Lactophenol cotton blue

Lactophenol cotton blue (LCB) is a mixture of methyl blue, a histological stain, and lactophenol (a solution of phenol, lactic acid, and glycerol in water). It is used in wet-mount preparations for visualization of fungal structures, especially in medical mycology.[1]

Fusarium oxysporum conidiophores stained by lactophenol cotton blue

Methyl blue stains fungal cell walls a bright cerulean color, while lactophenol acts as a mountant.

References
  1. J. Heritage; E. G. V. Evans; R. A. Killington (1996). Introductory Microbiology. Cambridge University Press. p. 100. ISBN 978-0-521-44977-9.
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