Jurkat cells

Jurkat cells are an immortalized line of human T lymphocyte cells that are used to study acute T cell leukemia, T cell signaling, and the expression of various chemokine receptors susceptible to viral entry, particularly HIV.[1] Jurkat cells can produce interleukin 2, and are used in research involving the susceptibility of cancers to drugs and radiation.

History

The Jurkat cell line (originally called JM) was established in the mid-1970s from the peripheral blood of a 14-year-old boy with T cell leukemia.[2][3] Different derivatives of the Jurkat cell line that have been mutated to lack certain genes can now be obtained from cell culture banks.[4]

Examples of derivatives
  • The JCaM1.6 cell line is deficient in Lck activity due to the deletion of part of the LCK gene (exon 7) from the LCK transcript.
  • J.RT3-T3.5 cells have a mutation in the T cell receptor beta chain locus precluding expression of this chain. This affects the cells in several ways; they do not express surface CD3 or produce the T cell receptor alpha/beta heterodimer. Since they are deficient in the TCR complex, these cells are a useful tool for transfection studies using T cell receptor alpha and beta chain genes and are widely used in labs in which T cell receptor gene transfer technologies are studied.
  • The I 9.2 and I 2.1 cell lines. The I 2.1 cell line is functionally defective for FADD and the I 9.2 cell line is functionally defective for caspase-8, both defective molecules being essential to apoptosis or necroptosis of cells.
  • The D1.1 cell line does not express the CD4 molecule, an important co-receptor in the activation pathway of helper T cells.
  • The J.gamma1 subline contains no detectable phospholipase C-gamma1 (PLC-γ1) protein and therefore has profound defects in T cell receptor (TCR) calcium mobilization and activation of nuclear factor of activated T cells (NFAT, an important transcription factor in T cells).
  • J-Lat contains integrated but transcriptionally latent HIV proviruses, in which GFP replaces nef coding sequence, and a frameshift mutation in env.

Cell line contamination

Jurkat J6 cells have been found to produce a xenotropic murine leukemia virus (X-MLV) (referred to as XMRV) that could potentially affect experimental outcomes. There is no evidence that this virus can infect humans. This infection may also change the virulence and tropism of the virus by way of phenotypic mixing and/or recombination.[5]

References
  1. Abraham, Robert; Weiss, Arthur (2004). "Jurkat T cells and development of the T-cell receptor signalling paradigm". Nature. 4 (4): 301–308. doi:10.1038/nri1330. PMID 15057788. S2CID 13253575.
  2. Schwenk, Hans-Ulrich; Schneider, Ulrich (1975). "Cell cycle dependency of a T-cell marker on lymphoblasts". Blut Zeitschrift für die Gesamte Blutforschung. 31 (5): 299–306. doi:10.1007/BF01634146. ISSN 0006-5242. PMID 1103999. S2CID 19920627.
  3. Schneider U, Schwenk H, Bornkamm G (1977). "Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma". Int J Cancer. 19 (5): 621–6. doi:10.1002/ijc.2910190505. PMID 68013. S2CID 23684046.
  4. American Type Culture Collection (ATCC)
  5. Takeuchi, Y; McClure, MO; Pizzato, M (Dec 2008). "Identification of Gammaretroviruses Constitutively Released from Cell Lines Used for Human Immunodeficiency Virus Research". Journal of Virology. 82 (24): 12585–12588. doi:10.1128/JVI.01726-08. PMC 2593302. PMID 18842727.
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