Deproteination

Deproteination is the process of eliminating protein from some live materials. This procedure is particularly common in studies on the inorganic part of bone, teeth and shells.

Scanning Electronic micrography 10000 magnification of Bone mineral

Several different methods can be used for deproteination, such as the method that uses hydrazine,[1][2] the method that uses NaOH and the method that uses enzymes.

References
  1. Termine, J. D., Eanes, E. D., Greenfie.Dj, Nylen, M. U. & Harper, R. A. (1973) Hydrazine-deproteinated bone mineral - physical and chemical properties. Calcified Tissue Research 12: 73-90.
  2. Bertazzo, S. & Bertran, C. A. (2008) Effect of hydrazine deproteination on bone mineral phase: A critical view. Journal of Inorganic Biochemistry 102: 137-145.
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