L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase
L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (EC 6.3.1.13, MshC, MshC ligase, Cys:GlcN-Ins ligase, mycothiol ligase) is an enzyme with systematic name L-cysteine:1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol ligase (AMP-forming).[1][2][3] This enzyme catalyses the following chemical reaction
- 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate
| L-cysteine:1D-myo-inositol 2-amino-2-deoxy-α-D-glucopyranoside ligase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 6.3.1.13 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
This enzyme is a key enzyme in the biosynthesis of mycothiol.
References
- Fan F, Luxenburger A, Painter GF, Blanchard JS (October 2007). "Steady-state and pre-steady-state kinetic analysis of Mycobacterium smegmatis cysteine ligase (MshC)". Biochemistry. 46 (40): 11421–9. doi:10.1021/bi7011492. PMC 2526253. PMID 17848100.
- Gutierrez-Lugo MT, Newton GL, Fahey RC, Bewley CA (November 2006). "Cloning, expression and rapid purification of active recombinant mycothiol ligase as B1 immunoglobulin binding domain of streptococcal protein G, glutathione-S-transferase and maltose binding protein fusion proteins in Mycobacterium smegmatis" (PDF). Protein Expression and Purification. 50 (1): 128–36. doi:10.1016/j.pep.2006.07.005. PMID 16908186.
- Tremblay LW, Fan F, Vetting MW, Blanchard JS (December 2008). "The 1.6 A crystal structure of Mycobacterium smegmatis MshC: the penultimate enzyme in the mycothiol biosynthetic pathway". Biochemistry. 47 (50): 13326–35. doi:10.1021/bi801708f. PMC 2628295. PMID 19053270.
External links
- L-cysteine:1D-myo-inositol+2-amino-2-deoxy-alpha-D-glucopyranoside+ligase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
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