LAPC4

LAPC4 cells are a cell line of human prostate cancer commonly used in the field of oncology. The tissue was harvested from the lymph node metastasis of a male patient with hormone refractory prostate cancer which was then xenografted into SCID mice and later harvested and plated on tissue culture dishes, where it can be propagated as an immortalized prostate cancer cell line.[1][2][3]

Representative phase-contrast image of LAPC4 cells. 32X magnification.

Characteristics

LAPC4 are a lowly adherent, epithelial cell line with high Androgen receptor and Prostate specific antigen expression.[4] Unlike the other commonly utilized, Androgen receptor positive prostate cancer cell lines LNCaP and VCaP, LAPC4 have high expression of Keratin 5, a basal marker, as well as the luminal markers Keratin 8 and Keratin 18.[4] LAPC4 also expresses mutated P53 (R175H). [4]

The cells have an approximate doubling time of 72 hours under typical culture conditions.[5]

References
  1. Klein, KA; Reiter, RE; Redula, J; Moradi, H; Zhu, XL; Brothman, AR; Lamb, DJ; Marcelli, M; Belldegrun, A; Witte, ON; Sawyers, CL (April 1997). "Progression of metastatic human prostate cancer to androgen independence in immunodeficient SCID mice". Nature Medicine. 3 (4): 402–8. doi:10.1038/nm0497-402. PMID 9095173. S2CID 21273888.
  2. Russell, Pamela J.; Kingsley, Elizabeth A. (2003). "Chapter 2: Human Prostate Cancer Cell Lines" (PDF). In Russell, Pamela J.; Jackson, Paul; Kingsley, Elizabeth A. (eds.). Prostate cancer methods and protocols. Totowa, N.J.: Humana Press. pp. 21–39. ISBN 978-1-59259-372-9.
  3. Garcia, RR; Masoodi, KZ; Pascal, LE; Nelson, JB; Wang, Z (2014). "Growth of LAPC4 prostate cancer xenograft tumor is insensitive to 5α-reductase inhibitor dutasteride". American Journal of Clinical and Experimental Urology. 2 (1): 82–91. PMC 4219295. PMID 25374909.
  4. van Bokhoven, A; Varella-Garcia, M; Korch, C; Johannes, WU; Smith, EE; Miller, HL; Nordeen, SK; Miller, GJ; Lucia, MS (1 November 2003). "Molecular characterization of human prostate carcinoma cell lines". The Prostate. 57 (3): 205–25. doi:10.1002/pros.10290. PMID 14518029. S2CID 32949167.
  5. "Cell line LAPC-4 (CVCL_4744)". Cellosaurus. Retrieved 29 January 2018.


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