Myxomatosis

Myxomatosis is a disease caused by Myxoma virus, a poxvirus in the genus Leporipoxvirus. The natural hosts are tapeti (Sylvilagus brasiliensis) in South and Central America, and brush rabbits (Sylvilagus bachmani) in North America. The myxoma virus causes only a mild disease in these species, but causes a severe and usually fatal disease in European rabbits (Oryctolagus cuniculus).

This article is about the disease in rabbits. For the Radiohead song, see Hail to the Thief.

Myxoma virus
Myxoma virus (transmission electron microscope)
Virus classification Edit this classification
(unranked): Virus
Realm: Varidnaviria
Kingdom: Bamfordvirae
Phylum: Nucleocytoviricota
Class: Pokkesviricetes
Order: Chitovirales
Family: Poxviridae
Genus: Leporipoxvirus
Species:
Myxoma virus

Myxomatosis is an example of what occurs when a virus jumps from a species adapted to the virus to a native host, and has been extensively studied for this reason. The virus was intentionally introduced in Australia, France, and Chile in the 1950s to control wild European rabbit populations.

Cause
Rabbit suffering from Myxomatosis
Suffering from Myxomatosis

Myxoma virus is in the genus Leporipoxvirus (family Poxviridae; subfamily Chordopoxvirinae). Like other poxviruses, myxoma viruses are large DNA viruses with linear double-stranded DNA. Virus replication occurs in the cytoplasm of the cell. The natural hosts are tapeti (Sylvilagus brasiliensis) in South and Central America, and brush rabbits (Sylvilagus bachmani) in North America. The myxoma virus causes only a mild disease in these species, with signs limited to the formation of skin nodules.[1]

Myxomatosis is the name of the severe and often fatal disease in European rabbits caused by the myxoma virus. Different strains exist which vary in their virulence. The Californian strain, which is endemic to the west coast of the United States and Baja in Mexico, is the most virulent, with reported case fatality rates of 100%. The South American strain, present in South America and Central America, is slightly less virulent, with reported case fatality rates of 99.8%. Strains present in Europe and Australia have become attenuated, with reported case fatality rates of 50–95%. While wild rabbits in Europe and Australia have developed some immunity to the virus, this is not generally true of pet rabbits.[2]

Transmission

Myxomatosis is transmitted primarily by insects. Disease transmission commonly occurs via mosquito or flea bites, but can also occur via the bites of flies and lice, as well as arachnid mites. The myxoma virus does not replicate in these arthropod hosts, but is physically carried by biting arthropods from one rabbit to another. Seasonality is driven by the availability of arthropod vectors and the proximity of infected wild rabbits.[3]

The myxoma virus can also be transmitted by direct contact. Infected rabbits shed the virus in ocular and nasal secretions and from areas of eroded skin. The virus may also be present in semen and genital secretions. Poxviruses are fairly stable in the environment and can be spread by contaminated objects such as water bottles, feeders, caging, or people's hands.[3] They are resistant to drying but are sensitive to some disinfectants.[4]

Pathophysiology

A laboratory study in which European rabbits received intradermal injections of a South American strain of the myxoma virus demonstrated the following progression of disease. Initially the virus multiplied in the skin at the site of inoculation. Approximately 2 days following inoculation the virus was found in nearby lymph nodes, and at 3 days it was found in the bloodstream and abdominal organs. At approximately 4 days the virus was isolated from non-inoculated skin as well as from the testes. Slight thickening of the eyelids and the presence of virus in conjunctival fluid was detectable on day 5. Testicular engorgement was noticed on day 6.[5]

Clinical presentation in European rabbits
European rabbit with Lausanne strain of myxomatosis (West Yorkshire, UK)
European rabbit with Californian strain of myxomatosis, indicated by swollen eyelids and genitals (Santa Cruz, California)

The clinical signs of myxomatosis depend on the strain of virus, the route of inoculation, and the immune status of the host. Signs of the classic nodular form of the disease include a subcutaneous mass at the site of inoculation, swelling and edema of the eyelids and genitals, a milky or purulent ocular discharge, fever, lethargy, depression, and anorexia.

According to Meredith (2013), the typical time course of the disease is as follows:[1]

Days after infectionClinical signs
2–4Swelling at site of infection
4Fever
6Swelling of eyelids, face, base of ears, and anogenital area
6Secondary skin lesions, including red pinpoint lesions on eyelids and raised masses on body
6–8Clear ocular and nasal discharge that becomes mucopurulent and crusting
7–8Respiratory distress
8–9Hypothermia
10Complete closure of eyelids due to swelling
10–12Death

In peracute disease with a highly virulent strain, death may occur within 5 to 6 days of infection with minimal clinical signs other than the conjunctivitis. Death usually occurs between days 10 and 12. Highly virulent strains, such as those present in North and South America, have essentially 100% case fatality rates.

