Isopenicillin N N-acyltransferase

In enzymology, an isopenicillin N N-acyltransferase (EC 2.3.1.164) is an enzyme that catalyzes the chemical reaction

phenylacetyl-CoA + isopenicillin N + H2O CoA + penicillin G + L-2-aminohexanedioate
isopenicillin N N-acyltransferase
Identifiers
EC no.2.3.1.164
CAS no.54576-90-8
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
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PMCarticles
PubMedarticles
NCBIproteins

The 3 substrates of this enzyme are phenylacetyl-CoA, isopenicillin N, and H2O, whereas its 3 products are CoA, penicillin G, and L-2-aminohexanedioate.

This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. The systematic name of this enzyme class is acyl-CoA:isopenicillin N N-acyltransferase. Other names in common use include acyl-coenzyme A:isopenicillin N acyltransferase, and isopenicillin N:acyl-CoA: acyltransferase. This enzyme participates in hydrophobic penicillins biosynthesis.

References

    • Tobin MB, Fleming MD, Skatrud PL, Miller JR (1990). "Molecular characterization of the acyl-coenzyme A:isopenicillin N acyltransferase gene (penDE) from Penicillium chrysogenum and Aspergillus nidulans and activity of recombinant enzyme in Escherichia coli". J. Bacteriol. 172 (10): 5908–14. PMC 526911. PMID 2120195.
    • Aplin RT, Baldwin JE, Roach PL, Robinson CV, Schofield CJ. "Investigations into the post-translational modification and mechanism of isopenicillin N:acyl-CoA acyltransferase using electrospray mass spectrometry". Biochem. J. 294: 357–63. PMC 1134462. PMID 8396910.

    Further reading


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