Myoblast city

Myoblast city (Mbc[1]) is the Drosophila melanogaster ortholog of the mammalian protein Dock180.[2] Mutant mbc embryos exhibit defects in dorsal closure, cytoskeletal organization, myogenesis, and neural development.[2][3]

Myoblast city
Identifiers
OrganismDrosophila melanogaster
Symbolmbc
UniProtQ9VCH4
Search for
StructuresSwiss-model
DomainsInterPro

Discovery

The Myoblast city locus was identified by deletion mapping, using this technique researchers were able to isolate the location of the gene on the right arm of the third chromosome.[4] During the process four recessive alleles of Mbc were found; mbcc1, mbcc2, mbcc3, mbcs4, all of which are lethal and the D. melanogaster embryos fail to hatch.[4]

During the first 9-10 hours of development, embryos with the mutant Mbc alleles show the same myosin expression as wild-type embryos.[4] However, at about the 11th hour, most myoblasts fail to fuse. As development progresses, some myoblasts show signs of fusion, such as elongation and having multiple nuclei; but some remain round.[4] After 13-14 hours of development, most cells in mutant embryos that failed to fuse lose myosin expression.[4]

References
  1. "FlyBase Gene Report:Dmel\mbc". FlyBase Consortium. Retrieved February 25, 2009.
  2. Erickson MR, Galletta BJ, Abmayr SM (August 1997). "Drosophila myoblast city encodes a conserved protein that is essential for myoblast fusion, dorsal closure, and cytoskeletal organization". J. Cell Biol. 138 (3): 589–603. doi:10.1083/jcb.138.3.589. PMC 2141626. PMID 9245788.
  3. Nolan KM, Barrett K, Lu Y, Hu KQ, Vincent S, Settleman J (November 1998). "Myoblast city, the Drosophila homolog of DOCK180/CED-5, is required in a Rac signaling pathway utilized for multiple developmental processes". Genes Dev. 12 (21): 3337–42. doi:10.1101/gad.12.21.3337. PMC 317223. PMID 9808621.
  4. Rushton, E.; Drysdale, R.; Abmayr, S.M.; Michelson, A.M.; Bate, M. (1995-07-01). "Mutations in a novel gene, myoblast city, provide evidence in support of the founder cell hypothesis for Drosophila muscle development". Development. 121 (7): 1979–1988. doi:10.1242/dev.121.7.1979. ISSN 1477-9129. PMID 7635046.


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