In rabbits infected with attenuated, less virulent strains of the virus, the lesions seen are more variable and generally milder, and the time course is delayed and prolonged. Many rabbits will survive and the cutaneous lesions gradually scab and fall off, leaving scarring. A milder form of the disease is also seen in previously vaccinated domestic rabbits that have partial immunity. Vaccinated rabbits often present with localized scabbed lesions, frequently on the bridge of the nose and around the eyes, or multiple cutaneous masses over the body. They are often still bright and alert, and survive with nursing care.[1]

Respiratory signs are a common finding in rabbits that survive the first stages of myxomatosis. Mucopurulent nasal discharge occurs, leading to gasping and stertorous respiration with extension of the head and neck. Secondary bacterial pneumonia occurs in many cases. Chronic respiratory disease, such as nasal discharge, is common in surviving rabbits. Even in apparently recovered rabbits, it is not unusual to find lung lobes filled with fluid rather than air at necropsy.[3]

Since the 1970s an "amyxomatous" form of the disease has been reported in Europe which lacks the cutaneous nodules typical of myxomatosis. This form is clinically milder and generally nonlethal. Respiratory signs, including clear or purulent nasal discharge, predominate. Perineal edema, swollen eyelids, and purulent blepharoconjunctivitis are generally still present. This form has been observed in wild rabbits, but is significant mainly in farmed rabbits.[1]

Diagnosis

Diagnosis of myxomatosis in European rabbits is often made on the basis of the characteristic clinical appearance. If a rabbit dies without exhibiting the classic signs of myxomatosis, or if further confirmation is desired, a number of laboratory tests are available. Historically these have included histopathology, electron microscopy, and virus isolation. Histopathologic examination of affected skin typically shows undifferentiated mesenchymal cells within a matrix of mucin, inflammatory cells, and edema. Intracytoplasmic inclusions may be seen in the epidermis and in conjunctival epithelium.[6]

Negative-stain electron microscopic examination can also be used for diagnosis due to the large size and distinctive structure of poxviruses. This method allows rapid visualization of poxviruses, but does not allow specific verification of virus species or variants.[7] Virus isolation remains the "gold standard" against which other methods of virus detection are compared. Theoretically at least, a single viable virus present in a specimen can be grown in cultured cells, thus expanding it to produce enough material to permit further detailed characterization.[8]

The more recent development of molecular methods such as polymerase chain reaction (PCR) and real-time polymerase chain reaction assays has created faster and more accurate methods of myxoma virus identification.[7] Real time PCR simplifies the diagnosis of myxomatosis by allowing nasal, ocular, or genital swabs to be quickly tested. It can also be used on paraffin-embedded tissue samples to confirm the presence of Myxoma virus and identify the viral strain.[9]

Treatment

At present, no specific treatment exists for myxomatosis. If the decision is made to attempt treatment, careful monitoring is necessary to avoid prolonging suffering. Previously vaccinated rabbits or those infected with an attenuated strain may recover given supportive care with fluids, food, and broad spectrum antivirals. Cessation of food and water intake, ongoing severe weight loss, or rectal temperatures below 37 C (98.6 F) are reasons to consider euthanasia.[3]

Prevention

Vaccination

Vaccines against myxomatosis are available in some countries. All are modified live vaccines based either on attenuated myxoma virus strains or on the closely related Shope fibroma virus, which provides cross-immunity. It is recommended that all rabbits in areas of the world where myxomatosis is endemic be routinely vaccinated, even if kept indoors, because of the ability of the virus to be carried inside by vectors or fomites. In group situations where rabbits are not routinely vaccinated, vaccination in the face of an outbreak is beneficial in limiting morbidity and mortality.[1] The vaccine does not provide 100% protection,[3] so it is still important to prevent contact with wild rabbits and insect vectors. Myxomatosis vaccines must be boostered regularly to remain effective, and annual vaccinations are usually recommended.[1]

In Europe and the United Kingdom a bivalent vectored vaccine called Nobivac Myxo-RHD[10] is available that protects against both myxomatosis and rabbit haemorrhagic disease. This vaccine is licensed for immunization of rabbits 5 weeks of age or older, with onset of immunity taking approximately 3 weeks. Protection against myxomatosis and rabbit hemorrhagic disease has a duration of immunity for 12 months, and annual vaccination is recommended to ensure continued protection. The vaccine has been shown to reduce mortality and clinical signs of myxomatosis.[11]

