ACADSB

ACADSB is a human gene that encodes short/branched chain specific acyl-CoA dehydrogenase (SBCAD), an enzyme in the acyl CoA dehydrogenase family.

ACADSB
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesACADSB, acyl-CoA dehydrogenase, short/branched chain, 2-MEBCAD, ACAD7, SBCAD, acyl-CoA dehydrogenase short/branched chain
External IDsOMIM: 600301 MGI: 1914135 HomoloGene: 1216 GeneCards: ACADSB
Orthologs
SpeciesHumanMouse
Entrez

36

66885

Ensembl

ENSG00000196177

ENSMUSG00000030861

UniProt

P45954

Q9DBL1

RefSeq (mRNA)

NM_001609
NM_001330174

NM_025826

RefSeq (protein)

NP_001317103
NP_001600

NP_080102

Location (UCSC)Chr 10: 123.01 – 123.06 MbChr 7: 131.01 – 131.05 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

It can cause short/branched-chain acyl-CoA dehydrogenase deficiency.[5]

Structure

The human ACADSB gene is located on chromosome 10; its exact localization has been identified as 10q25-q26.[6] The open reading frame (ORF) encodes a precursor protein that contains 431 amino acids; post-translational processing results in a mature protein with 399 amino acids. The cDNA is significantly similar to the cDNA of other members of the acyl-CoA dehydrogenase family; its structure is closest to that of short chain acyl-CoA dehydrogenase.[7] The structure of the catalytic pocket has also been studied; position 104 at the bottom of the substrate-binding pocket has been identified as important in determining the length of the primary carbon chain that can be accommodated. Altering residues at positions 105 and 177 have been demonstrated to affect the rate of the dehydrogenation reactions.[8]

Function

Short/branched chain acyl-CoA dehydrogenase (ACADSB) is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. Substrate specificity is the primary characteristic used to define members of this gene family. The ACADSB gene product has the greatest activity towards the short branched chain acyl-CoA derivative, (S)-2-methylbutyryl-CoA, but also reacts significantly with other 2-methyl branched chain substrates and with short straight chain acyl-CoAs.[9] The encoded protein is also involved in L-leucine catabolism.[10]

Clinical significance

Mutations in the ACADSB gene have been associated with 2-Methylbutyryl-CoA dehydrogenase deficiency (SBCADD, also known as MBD) deficiency, an autosomal recessive metabolic disorder of impaired isoleucine degradation.[11] Many mutations across the gene's 10 exons have been identified, with the mutations causing exon skipping and other transcriptional and translational errors. The disorder may be detected by MS/MS-based routine newborn screening due to the heightened presence of 2-methylbutyrylcarnitine in tissue samples.[12][13] The disorder may also be identified using urinary organic acid analysis, by detecting the presence of 2-methylbutyryl glycinuria.[10] While many individuals with a mutation in this gene may be asymptomatic, some patients have been reported to have symptoms in early infancy. Infants may experience apneic episodes, generalized muscle atrophy, hypotonia, lethargy, seizures, and delayed motor development. Patients may also experience metabolic symptoms such as hypothermia and hypoglycemia.[14] Finally, genetic polymorphisms of the ACADSB gene may also be involved in the development of hypertension in the Japanese population.[15]

