Facultative anaerobic organism

A facultative anaerobic organism is an organism that makes ATP by aerobic respiration if oxygen is present, but is capable of switching to fermentation if oxygen is absent.[1][2]

Aerobic and anaerobic bacteria can be identified by growing them in test tubes of thioglycolate broth:
1: Obligate aerobes need oxygen because they cannot ferment or respire anaerobically. They gather at the top of the tube where the oxygen concentration is highest.
2: Obligate anaerobes are poisoned by oxygen, so they gather at the bottom of the tube where the oxygen concentration is lowest.
3: Facultative anaerobes can grow with or without oxygen because they can metabolise energy aerobically or anaerobically. They gather mostly at the top because aerobic respiration generates more ATP than fermentation.
4: Microaerophiles need oxygen because they cannot ferment or respire anaerobically. However, they are poisoned by high concentrations of oxygen. They gather in the upper part of the test tube but not the very top.
5: Aerotolerant anaerobes do not require oxygen as they use fermentation to make ATP. Unlike obligate anaerobes, they are not poisoned by oxygen. They can be found evenly spread throughout the test tube.

Some examples of facultatively anaerobic bacteria are Staphylococcus spp.,[3] Escherichia coli, Salmonella, Listeria spp.,[4] Shewanella oneidensis and Yersinia pestis. Certain eukaryotes are also facultative anaerobes, including fungi such as Saccharomyces cerevisiae[5] and many aquatic invertebrates such as nereid polychaetes.[6]

It has been observed that in mutants of Salmonella typhimurium that underwent mutations to be either obligate aerobes or anaerobes, there were varying levels of chromatin-remodeling proteins. The obligate aerobes were later found to have a defective DNA gyrase subunit A gene (gyrA), while obligate anaerobes were defective in topoisomerase I (topI). This indicates that topoisomerase I and its associated relaxation of chromosomal DNA is required for transcription of genes required for aerobic growth, while the opposite is true for DNA gyrase.[7] Additionally, in Escherichia coli K-12 it has been noted that phosphofructokinase (PFK) exists as a dimer under aerobic conditions and as a tetramer under anaerobic conditions. Given PFK’s role in glycolysis, this has implications for the effect of oxygen on the glucose metabolism of E. coli K-12 in relation to the mechanism of the Pasteur effect.[8][9]

There may exist a core network of transcription factors (TFs) that includes the major oxygen-responsive ArcA and FNR control the adaptation of Escherichia coli to changes in oxygen availability. Activities of these two regulators are indicative of spatial effects that may affect gene expression in the microaerobic range. It has also been observed that these oxygen-sensitive proteins are protected within the cytoplasm by oxygen consumers within the cell membrane, known as terminal oxidases.[10]

Functions

Facultative anaerobes are able to grow in both the presence and absence of oxygen due to the expression of both aerobic and anaerobic respiratory chains using either oxygen or an alternative electron acceptor.[11] For example, in the absence of oxygen, E. coli can use fumarate, nitrate, nitrite, dimethyl sulfoxide, or trimethylamine oxide as an electron acceptor.[11] This flexibility allows facultative anaerobes to survive in a number of environments, and in environments with frequently changing conditions.[1]

Several species of protists use a facultative anaerobic metabolism to enhance their ATP production, and some can produce dihydrogen through this process.[12]

As pathogens

Since facultative anaerobes are able to grow in both the presence and absence of oxygen, they can survive in many different environments, adapt easily to changing conditions, and thus have a selective advantage over other bacteria. As a result, most life-threatening pathogens are facultative anaerobes.[1]

The ability of facultative anaerobic pathogens to survive without oxygen is important since their infection is shown to reduce oxygen levels in their host's gut tissue.[13] Moreover, the ability of facultative anaerobes to limit oxygen levels at infection sites is beneficial to them and other bacteria, as dioxygen can form reactive oxygen species (ROS). These species are toxic to bacteria and can damage their DNA, among other constituents.[1]

a) Escherichia coli
b) Salmonella
c) Streptococcus pneumoniae
d) Haemophilus influenzae
Examples of pathogenic facultative anaerobes.

