Diafiltration

Diafiltration is a dilution process that involves removal or separation of components (permeable molecules like salts, small proteins, solvents etc.,) of a solution based on their molecular size by using micro-molecule permeable filters in order to obtain pure solution.[1][2][3][4][5][6][7]

Diagram of a diafiltration device

References
  1. Shao, Jiahui; Zydney, Andrew L. (2004). "Optimization of Ultrafiltration/Diafiltration Processes for Partially Bound Impurities". Biotechnology and Bioengineering. 87 (3): 286–292. doi:10.1002/bit.20113. PMID 15281103.
  2. Blatt, W.F.; Robinson, S.M.; Bixler, Harris J. (Oct 1968). "Membrane ultrafiltration: The diafiltration technique and its application to microsolute exchange and binding phenomena". Analytical Biochemistry. 26 (1): 151–173. doi:10.1016/0003-2697(68)90039-0. PMID 5761168.
  3. Schwartz, Larry. "Diafiltration: A Fast, Efficient Method for Desalting, or Buffer Exchange of Biological Samples". Literature Library.
  4. "Protein Concentration and Diafiltration by Tangential Flow Filtration" (PDF). Millipore.
  5. Sweeney, Scott F.; Woehrle, Gerd H.; Hutchison, James E. (2006). "Rapid Purification and Size Separation of Gold Nanoparticles via Diafiltration". Journal of the American Chemical Society. 128 (10): 3190–3197. doi:10.1021/ja0558241. PMID 16522099.
  6. Schwartz, Larry. "Diafiltration for Desalting or Buffer Exchange" (PDF). BioProcess International.
  7. "Diafiltration (Buffer Exchange) Using Hollow Fiber Membranes instead of Dialysis Tubing - Automated Diafiltration" (PDF).

Further reading
  • Bowen, W. Richard; Mohammad, A. Wahab (Aug 1998). "Diafiltration by nanofiltration: Prediction and optimization". AIChE Journal. 44 (8): 1799–1812. doi:10.1002/aic.690440811.
  • Limayem, Imène; Charcosset, Catherine; Fessi, Hatem (2004). "Purification of nanoparticle suspensions by a concentration/diafiltration process". Separation and Purification Technology. 38 (1): 1–9. doi:10.1016/j.seppur.2003.10.002.
  • Sheth, Jignesh P.; et al. (2003). "Nanofiltration-based diafiltration process for solvent exchange in pharmaceutical manufacturing". Journal of Membrane Science. 211 (2): 251–261. doi:10.1016/s0376-7388(02)00423-4.
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