NEK9

NEK9
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	3ZKE, 3ZKF
Identifiers
Aliases	NEK9, NERCC, NERCC1, Nek8, NIMA related kinase 9, APUG, LCCS10, NC
External IDs	OMIM: 609798 MGI: 2387995 HomoloGene: 13222 GeneCards: NEK9
Gene location (Human)
Chr.	Chromosome 14 (human)[1]
End	75,127,344 bp[1]
Gene location (Mouse)
Chr.	Chromosome 12 (mouse)[2]
End	85,386,136 bp[2]
RNA expression pattern
	Top expressed in
	tibia; ; right uterine tube; ; canal of the cervix; ; gastrocnemius muscle; ; stromal cell of endometrium; ; gastric mucosa; ; germinal epithelium; ; left uterine tube; ; tibial nerve; ; right coronary artery;
	Top expressed in
	vastus lateralis muscle; ; iris; ; triceps brachii muscle; ; sternocleidomastoid muscle; ; utricle; ; temporal muscle; ; ureter; ; endocardial cushion; ; medullary collecting duct; ; digastric muscle;
	More reference expression data
	; ;
	More reference expression data
Gene ontology
Molecular function	transferase activity; nucleotide binding; protein kinase activity; metal ion binding; kinase activity; protein binding; ATP binding; protein kinase binding; protein serine/threonine kinase activity;
Cellular component	cytosol; nucleus; cytoplasm; centrosome;
Biological process	phosphorylation; cell division; mitotic nuclear membrane disassembly; cell cycle; protein phosphorylation;
	Sources:Amigo / QuickGO
Orthologs
	91754
	217718
	ENSG00000119638
	ENSMUSG00000034290
	Q8TD19
	Q8K1R7
	NM_033116; NM_001329237; NM_001329238
	NM_145138
	NP_001316166; NP_001316167; NP_149107
	NP_660120
	Wikidata
View/Edit Human	View/Edit Mouse

Serine/threonine-protein kinase Nek9 is an enzyme that in humans is encoded by the NEK9 gene.[5][6]

Interactions

NEK9 has been shown to interact with:

Model organisms

Model organisms have been used in the study of NEK9 function. A conditional knockout mouse line called Nek9^{tm1a(EUCOMM)Wtsi} was generated at the Wellcome Trust Sanger Institute.[11] Male and female animals underwent a standardized phenotypic screen[12] to determine the effects of deletion.[13][14][15][16] Additional screens performed: - In-depth immunological phenotyping[17]

*Nek9* knockout mouse phenotype
Characteristic	Phenotype
All data available at.[12][17]	Peripheral blood leukocytes 6 Weeks	Normal
Insulin	Normal
Haematology 6 Weeks	Normal
Homozygous viability at P14	Abnormal
Recessive lethal study	Abnormal
Body weight	Normal
Neurological assessment	Normal
Grip strength	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology 16 Weeks	Normal
Peripheral blood leukocytes 16 Weeks	Normal
Heart weight	Normal
Salmonella infection	Normal
Cytotoxic T Cell Function	Normal
Spleen Immunophenotyping	Normal
Mesenteric Lymph Node Immunophenotyping	Normal
Bone Marrow Immunophenotyping	Normal
Epidermal Immune Composition	Normal

References

GRCh38: Ensembl release 89: ENSG00000119638 - Ensembl, May 2017
GRCm38: Ensembl release 89: ENSMUSG00000034290 - Ensembl, May 2017
"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
Holland PM, Milne A, Garka K, Johnson RS, Willis C, Sims JE, Rauch CT, Bird TA, Virca GD (May 2002). "Purification, cloning, and characterization of Nek8, a novel NIMA-related kinase, and its candidate substrate Bicd2". The Journal of Biological Chemistry. 277 (18): 16229–40. doi:10.1074/jbc.M108662200. PMID 11864968.
"Entrez Gene: NEK9 NIMA (never in mitosis gene a)- related kinase 9".
Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3: 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
Belham C, Roig J, Caldwell JA, Aoyama Y, Kemp BE, Comb M, Avruch J (Sep 2003). "A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases". The Journal of Biological Chemistry. 278 (37): 34897–909. doi:10.1074/jbc.M303663200. PMID 12840024.
Roig J, Mikhailov A, Belham C, Avruch J (Jul 2002). "Nercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and regulates mitotic progression". Genes & Development. 16 (13): 1640–58. doi:10.1101/gad.972202. PMC 186374. PMID 12101123.
Tan BC, Lee SC (Mar 2004). "Nek9, a novel FACT-associated protein, modulates interphase progression". The Journal of Biological Chemistry. 279 (10): 9321–30. doi:10.1074/jbc.M311477200. PMID 14660563.
Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.
"International Mouse Phenotyping Consortium".
Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
"Infection and Immunity Immunophenotyping (3i) Consortium".