References
  1. GRCh38: Ensembl release 89: ENSG00000196177 - Ensembl, May 2017
  2. GRCm38: Ensembl release 89: ENSMUSG00000030861 - Ensembl, May 2017
  3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. Andresen BS, Christensen E, Corydon TJ, Bross P, Pilgaard B, Wanders RJ, Ruiter JP, Simonsen H, Winter V, Knudsen I, Schroeder LD, Gregersen N, Skovby F (Nov 2000). "Isolated 2-methylbutyrylglycinuria caused by short/branched-chain acyl-CoA dehydrogenase deficiency: identification of a new enzyme defect, resolution of its molecular basis, and evidence for distinct acyl-CoA dehydrogenases in isoleucine and valine metabolism". American Journal of Human Genetics. 67 (5): 1095–103. doi:10.1086/303105. PMC 1288551. PMID 11013134.
  6. Arden KC, Viars CS, Fu K, Rozen R (Feb 1995). "Localization of short/branched chain acyl-CoA dehydrogenase (ACADSB) to human chromosome 10". Genomics. 25 (3): 743–5. doi:10.1016/0888-7543(95)80023-f. PMID 7759115.
  7. Rozen R, Vockley J, Zhou L, Milos R, Willard J, Fu K, Vicanek C, Low-Nang L, Torban E, Fournier B (Nov 1994). "Isolation and expression of a cDNA encoding the precursor for a novel member (ACADSB) of the acyl-CoA dehydrogenase gene family". Genomics. 24 (2): 280–7. doi:10.1006/geno.1994.1617. PMID 7698750.
  8. He M, Burghardt TP, Vockley J (Sep 2003). "A novel approach to the characterization of substrate specificity in short/branched chain Acyl-CoA dehydrogenase". The Journal of Biological Chemistry. 278 (39): 37974–86. doi:10.1074/jbc.M306882200. PMID 12855692.
  9. "Entrez Gene: acyl-CoA dehydrogenase, short/branched chain".
  10. Andresen BS, Christensen E, Corydon TJ, Bross P, Pilgaard B, Wanders RJ, Ruiter JP, Simonsen H, Winter V, Knudsen I, Schroeder LD, Gregersen N, Skovby F (Nov 2000). "Isolated 2-methylbutyrylglycinuria caused by short/branched-chain acyl-CoA dehydrogenase deficiency: identification of a new enzyme defect, resolution of its molecular basis, and evidence for distinct acyl-CoA dehydrogenases in isoleucine and valine metabolism". American Journal of Human Genetics. 67 (5): 1095–103. doi:10.1086/303105. PMC 1288551. PMID 11013134.
  11. Sass JO, Ensenauer R, Röschinger W, Reich H, Steuerwald U, Schirrmacher O, Engel K, Häberle J, Andresen BS, Mégarbané A, Lehnert W, Zschocke J (Jan 2008). "2-Methylbutyryl-coenzyme A dehydrogenase deficiency: functional and molecular studies on a defect in isoleucine catabolism". Molecular Genetics and Metabolism. 93 (1): 30–5. doi:10.1016/j.ymgme.2007.09.002. PMID 17945527.
  12. Madsen PP, Kibaek M, Roca X, Sachidanandam R, Krainer AR, Christensen E, Steiner RD, Gibson KM, Corydon TJ, Knudsen I, Wanders RJ, Ruiter JP, Gregersen N, Andresen BS (Feb 2006). "Short/branched-chain acyl-CoA dehydrogenase deficiency due to an IVS3+3A>G mutation that causes exon skipping". Human Genetics. 118 (6): 680–90. doi:10.1007/s00439-005-0070-4. PMID 16317551. S2CID 22861705.
  13. Alfardan J, Mohsen AW, Copeland S, Ellison J, Keppen-Davis L, Rohrbach M, Powell BR, Gillis J, Matern D, Kant J, Vockley J (Aug 2010). "Characterization of new ACADSB gene sequence mutations and clinical implications in patients with 2-methylbutyrylglycinuria identified by newborn screening". Molecular Genetics and Metabolism. 100 (4): 333–8. doi:10.1016/j.ymgme.2010.04.014. PMC 2906669. PMID 20547083.
  14. Gibson KM, Burlingame TG, Hogema B, Jakobs C, Schutgens RB, Millington D, Roe CR, Roe DS, Sweetman L, Steiner RD, Linck L, Pohowalla P, Sacks M, Kiss D, Rinaldo P, Vockley J (Jun 2000). "2-Methylbutyryl-coenzyme A dehydrogenase deficiency: a new inborn error of L-isoleucine metabolism". Pediatric Research. 47 (6): 830–3. doi:10.1203/00006450-200006000-00025. PMID 10832746.
  15. Kamide K, Kokubo Y, Yang J, Matayoshi T, Inamoto N, Takiuchi S, Horio T, Miwa Y, Yoshii M, Tomoike H, Tanaka C, Banno M, Okuda T, Kawano Y, Miyata T (Jan 2007). "Association of genetic polymorphisms of ACADSB and COMT with human hypertension". Journal of Hypertension. 25 (1): 103–10. doi:10.1097/HJH.0b013e3280103a40. PMID 17143180. S2CID 40885244.

This article incorporates text from the United States National Library of Medicine, which is in the public domain.


This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.