FAM114A1

Protein FAM114A1 also known as nervous system overexpressed protein 20 (NOXP20) is a protein that in humans is encoded by the FAM114A1 gene.[4] Orthologs of FAM114A1 can be found in organisms as taxonomically distant from Homo sapiens as Drosophila. However, as expected, human FAM114A! is more like that of primates than any other orthologs. FAM114A1 has one paralog, FAM114A2, which also encodes a protein of unknown function.

FAM114A1
Identifiers
AliasesFAM114A1, Noxp20, family with sequence similarity 114 member A1
External IDsMGI: 1915553 HomoloGene: 12259 GeneCards: FAM114A1
Orthologs
SpeciesHumanMouse
Entrez

92689

68303

Ensembl

ENSG00000197712

ENSMUSG00000029185

UniProt

Q8IWE2

Q9D281

RefSeq (mRNA)

NM_138389
NM_001330764

NM_026667

RefSeq (protein)

NP_080943

Location (UCSC)n/aChr 5: 65.13 – 65.2 Mb
PubMed search[2][3]
Wikidata
View/Edit HumanView/Edit Mouse

Gene

FAM114A1 is located on the short arm of Chromosome 4 (4.p14) in humans on the forward strand sense, it starts at base pair 38869354 and ends at 38947365.[4] Its mRNA has 4138 bp. The gene has the following neighbors on the same chromosome:

TLR1: Toll-like receptor (TLR) 1 plays a role in pathogen recognition and activation of innate immunity.
TLR6: Toll-like receptor (TLR) 6 plays a role in pathogen recognition and activation of innate immunity.
TMEM156: The gene codes for a transmembrane protein.
KLHL5: Kelch-like 5, its protein is thought to have a role in lymphocyte activation.[5]
Gene Neighbor

Homology

Genus and species Common name Accession number Seq. length Seq. identity Seq. similarity
Pan troglodytes Chimpanzee XP_517149.2 563 a 99% 99%
Macaca mulatta Rhesus macaque EHH25809.1 563 a 97% 98%
Nomascus leucogenys Northern white-cheeked gibbon XP_003258632.1 562 a 97% 98%
Macaca fascicularis Crab-eating macaque EHH53615.1 563 a 96% 98%
Pongo abelii Sumatran orangutan XP_002814719.1 563 a 98% 96%
Equus caballus Horse XP_001498667.1 562 a 88% 93%
Loxodonta africana African bush elephant XP_003411349.1 558 a 85% 91%
Heterocephalus glaber Naked mole rat EHB16215.1 561 a 86% 90%
Cavia porcellus Guinea pig XP_003471670.1 569 a 86% 90%
Bos taurus Cow XP_588946.3 563 a 84% 90%
Sus scrofa Wild boar XP_003128969.1 562 a 84% 90%
Ailuropoda melanoleuca Giant panda XP_002928170.1 570 a 83% 89%
Canis lupus familiaris Dog XP_536261.3 560 a 83% 88%
Rattus norvegicus Rat XP_573600.2 567 a 78% 83%
Mus musculus Mouse BAB30694.1 569 a 77% 83%
Monodelphis domestica Gray short-tailed opossum XP_001374188.1 562 a 72% 81%
Meleagris gallopavo Wild turkey XP_003205919.1 563 a 64% 76%
Gallus gallus Chicken XP_423859.3 561 a 64% 75%
Taeniopygia guttata Zebra finch XP_002189709.1 565 a 61% 75%
Anolis carolinensis Carolina anole XP_003226293.1 539 a 61% 75%
Danio rerio Zebra fish NP_001082947.1 546 a 57% 72%
Xenopus (silurana) tropicalis Western clawed frog XP_002938704.1 424 a 66% 79%

Expression

NOXP20 is over-expressed in the brain,[6] microarray data [7] using the Allen Brain Atlas provides evidence of that expression. Data from NCBI GEO Profile [8] shows that although FAM114A1 is expressed in the brain, its expression goes beyond the nervous tissue to include most of the tissue types in the human body. GEO Profiles also show that FAM114A1 is more expressed in mesenchyme stem cells than in undifferentiated stem cells. Further experiments [8] have shown that there are certain factors that affect the expression of FAM114A1. One example is the direct relation between the over-expression of CLDN-1 and the over-expression of FAM114A1.

Protein

NOXP20 is made up of 563 amino acids and weighs 60742 Da with an iso-electric point of 4.415999. Little is known about the details of this protein, however, there is a good deal of scientific predictions for the protein's structure and function. Like any other protein, this protein undergoes post-translational modifications. The modification that has been proven to be true is phosphorylation on two of the protein's amino acids 196 and 199.

Structure

There are several tools available to predict the secondary structure of a protein. One tool that combines the results of few of them is PELE on SDSC Biology WorkBench.[9] According to this tool, the protein's secondary structure is mostly alpha helices and coils with some beta strands around the structure.

Predicted Secondary Structure of NOXP20 (based on results from PELE)

Interactions

There is not proof of any interactions that the FAM114A1 protein has with other proteins in the human body. However, an interaction between FAM114A1 and CDGSH iron sulfur domain 2 was detected in mice.[10] There is 77% identity and 83% similarity between the amino acids of NOXP20 in the two species (Homo sapiens and Mus musculus). Due to the close relation between the two species we can assume that NOXP20 has the same interaction in humans.

Function

The exact function of NOXP20 is still not well understood. However, there has been evidence that the protein carries a caspase recruiting domain on it.[6] Knowing that caspase is involved in apoptosis, this information leads us to believe that NOXP20 could have a role in apoptosis and regulation of cell proliferation.

References

  1. GRCm38: Ensembl release 89: ENSMUSG00000029185 - Ensembl, May 2017
  2. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  3. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "FAM114A1 family with sequence similarity 114, member A1 [Homo sapiens]". NCBI. Retrieved 22 April 2012.
  5. "GeneCards". Genecards. Retrieved 28 April 2012.
  6. Boucquey M, De Plaen E, Locker M, Poliard A, Mouillet-Richard S, Boon T, Kellermann O (Oct 2006). "Noxp20 and Noxp70, two new markers of early neuronal differentiation, detected in teratocarcinoma-derived neuroectodermic precursor cells". Journal of Neurochemistry. 99 (2): 657–69. doi:10.1111/j.1471-4159.2006.04093.x. PMID 17029606.
  7. "Micro-Array Data". Allen Institute for Brain Science. Retrieved 28 April 2012.
  8. "Geo Profiles". NCBI. Retrieved 28 April 2012.
  9. "Biology Workbench". SDSC Biology WorkBench. Retrieved 28 April 2012.
  10. "String". STRING9.0. Retrieved 28 April 2012.

Further reading

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