Pelka P, Scimè A, Mandalfino C, Joch M, Abdulla P, Whyte P (Jul 2007). "Adenovirus E1A proteins direct subcellular redistribution of Nek9, a NimA-related kinase". Journal of Cellular Physiology. 212 (1): 13–25. doi:10.1002/jcp.20983. PMID 17443675. S2CID 41137896.
Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
Wissing J, Jänsch L, Nimtz M, Dieterich G, Hornberger R, Kéri G, Wehland J, Daub H (Mar 2007). "Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry" (PDF). Molecular & Cellular Proteomics. 6 (3): 537–47. doi:10.1074/mcp.T600062-MCP200. PMID 17192257.
Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (Nov 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983.
Bowers AJ, Boylan JF (Mar 2004). "Nek8, a NIMA family kinase member, is overexpressed in primary human breast tumors". Gene. 328: 135–42. doi:10.1016/j.gene.2003.12.002. PMID 15019993.
Tan BC, Lee SC (Mar 2004). "Nek9, a novel FACT-associated protein, modulates interphase progression". The Journal of Biological Chemistry. 279 (10): 9321–30. doi:10.1074/jbc.M311477200. PMID 14660563.
Belham C, Roig J, Caldwell JA, Aoyama Y, Kemp BE, Comb M, Avruch J (Sep 2003). "A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases". The Journal of Biological Chemistry. 278 (37): 34897–909. doi:10.1074/jbc.M303663200. PMID 12840024.
Roig J, Mikhailov A, Belham C, Avruch J (Jul 2002). "Nercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and regulates mitotic progression". Genes & Development. 16 (13): 1640–58. doi:10.1101/gad.972202. PMC 186374. PMID 12101123.
Ohara O, Nagase T, Mitsui G, Kohga H, Kikuno R, Hiraoka S, Takahashi Y, Kitajima S, Saga Y, Koseki H (Apr 2002). "Characterization of size-fractionated cDNA libraries generated by the in vitro recombination-assisted method". DNA Research. 9 (2): 47–57. doi:10.1093/dnares/9.2.47. PMID 12056414.

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[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000119638 - Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000034290 - Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid11864968-5] Holland PM, Milne A, Garka K, Johnson RS, Willis C, Sims JE, Rauch CT, Bird TA, Virca GD (May 2002). "Purification, cloning, and characterization of Nek8, a novel NIMA-related kinase, and its candidate substrate Bicd2". The Journal of Biological Chemistry. 277 (18): 16229–40. doi:10.1074/jbc.M108662200. PMID 11864968.

[entrez-6] "Entrez Gene: NEK9 NIMA (never in mitosis gene a)- related kinase 9".

[pmid17353931-7] Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3: 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.

[pmid12840024-8] Belham C, Roig J, Caldwell JA, Aoyama Y, Kemp BE, Comb M, Avruch J (Sep 2003). "A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases". The Journal of Biological Chemistry. 278 (37): 34897–909. doi:10.1074/jbc.M303663200. PMID 12840024.

[pmid12101123-9] Roig J, Mikhailov A, Belham C, Avruch J (Jul 2002). "Nercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and regulates mitotic progression". Genes & Development. 16 (13): 1640–58. doi:10.1101/gad.972202. PMC 186374. PMID 12101123.

[pmid14660563-10] Tan BC, Lee SC (Mar 2004). "Nek9, a novel FACT-associated protein, modulates interphase progression". The Journal of Biological Chemistry. 279 (10): 9321–30. doi:10.1074/jbc.M311477200. PMID 14660563.

[mgp_reference-11] Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.

[IMPCsearch_ref-12] "International Mouse Phenotyping Consortium".

[pmid21677750-13] Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.

[mouse_library-14] Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.

[mouse_for_all_reasons-15] Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.

[pmid23870131-16] White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.

[iii_ref-17] "Infection and Immunity Immunophenotyping (3i) Consortium".

NEK9

Interactions

Model organisms

References

Further reading