List of highways numbered 331

- Myrtle Street

• Manitoba Provincial Road 331
• Newfoundland and Labrador Route 331
• Nova Scotia Route 331
• Prince Edward Island Route 331

• National Route 331

• Main road 331 (Hungary)

• National Highway 331 (India)

• Japan National Route 331

• U.S. Route 331 (former)
• U.S. Route 331
• Arkansas Highway 331
• Florida:
  • Florida State Road 331
  • County Road 331A (Levy County, Florida)
• Georgia State Route 331
• Indiana State Road 331
• Kentucky Route 331
• Louisiana Highway 331
• Maryland Route 331
• M-331 (Michigan highway)
• Mississippi Highway 331 (unsigned)
• Montana Secondary Highway 331
• New York State Route 331
• Ohio State Route 331
• Oregon Route 331
• Pennsylvania Route 331
• Puerto Rico Highway 331
• Tennessee State Route 331
• Texas:
  • Texas State Highway 331 (former)
  • Texas State Highway Spur 331
  • Farm to Market Road 331
• Virginia State Route 331
• West Virginia Route 331
• Wyoming Highway 331