Vaccination against myxomatosis is currently prohibited in Australia due to concerns that the vaccine virus could spread to wild rabbits and increase their immunity to myxomatosis. As feral rabbits in Australia already cause a great deal of environmental damage, this concern is taken seriously by the government.[12] Many pet rabbits in Australia continue to die from myxomatosis due to their lack of immunity.[13] There is at least one campaign to allow the vaccine for domestic pets.[14] The Australian Veterinary Association supports the introduction of a safe and effective myxomatosis vaccine for pet rabbits,[15] and the RSPCA of Australia has repeatedly called for a review of available myxoma virus vaccines and a scientific assessment of their likely impacts in the Australian setting.[16]

Although myxomatosis is endemic in parts of Mexico and the United States, there is no commercially available vaccine in either of these countries. Information on recently reported cases in the United States is available from the House Rabbit Society.[17] In the United States the importation of vaccines is overseen by the Animal and Plant Health Inspection Service, part of the Department of Agriculture.[18]

Other preventive measures

In locations where myxomatosis is endemic but no vaccine is available, preventing exposure to the myxoma virus is of vital importance. Even vaccinated rabbits need protection, as the vaccines are not 100% effective. The risk of a pet contracting myxomatosis can be reduced by preventing contact with wild rabbits, keeping rabbits indoors (preferred) or behind screens to prevent mosquito exposure, and using rabbit-safe medications to treat and prevent fleas, lice, and mites. Any new rabbit that may have been exposed should be quarantined, and rabbits suspected of having myxomatosis should be immediately isolated until the diagnosis is ruled out. If the disease is confirmed all contaminated cages, dishes, or other objects should be disinfected with 10% bleach, 10% sodium hydroxide, or 1%–1.4% formalin.[19]

Use as a population control agent
Rabbit and myxomatosis introductions around the world with dates

Myxoma virus was the first virus intentionally introduced into the wild with the purpose of eradicating a vertebrate pest, namely the European rabbit in Australia and Europe. The long-term failure of this strategy has been due to natural selective pressures on both the rabbit and virus populations, which resulted in the emergence of myxomatosis-resistant animals and attenuated virus variants. The process is regarded as a classical example of host–pathogen coevolution following cross-species transmission of a pathogen.[20]

Australia
Rabbits around a waterhole in the myxomatosis trial site on Wardang Island, Australia in 1938
Releasing the myxoma virus in Australia

European rabbits were brought to Australia in 1788 by early English settlers (see Rabbits in Australia). Initially used as a food source, they later became feral and their numbers soared. In November 1937, the Australian Council for Scientific and Industrial Research used Wardang Island to conduct its first field trials of myxomatosis, which established the methodology for the successful release of the myxoma virus throughout the country.[21]

In 1950, the SLS strain of myxoma virus from the South American tapeti (Sylvilagus brasiliensis) was released in Australia as a biological control agent against feral rabbits. The virus was at first highly lethal, with an estimated case fatality rate of close to 99.8%. Within a few years, however, this strain was replaced by less virulent ones, which permitted longer survival of infected rabbits and enhanced disease transmission. The virus created strong selection pressure for the evolution of rabbits resistant to myxomatosis. As rabbits became more resistant the viral strains responded by becoming less virulent.[2] Rabbit hemorrhagic disease virus has also been used to control wild rabbit populations in Australia since 1995.[22]

Europe

In June 1952, Paul-Félix Armand-Delille, the owner of an estate in northwestern France, inoculated two wild rabbits with the Lausanne strain of myxoma virus.[23] His intention was to only eradicate rabbits on his property, but the disease quickly spread through Western Europe, Ireland and the United Kingdom.[24] Some dissemination of the virus clearly appeared deliberate, such as the introduction into Britain in 1953 and the introduction into Ireland in 1954.[25] Unlike in Australia, however, strenuous efforts were made to stop the spread in Europe. These efforts proved in vain. According to estimates, the wild rabbit population in the United Kingdom fell by 99%, in France by 90% to 95%, and in Spain by 95%. This in turn drove specialized rabbit predators, such as the Iberian lynx and the Spanish imperial eagle, to the brink of extinction.[26][27]

Myxomatosis not only decreased the wild rabbit population and the population of its natural predators, but also had significant impacts on the large rabbit-farming industry, which produced domestic rabbits for meat and fur.[28] The Lausanne strain of the myxoma virus causes the formation of large purple skin-nodules, a sign not seen in other strains. As happened in Australia, the virus has generally become less virulent and the wild rabbit populations more resistant subsequently.[24]

New Zealand

The introduction of myxomatosis into New Zealand in 1952 to control a burgeoning rabbit problem failed for lack of a vector.[29]