See also

References
  1. André, Antonin C.; Debande, Lorine; Marteyn, Benoit S. (August 2021). "The selective advantage of facultative anaerobes relies on their unique ability to cope with changing oxygen levels during infection". Cellular Microbiology. 23 (8): e13338. doi:10.1111/cmi.13338. ISSN 1462-5814. PMID 33813807. S2CID 233027658.
  2. Müller, Volker (2001-04-19). "Bacterial Fermentation". eLS. doi:10.1038/npg.els.0001415. ISBN 9780470016176.
  3. Ryan KJ; Ray CG, eds. (2004). Sherris Medical Microbiology (4th ed.). McGraw Hill. pp. 261–271, 273–296. ISBN 0-8385-8529-9.
  4. Singleton P (1999). Bacteria in Biology, Biotechnology and Medicine (5th ed.). Wiley. pp. 444–454. ISBN 0-471-98880-4.
  5. Carlile MJ, Watkinson SC, Gooday GW (2001). The Fungi (2nd ed.). Academic Press. pp. 85–105. ISBN 0-12-738446-4.
  6. Schöttler, U. (November 30, 1979). "On the Anaerobic Metabolism of Three Species of Nereis (Annelida)" (PDF). Marine Ecology Progress Series. 1: 249–54. Bibcode:1979MEPS....1..249S. doi:10.3354/meps001249. ISSN 1616-1599. Retrieved February 14, 2010.
  7. Yamamoto, N., & Droffner, M. L. (1985). Mechanisms determining aerobic or anaerobic growth in the facultative anaerobe Salmonella typhimurium. Proceedings of the National Academy of Sciences, 82(7), 2077-2081. https://doi.org/10.1073/pnas.82.7.2077
  8. Doelle, H. W. (1974). Dimeric and tetrameric phosphofructokinase and the Pasteur effect in Escherichia coli K-12. FEBS Lett, 49(2), 220-222. PII: 0014-5793(74)80516-8 (core.ac.uk)
  9. Pasteur L (1857). "Mémoire sur la fermentation applée lactique" [Dissertation on the fermentation called lactic]. Comptes rendus de l'Académie des Sciences (in French). 45 (913–916): 1032–1036.
  10. Rolfe, M. D., Ocone, A., Stapleton, M. R., Hall, S., Trotter, E. W., Poole, R. K., ... & Green, J. (2012). Systems analysis of transcription factor activities in environments with stable and dynamic oxygen concentrations. Open biology, 2(7), 120091. https://doi.org/10.1098/rsob.120091
  11. Unden, Gottfried; Trageser, Martin (1991). "Oxygen regulated gene expression in Escherichia coli: Control of anaerobic respiration by the FNR protein". Antonie van Leeuwenhoek. 59 (2): 65–76. doi:10.1007/BF00445650. ISSN 0003-6072. PMID 1854188. S2CID 33083164.
  12. Ginger, Michael L.; Fritz-Laylin, Lillian K.; Fulton, Chandler; Cande, W. Zacheus; Dawson, Scott C. (2010-12-01). "Intermediary Metabolism in Protists: a Sequence-based View of Facultative Anaerobic Metabolism in Evolutionarily Diverse Eukaryotes". Protist. 161 (5): 642–671. doi:10.1016/j.protis.2010.09.001. ISSN 1434-4610. PMC 3021972. PMID 21036663.
  13. Jennewein, Jonas; Matuszak, Jasmin; Walter, Steffi; Felmy, Boas; Gendera, Kathrin; Schatz, Valentin; Nowottny, Monika; Liebsch, Gregor; Hensel, Michael; Hardt, Wolf-Dietrich; Gerlach, Roman G.; Jantsch, Jonathan (December 2015). "Low-oxygen tensions found in S almonella -infected gut tissue boost S almonella replication in macrophages by impairing antimicrobial activity and augmenting S almonella virulence: Hypoxia and Salmonella replication". Cellular Microbiology. 17 (12): 1833–1847. doi:10.1111/cmi.12476. PMID 26104016.
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