Marco Fraaije

Marco Wilhelmus Fraaije (born 7 December 1968) is a Dutch scientist whose research concerns enzymology of redox enzymes, enzyme discovery & engineering and biocatalysis at the Groningen Biomolecular Sciences and Biotechnology Institute (GBB) at the University of Groningen.[1][2][3][4]

Marco Fraaije (left) accepting the BIOCAT science award at BIOCAT2018

Education

Marco Fraaije graduated in 1993 with a Master of Science degree in Molecular Sciences at Wageningen University.[5] Subsequently, he became a doctoral student at Wageningen University under supervision of Willem van Berkel focusing on the mechanism and structure of flavoenzymes and was awarded his PhD in biochemistry in 1998.[6] Following his PhD, he worked as a postdoctoral researcher as EMBO fellow in the protein crystallography research group at the University of Pavia. In 1999, he was made assistant professor at GBB at the University of Groningen, and in 2007 he was appointed as associate professor. In 2012, he was made full professor in molecular enzymology.[5]

Research

Fraaije is active in the fields of enzyme engineering and biocatalysis.[1] His research mainly deals with discovery, engineering and exploration of novel oxidative enzymes, with special emphasis on flavin-containing enzymes.[7] Besides exploring the biocatalytic potential of these biocatalysts, he also aims at elucidating the molecular functioning of oxidative flavoenzymes.[2] He also has interest in evolutionary aspects of enzymology and in line with this he is board member of the geological museum Oertijdmuseum in Boxtel.[8]

Marco Fraaije has a significant number of publications and four patents.[1][9] He has coordinated EU-funded projects including OXYGREEN (2008-2013),[10] ROBOX (2015-2019),[11] and OXYTRAIN (2017-2020).[12]

Awards

In 2018, Fraaije received the BIOCAT science award from the Biocat Society at the International Congress on Biocatalysis for his scientific achievement in the field of biocatalysis.[13] Other research prizes include the Unilever research prize, 1993; EMBO long-term fellowship, 1998; and the VICI-NWO research grant, 2016.[5]

In 2005, he became a member of the Biomolecular Chemistry division of the Netherlands Organization for Scientific Research and currently chairs the Applied Biocatalysis division of the Dutch Biotechnology Society.[14]

Selected publications
  • Aalbers FS, Fraaije MW (2017) Coupled reactions by coupled enzymes: alcohol to lactone cascade with alcohol dehydrogenase-cyclohexanone monooxygenase fusions. Appl Microbiol Biotechnol. 101, 7557-7565.[15]
  • Beyer N, Kulig JK, Bartsch A, Hayes MA, Janssen DB, Fraaije MW (2017) P450BM3 fused to phosphite dehydrogenase allows phosphite-driven selective oxidations. Appl Microbiol Biotechnol. 101, 2319-2331.[16]
  • Romero E, Castellanos JR, Mattevi A, Fraaije MW (2016) Characterization and crystal structure of a robust cyclohexanone monooxygenase. Angew Chem Int Ed Engl. 55, 15852-15855. Selected as Biocatalysis Hot Paper.[17]
  • Brondani PB, Dudek HM, Martinoli C, Mattevi A, Fraaije MW (2014) Finding the switch: turning a Baeyer-Villiger monooxygenase into a NADPH oxidase. J. Am. Chem. Soc. 136, 16966-16969 [18]

