Hexadimethrine bromide

Hexadimethrine bromide (commercial brand name Polybrene) is a cationic polymer with several uses. Currently, it is primarily used to increase the efficiency of transduction of certain cells with retrovirus in cell culture.[1] Hexadimethrine bromide acts by neutralizing the charge repulsion between virions and sialic acid on the cell surface.[2] Use of Polybrene can improve transduction efficiency 100-1000 fold[3] although it can be toxic to some cell types. Polybrene in combination with DMSO shock is used to transfect some cell types such as NIH-3T3 and CHO.[3] It has other uses, including a role in protein sequencing.[4]

Hexadimethrine bromide
Names
IUPAC name
1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide
Other names
Polybrene
Identifiers
ChemSpider
  • none
ECHA InfoCard 100.209.698
EC Number
  • 684-236-5
UNII
Properties
(C13H30Br2N2)n, linear form
Molar mass variable
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Infobox references

Hexadimethrine bromide also reverses heparin anticoagulation during open-heart surgery, and it was the original reversal agents used in the 1950s and 1960s.[5] It was replaced by protamine sulfate in 1969, after it was shown that hexadimethrine bromide could potentially cause kidney failure in dogs when used in doses in excess of its therapeutic range.[6] It is still used as an alternative to protamine sulfate for patients who are sensitive to protamine, and at least one surgical center has gone back to using it as their standard reversal agent, since protamine sulfate causes at least a mild hypotensive reaction in most or all patients [5]

Hexadimethrine bromide is also used in enzyme kinetic assays in order to reduce spontaneous activation of zymogens that are prone to auto activation.

References
  1. Howard E. Davis; Jeffery R. Morgan; Martin L. Yarmush (2002). "Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent virus adsorption on the target cell membranes". Biophysical Chemistry. 97 (2): 159–172. doi:10.1016/S0301-4622(02)00057-1. PMID 12050007.
  2. Howard E. Davis; Matthew Rosinski; Jeffrey R. Morgan; Martin L. Yarmush; et al. (2004). "Charged Polymers Modulate Retrovirus Transduction via Membrane Charge Neutralization and Virus Aggregation". Biophysical Journal. 86 (2): 1234–42. Bibcode:2004BpJ....86.1234D. doi:10.1016/S0006-3495(04)74197-1. PMC 1303915. PMID 14747357.
  3. "Polybrene Infection / Transfection Reagent | TR-1003-G". www.emdmillipore.com. Retrieved 2016-12-02.
  4. Hunkapiller, M. W.; Hood, L. E. (1978-05-30). "Direct microsequence analysis of polypeptides using an improved sequenator, a nonprotein carrier (polybrene), and high pressure liquid chromatography". Biochemistry. 17 (11): 2124–2133. doi:10.1021/bi00604a016. ISSN 0006-2960. PMID 667015.
  5. Cooney, A.; Mann, T.J. (June 1999). "Recent experiences with hexadimethrine for neutralizing heparin after cardiopulmonary bypass". Anaesthesia and Intensive Care. 27 (3): 298–300. doi:10.1177/0310057X9902700314. PMID 10389567.
  6. Randsell, HT; Haller, JA; Stowens, DD; Barton, PB (1965). "Renal toxicity of polybrene (hexadimethrine bromide)". J Surg Res. 5 (5): 195–199. doi:10.1016/S0022-4804(65)80086-5. PMID 14281435.


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