Micrograph

A micrograph or photomicrograph is a photograph or digital image taken through a microscope or similar device to show a magnified image of an object. This is opposed to a macrograph or photomacrograph, an image which is also taken on a microscope but is only slightly magnified, usually less than 10 times. Micrography is the practice or art of using microscopes to make photographs.

This article is about photography using microscopes. For images formed with tiny letters, see Micrography. For miniaturized photographs, see Microphotograph.
100x micrograph of strawberry flesh
100x light micrograph of Meissner's corpuscle at the tip of a dermal papillus.
40x micrograph of a canine rectum cross section.
A photomicrograph of a thin section of a limestone with ooids. The largest is approximately 1.2 mm in diameter. The red object in the lower left is a scale bar indicating relative size.
Approximately 10x micrograph of a doubled die on a coin, where the date was punched twice in the die used to strike the coin.

A micrograph contains extensive details of microstructure. A wealth of information can be obtained from a simple micrograph like behavior of the material under different conditions, the phases found in the system, failure analysis, grain size estimation, elemental analysis and so on. Micrographs are widely used in all fields of microscopy.

Types

Photomicrograph

A light micrograph or photomicrograph is a micrograph prepared using an optical microscope, a process referred to as photomicroscopy. At a basic level, photomicroscopy may be performed simply by connecting a camera to a microscope, thereby enabling the user to take photographs at reasonably high magnification.

Scientific use began in England in 1850 by Richard Hill Norris FRSE for his studies of blood cells.[1]

Roman Vishniac was a pioneer in the field of photomicroscopy, specializing in the photography of living creatures in full motion. He also made major developments in light-interruption photography and color photomicroscopy.

Photomicrographs may also be obtained using a USB microscope attached directly to a home computer or laptop.

Electron micrograph

An electron micrograph is a micrograph prepared using an electron microscope.

Magnification and micron bars

Micrographs usually have micron bars, or magnification ratios, or both.

Magnification is a ratio between the size of an object on a picture and its real size. Magnification can be a misleading parameter as it depends on the final size of a printed picture and therefore varies with picture size. A scale bar, or micron bar, is a line of known length displayed on a picture. The bar can be used for measurements on a picture. When the picture is resized the bar is also resized making it possible to recalculate the magnification. Ideally, all pictures destined for publication/presentation should be supplied with a scale bar; the magnification ratio is optional. All but one (limestone) of the micrographs presented on this page do not have a micron bar; supplied magnification ratios are likely incorrect, as they were not calculated for pictures at the present size.

Micrography as art

The microscope has been mainly used for scientific discovery. It has also been linked to the arts since its invention in the 17th century. Early adopters of the microscope, such as Robert Hooke and Antonie van Leeuwenhoek, were excellent illustrators. Cornelius Varley's graphic microscope made sketching from a microscope easier with a camera-lucida-like mechanism. After the invention of photography in the 1820s the microscope was later combined with the camera to take pictures instead of relying on an artistic rendering.

Since the early 1970s individuals have been using the microscope as an artistic instrument. Websites and traveling art exhibits such as the Nikon Small World and Olympus Bioscapes have featured a range of images for the sole purpose of artistic enjoyment. Some collaborative groups, such as the Paper Project have also incorporated microscopic imagery into tactile art pieces as well as 3D immersive rooms and dance performances.

In 2015, photographer and gemologist Danny J. Sanchez photographed mineral and gemstone interiors in works referred to as "otherworldly."[2][3][4]

Photomicrography in smartphones

Bellina and Missoni first published a paper in 2009 describing the method of photomicrography in a smartphone with free-hand technique.[5] An operator just need to focus her/his smartphone camera on the eyepiece of a microscope and capture the photo. However, later, various commercial and home-made adapters were introduced to ease focusing.[6] A home-made adapter was also made using scrap materials and a Coca-cola aluminum can.[7]

See also

References
  1. "Papers of Richard Hill Norris" (PDF). calmview.bham.ac.uk. Archived (PDF) from the original on 7 November 2017. Retrieved 4 November 2017.
  2. Wiley, Melissa (13 January 2015). "Surreal Photos Reveal the Otherworldly Insides of Gemstones". Smithsonian. Retrieved 1 January 2020.
  3. Bierend, Doug (13 June 2014). "Take a Trip Through the Strange Worlds Within Gemstones". Wired. Retrieved 1 January 2020.
  4. Landau, Elizabeth (26 June 2017). "Roll Your Blunts and Peer Inside These Gemstones". Vice. Retrieved 1 January 2020.
  5. Bellina, Livia; Missoni, Eduardo (19 June 2009). "Mobile cell-phones (M-phones) in telemicroscopy: increasing connectivity of isolated laboratories". Diagnostic Pathology. 4: 19. doi:10.1186/1746-1596-4-19. ISSN 1746-1596. PMC 2706795. PMID 19545373.
  6. Roy, Somak; Pantanowitz, Liron; Amin, Milon; Seethala, Raja R.; Ishtiaque, Ahmed; Yousem, Samuel A.; Parwani, Anil V.; Cucoranu, Ioan; Hartman, Douglas J. (30 July 2014). "Smartphone adapters for digital photomicrography". Journal of Pathology Informatics. 5 (1): 24. doi:10.4103/2153-3539.137728. ISSN 2229-5089. PMC 4141421. PMID 25191623.
  7. Mondal, Himel; Mondal, Shaikat; Das, Debasish (2017). "Development of a Simple Smartphone Adapter for Digital Photomicrography". Indian Dermatology Online Journal. 8 (6): 485–486. doi:10.4103/idoj.IDOJ_33_17. ISSN 2229-5178. PMC 5707845. PMID 29204396.
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