phase, and inhibits the migration and invasion of hematoma cells.[7] DEPDC7 could potentially regulate neural development and physiology  and could participate in epigenetic processes of striatal neurons.[8] Genomic rearrangement within 11p12 and 11p14 were associated with WAGR syndrome.[9]

DEP Domain

DEP domain is named after the 3 proteins it was initially found in: Dishevelled, Egl-10, and Pleckstrin.[3] Dishevelled plays a key role in the transduction of the Wg/Wnt signal.[3] This signal goes from the cell surface to the nucleus.[3] Dishevelled is a segment polarity protein needed to make coherent arrays of polarized cells and segments in embryos.[3] It also plays a role in wingless signaling. Egl-10 regulates G-protein signaling in the central nervous system.[3] Pleckstrin has 2 PH domains flanking the DEP domain and is the major substrate of protein kinase C in platelets.[3] The DEP domain within DEPDC7 was found to bind with CARMA2 and CARMA3 proteins.[10]

Paralog

DEPDC7 has a paralog: DEPDC1B, a protein coding gene.

References
  1. "OMIM Entry - * 612294 - DEP DOMAIN-CONTAINING PROTEIN 7; DEPDC7". www.omim.org. Retrieved 2019-02-11.
  2. www.genecards.org https://www.genecards.org/cgi-bin/carddisp.pl. Retrieved 2019-02-11. {{cite web}}: Missing or empty |title= (help)
  3. "AceView: gene:DEPDC7, a comprehensive annotation of human, mouse and worm genes with mRNAs or ESTsAceView". www.ncbi.nlm.nih.gov. Retrieved 2019-02-11.
  4. "Homo sapiens DEP domain containing 7 (DEPDC7), transcript variant 1, mRNA". 2018-11-22. {{cite journal}}: Cite journal requires |journal= (help)
  5. Córdova-Palomera, A.; Fatjó-Vilas, M.; Palma-Gudiel, H.; Blasco-Fontecilla, H.; Kebir, O.; Fañanás, L. (September 2015). "Further evidence of DEPDC7 DNA hypomethylation in depression: A study in adult twins". European Psychiatry. 30 (6): 715–718. doi:10.1016/j.eurpsy.2015.04.001. ISSN 0924-9338. PMID 25952135. S2CID 33225192.
  6. "The European Bioinformatics Institute < EMBL-EBI". www.ebi.ac.uk. Retrieved 2019-02-11.
  7. Liao, Zhijun; Wang, Xinrui; Wang, Xiaojiang; Li, Lisheng; Lin, Dexin (2017-10-03). "DEPDC7 inhibits cell proliferation, migration and invasion in hepatoma cells". Oncology Letters. 14 (6): 7332–7338. doi:10.3892/ol.2017.7128. ISSN 1792-1074. PMC 5755016. PMID 29344171.
  8. Liao, Zhijun; Wang, Xinrui; Lin, Dexin; Zou, Quan (2016-03-25). "Construction and Identification of the RNAi Recombinant Lentiviral Vector Targeting Human DEPDC7 Gene". Interdisciplinary Sciences: Computational Life Sciences. 9 (3): 350–356. doi:10.1007/s12539-016-0162-y. ISSN 1913-2751. PMID 27016254. S2CID 7150784.
  9. Seabra, Catarina M; Quental, Sofia; Neto, Ana Paula; Carvalho, Filipa; Gonçalves, João; Oliveira, João Paulo; Fernandes, Susana; Sousa, Mário; Barros, Alberto (September 2014). "A novel Alu-mediated microdeletion at 11p13 removes WT1 in a patient with cryptorchidism and azoospermia". Reproductive BioMedicine Online. 29 (3): 388–391. doi:10.1016/j.rbmo.2014.04.017. ISSN 1472-6483. PMID 24912414.
  10. D′ Andrea, Egildo Luca; Ferravante, Angela; Scudiero, Ivan; Zotti, Tiziana; Reale, Carla; Pizzulo, Maddalena; De La Motte, Luigi Regenburgh; De Maio, Chiara; Mazzone, Pellegrino (2014-12-26). "The Dishevelled, EGL-10 and Pleckstrin (DEP) Domain-Containing Protein DEPDC7 Binds to CARMA2 and CARMA3 Proteins, and Regulates NF-κB Activation". PLOS ONE. 9 (12): e116062. Bibcode:2014PLoSO...9k6062D. doi:10.1371/journal.pone.0116062. ISSN 1932-6203. PMC 4277425. PMID 25541973.
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