Master mix (PCR)

A master mix is a mixture containing precursors and enzymes used as an ingredient in RT-PCR techniques in molecular biology. Such mixtures contain a mixture dNTPs (required as a substrate for the building of new DNA strands), MgCl2, Taq polymerase (an enzyme required to building new DNA strands), a pH buffer and come mixed in nuclease-free water.[1][2][3][4]

Master mixes for real-time PCR include a fluorescent compound (frequently SYBR green), and the choice of mix also influence test sensitivity and consistency.[5]

Differences in the choice of master mixes can sometimes explain difference in experimental results, a particular case being the measurement of telomere length.[6][7]

References
  1. "PCR Master Mix". Sigma-Aldrich. Merck.
  2. "GoTaq® G2 Master Mixes | PCR Master Mix". www.promega.com.
  3. "PCR Master Mix | Bio-Rad". www.bio-rad.com. Bio Rad.
  4. "PCR Master Mixes | Chai". www.chaibio.com. Chai.
  5. Yang, Jianxin; Kemps-Mols, Berit; Spruyt-Gerritse, Marijke; Anholts, Jacqueline; Claas, Frans; Eikmans, Michael (4 June 2016). "The source of SYBR green master mix determines outcome of nucleic acid amplification reactions". BMC Research Notes. 9 (1). doi:10.1186/s13104-016-2093-4. PMID 27259280.
  6. Jiménez, Karen M.; Forero, Diego A. (5 April 2018). "Effect of master mixes on the measurement of telomere length by qPCR". Molecular Biology Reports. 45 (4): 633–638. doi:10.1007/s11033-018-4175-y. S2CID 254833450.
  7. Lin, Jue; Smith, Dana L.; Esteves, Kyle; Drury, Stacy (January 2019). "Telomere length measurement by qPCR – Summary of critical factors and recommendations for assay design". Psychoneuroendocrinology. 99: 271–278. doi:10.1016/j.psyneuen.2018.10.005. PMC 6363640. PMID 30343983.
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