References
  1. "Marco Fraaije - Google Scholar Citations". scholar.google.nl. Retrieved 2018-11-20.
  2. Romero, Elvira; Gómez Castellanos, J. Rubén; Gadda, Giovanni; Fraaije, Marco W.; Mattevi, Andrea (2018-01-11). "Same Substrate, Many Reactions: Oxygen Activation in Flavoenzymes". Chemical Reviews. 118 (4): 1742–1769. doi:10.1021/acs.chemrev.7b00650. ISSN 0009-2665. PMID 29323892.
  3. Nguyen, Quoc-Thai; Romero, Elvira; Dijkman, Willem P.; de Vasconcellos, Suzan Pantaroto; Binda, Claudia; Mattevi, Andrea; Fraaije, Marco W. (2018-01-10). "Structure-Based Engineering of Phanerochaete chrysosporium Alcohol Oxidase for Enhanced Oxidative Power toward Glycerol". Biochemistry. 57 (43): 6209–6218. doi:10.1021/acs.biochem.8b00918. ISSN 0006-2960. PMC 6210165. PMID 30272958.
  4. Mathew, Sam; Trajkovic, Milos; Kumar, Hemant; Nguyen, Quoc-Thai; Fraaije, Marco W. (2018). "Enantio- and regioselective ene-reductions using F420H2-dependent enzymes" (PDF). Chemical Communications. 54 (79): 11208–11211. doi:10.1039/C8CC04449J. ISSN 1359-7345. PMID 30230493.
  5. "Curriculum Vitae of prof. dr. ir. M.W. (Marco) Fraaije | How to find us | About us | University of Groningen". www.rug.nl. Retrieved 2018-11-20.
  6. "Thesis Marco Fraaije".
  7. Fraaije, Marco W.; Mattevi, Andrea (2000-01-03). "Flavoenzymes: diverse catalysts with recurrent features". Trends in Biochemical Sciences. 25 (3): 126–132. doi:10.1016/S0968-0004(99)01533-9. hdl:11370/861919fc-cdfe-482e-8d82-3a2ecf45029b. ISSN 0968-0004. PMID 10694883.
  8. "HOME". Dino's (in Dutch). Retrieved 2018-11-20.
  9. "Google Patents". patents.google.com. Retrieved 2018-11-20.
  10. "Effective redesign of oxidative enzymes for green chemistry (OXYGREEN) | Projects | FP7 | CORDIS | European Commission". CORDIS | European Commission. Retrieved 2018-12-19.
  11. "Expanding the industrial use of Robust Oxidative Biocatalysts for the conversion and production of alcohols (ROBOX) | Projects | H2020 | CORDIS | European Commission". CORDIS | European Commission. Retrieved 2018-11-20.
  12. "Harnessing the power of enzymatic oxygen activation | Projects | H2020 | CORDIS | European Commission". CORDIS | European Commission. Retrieved 2018-11-20.
  13. Webmaster. "Verleihung der biocat Awards 2018 auf dem 9. Internationalen Biokatalysekongress an der TUHH" (in German). Retrieved 2018-11-20.
  14. "Applied biocatalysis - NBV". nbv.kncv.nl. Retrieved 2018-11-20.
  15. Aalbers, Friso S.; Fraaije, Marco W. (2017-09-15). "Coupled reactions by coupled enzymes: alcohol to lactone cascade with alcohol dehydrogenase–cyclohexanone monooxygenase fusions". Applied Microbiology and Biotechnology. 101 (20): 7557–7565. doi:10.1007/s00253-017-8501-4. ISSN 0175-7598. PMC 5624969. PMID 28916997.
  16. Beyer, Nina; Kulig, Justyna K.; Bartsch, Anette; Hayes, Martin A.; Janssen, Dick B.; Fraaije, Marco W. (2016-11-29). "P450BM3 fused to phosphite dehydrogenase allows phosphite-driven selective oxidations". Applied Microbiology and Biotechnology. 101 (6): 2319–2331. doi:10.1007/s00253-016-7993-7. ISSN 0175-7598. PMC 5320008. PMID 27900443.
  17. Romero, Elvira; Castellanos, J. Rubén Gómez; Mattevi, Andrea; Fraaije, Marco W. (2016-11-22). "Characterization and Crystal Structure of a Robust Cyclohexanone Monooxygenase". Angewandte Chemie International Edition. 55 (51): 15852–15855. doi:10.1002/anie.201608951. ISSN 1433-7851. PMC 5213842. PMID 27873437.
  18. Brondani, Patrícia B.; Dudek, Hanna M.; Martinoli, Christian; Mattevi, Andrea; Fraaije, Marco W. (2014-01-12). "Finding the Switch: Turning a Baeyer–Villiger Monooxygenase into a NADPH Oxidase". Journal of the American Chemical Society. 136 (49): 16966–16969. doi:10.1021/ja508265b. ISSN 0002-7863. PMID 25423359.
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