KIAA0232

KIAA0232 is a nuclear phosphoserine protein which in humans is encoded by the KIAA0232 gene.

Gene

KIAA0232 is located at 4p16.1 neighboring TBC1 domain family member 14 and an uncharacterized locus. It has 10 exons which comprise its 4 known transcript variants.[1]

Chromosomal neighborhood of gene KIAA0232

Expression

KIAA0232 is expressed fairly ubiquitously, but particularly highly in the brain relative to other tissues according to GEO normal tissue expression profiling.[2] Other notable areas of high expression identified by EST profiling include nerves, umbilical cord, and parathyroid.[3]

Homology

Paralogs

There are no known paralogs of KIAA0232.

Orthologs

KIAA0232 is conserved in most animals, including mammals, reptiles, birds, amphibians, insects, and as far back as Trichnella spiralis, a species of nematode. It is not found in fungi, plants, or prokaryotes.[4]

Protein

The KIAA0232 protein is 1395 amino acids in length with a molecular weight of 154.8kDa. It has higher than average frequencies of serine and glutamic acid residues as well as several multi-serine runs that are evolutionarily conserved. It has an isoelectric point of 4.52.[5][6]

Domains

KIAA0232 is largely composed of DUF4603.[7]

Graphic alignment of KIAA0232 and DUF4603 from NCBI BLAST

Subcellular localization

KIAA0232 is predicted to have nuclear localization.[5]

Post-translational modification

KIAA0232 is predicted by the tools at ExPASy to be highly phosphorylated, with 15 tyrosine specific sites, 25 threonine specific sites, and 103 serine specific sites.[8]

Interacting proteins

KIAA0232 is experimentally known to interact with YWHAZ,[9] DYRK1A, and DYRK1B.[10] via two hybrid interaction experiments. YWHAZ is an apoptotic path regulator known to bind to phosphoserine proteins. DYRK1A/B are dual-specificity tyrosine kinases. This corroborates the post-translational modification predictions.

Clinical significance

References
  1. "KIAA0232 KIAA0232 [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2016-04-28.
  2. "NCBI GEO Expression Profiles".
  3. "EST Profile - Hs.79276". www.ncbi.nlm.nih.gov. Retrieved 2016-05-09.
  4. "BLAST: Basic Local Alignment Search Tool". blast.ncbi.nlm.nih.gov. Retrieved 2016-05-09.
  5. "SAPS, Statistical Analysis of Protein Sequence". SDSC Biology Workbench.
  6. "PI, Isoelectric point determination". SDSC Biology Workbench. Archived from the original on 2003-08-11.
  7. "RCSB Protein Data Bank". Archived from the original on 2012-12-27.
  8. "NetPhos 2.0 Server". www.cbs.dtu.dk. Retrieved 2016-05-09.
  9. Bandyopadhyay, Sourav; Chiang, Chih-yuan; Srivastava, Jyoti; Gersten, Merril; White, Suhaila; Bell, Russell; Kurschner, Cornelia; Martin, Christopher H; Smoot, Mike (2010). "A human MAP kinase interactome". Nature Methods. 7 (10): 801–805. doi:10.1038/nmeth.1506. PMC 2967489. PMID 20936779.
  10. Varjosalo, Markku; Keskitalo, Salla; Van Drogen, Audrey; Nurkkala, Helka; Vichalkovski, Anton; Aebersold, Ruedi; Gstaiger, Matthias (2013-04-25). "The Protein Interaction Landscape of the Human CMGC Kinase Group". Cell Reports. 3 (4): 1306–1320. doi:10.1016/j.celrep.2013.03.027. ISSN 2211-1247. PMID 23602568.
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