South America

Two pairs of European rabbits set free in 1936 at Punta Santa Maria resulted in an infestation that spread over the northern half of Tierra del Fuego. More rabbits were introduced in 1950 near Ushuaia by the Argentinian Navy and a private rabbit farmer. The rabbits quickly became pests, riddling the ground with holes and leaving it bare of grass. By 1953 the rabbit population numbered about 30 million. In 1954 Chilean authorities introduced a Brazilian strain of myxoma virus to Tierra del Fuego, which succeeded in bringing rabbits to very low population levels.[30]

Use as an evolutionary model
Evolutionary history of the myxoma virus in Europe and Australia

Given the importance of viral evolution to disease emergence, pathogenesis, drug resistance, and vaccine efficacy, it has been well studied by theoreticians and experimentalists. The introductions of myxoma virus into European rabbit populations in Australia and France created natural experiments in virulence evolution.[31] While initial viral strains were highly virulent, attenuated strains were soon recovered from the field. These attenuated strains, which allowed rabbits to survive longer, came to dominate because they were more readily transmitted. As the complete genome sequences of multiple myxoma strains have been published, scientists have been able to pinpoint exactly which genes are responsible for the changes in the myxoma virus's virulence and behavior.[32]

However, the evolution of the disease has proved to be increasingly complex and unpredictable, both among various strains of host, and among strains of the virus. Both in modes of resistance and of virulence, and in all countries in which the virus has been introduced for control of feral rabbits, the hosts and the pathogens have continually adapted in various ways to evolutionary challenges. Although current strains of myxomatosis do not provide sufficient control on their own, the disease remains a significant ecological factor in rabbit control, both in Australia and in other countries. For example, in spite of long-term concern in Australia especially, where the initial virulence of myxomatosis declined after a few decades in the field, rabbit evolution of resistance to the disease has not gone unchallenged. For some recent strains of the virus, as a case in point, selection for reduced inflammation prolongs virus replication, which enhances transmission and reduces suppression of the virus by the host's fever; it also may cause immunosuppression, which favours high virulence.[33] Studies continue, both in the contexts of rabbit control, and of the relevant evolutionary principles.

In culture

Myxomatosis is referred to as "the white blindness" by the rabbit characters of the novel Watership Down (1972) by Richard Adams, and in the story a rabbit chief had driven out all rabbits who seemed to be afflicted. In one of the novel's folk tales about the rabbit hero El-ahrairah, the transmission of the disease is explained to him by the lord of the rabbit underworld, the Black Rabbit of Inle ("it is carried by the fleas in rabbits' ears; they pass from the ears of a sick rabbit to those of his companions").[34]

The sixth album of british band Radiohead "Hail to the Thief" contains a song "Myxomatosis". The disease is used as analogy to the journalist attention the band has. Thom Yorke has said:

"I remember my parents pointing out all these dead rabbits on the road when I was a kid. I didn’t know that much about the virus, or even how to spell it, but I loved the word. I loved the way it sounded." [35]

References
  1. Meredith, A (2013). "Viral skin diseases of the rabbit". Veterinary Clinics of North America: Exotic Animal Practice. 16 (3): 705–714. doi:10.1016/j.cvex.2013.05.010. PMID 24018033.
  2. Kerr, P (2017). "Genomic and phenotypic characterization of myxoma virus from Great Britain reveals multiple evolutionary pathways distinct from those in Australia". PLOS Pathogens. 13 (3): e1006252. doi:10.1371/journal.ppat.1006252. PMC 5349684. PMID 28253375.
  3. Kerr, P (2013). "Viral Infections of Rabbits". Veterinary Clinics of North America: Exotic Animal Practice. 16 (2): 437–468. doi:10.1016/j.cvex.2013.02.002. PMC 7110462. PMID 23642871.
  4. "Disinfection". The Center for Food Security and Public Health. Retrieved 21 July 2019.
  5. Fenner, F; Woodroofe, GM (1953). "The pathogenesis of infectious myxomatosis: the mechanism of infection and the immunological response in the European rabbit (Oryctolagus cuniculus)". The British Journal of Experimental Pathology. 34 (4): 400–411. PMC 2073564. PMID 13093911.
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  23. Davis, J. "Darwin's rabbit is revealing how the animals became immune to myxomatosis". Natural History Museum. Retrieved 14 August 2019.
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Further reading
  • Deane, C.D. (1955). "Note on myxomatosis in hares". Bulletin of the Mammal Society of the British Isles. 3: 20. OCLC 1224626693.
  • Fenner, Frank; Ratcliffe, F.N. (1965). Myxomatosis. Cambridge University Press. ISBN 978-0521049917.
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