Nephridiophaga

Nephridiophaga is a genus of single cell eukaryotes, now considered to be fungi, that pathogenically inhabit the Malpighian tubules of insects.[1][2] Ivanic described the type species from honey bees.[2]

Nephridiophaga
Nephridiophaga blattellae, 10–13 transmission electron microscopy, 14 Calcofluor white staining. 10 Meront with several nuclei (n) and mitochondria (mi) in the lumen of Malpighian tubule. Inset: Mitochondrium with tubular to sac-like cristae. 11 Sporogenic plasmodium containing mature spores (sp), mitochondria (mi), and vegetative nuclei (n) in the cytoplasm. The plasmodium is anchored to the microvilli (mv) of epithelial cells (ep) of the tubule. 12 Young spore within the cytoplasm of a sporogenic plasmodium, surrounded by a layer of vesicles. The spore cytoplasm contains one nucleus (n), mitochondria (mi), and endoplasmic reticulum (er). 13 An infectious sporoplasm hatches through the central spore opening, leaving behind the spore wall of the emptying spore (sp). The nucleus (n) is squeezed through the tiny spore opening. 14 Calcofluor white stains the spore wall indicating the presence of chitin (bluish color). Scale bars: 1 µm (10–13), inset 0.1 µm (10), 5 µm (14)
Scientific classification
Kingdom:
Fungi
Division:
Chytridiomycota
Class:
incertae sedis
Order:
Nephridiophagales
Family:
incertae sedis
Genus:
Nephridiophaga

Ivanić 1937
Type species
Nepharidiophaga apis
Ivanic 1937
Synonyms
  • Coelosporidium Mesnil & Marchoux 1897

Morphology and life cycle

Nephridiophaga spores are small, flattened ovals that resemble a red blood cell. Each spore contains a single nucleus and may have an inconspicuous, central opening. Spores are ingested by insects. Once inside the host, spores germinate, though whether in the gut or elsewhere is not known. Plasmodia inhabit and feed on Malpighian tubule cells where they are in direct contact with the cytoplasm. Multi-nucleate plasmodia are eventually released into the lumen, some attach to the brush border of the tubule cells using pseudopodia that interdigitate with the microvilli of the cells. Other plasmodia form merozoite-like cells. Plasmodia released into the lumen form sporoblasts internally with a spore wall enclosing each nucleus. Sporoblasts can be asexual or sexual. Asexual sporoblasts are formed from a type of closed mitosis with chromosomes un-condensed and spindle poles remaining un-connected to the nuclear envelope, which is a common form of mitosis for fungi. Sexual sporoblasts are formed after the fusion of meiotic nuclei; however, the details of meiosis and fusion are not known, and the phenomenon is doubted by some authors.[1][3][4] Spores are excreted from the insect in its feces.[1]

Taxonomy

Nephridiophaga was originally placed in the protist order Haplosporidia, though not all researchers agreed, with some placing it in the Microsporidia, and others leaving it unclassified. Molecular phylogenetic studies of species infecting cockroaches have shown that the genus belongs in or close to the Chytrid division of the kingdom Fungi.[2][5]

Species

Based on[4][6][7][2]

  • Nephridiophaga apis Ivanić 1937
  • Nephridiophaga archimandrita Radek, Wellmanns & Wolf 2011
  • Nephridiophaga blaberi Radek & Storch 2000
  • Nephridiophaga blatellae (Crawley 1905) Woolever 1966
  • Nephridiophaga forficulae (Léger 1909) Ormières & Manier 1973
  • Nephridiophaga lucihormetica Radek, Wellmanns & Wolf 2011
  • Nephridiophaga maderae Radek et al. 2017
  • Nephridiophaga meloidorum (Purrini & Rohde 1988) Lange 1993
  • Nephridiophaga ormieresi Toguebaye et al. 1986
  • Nephridiophaga periplanetae (Lutz & Splendore 1903) Lange 1933
  • Nephridiophaga schalleri (Pumni & Rohde 1988) Lange 1993
  • Nephridiophaga tangae (Purrini, Weiser & Kohring 1988) Lange 1993
  • Nephridiophaga xenoboli Ganapati & Narasimhamurti 1960

References
  1. Woolever, Patricia (1966). "Life history and electron microscopy of a haplosporidian, Nephridiophaga blattellae (Crawley) n. comb., in the Malphigian tubulues of the German cockroach, Blattella germanica (L.)". Journal of Protozoology. 13 (4): 622–642. doi:10.1111/j.1550-7408.1966.tb01973.x.
  2. Radek, Renate; Wurzbacher, Christian; Gisder, Sebastian; Nilsson, R. Henrick; Owerfeldt, Anja; Genersch, Elke; Kirk, Paul K.; Voigt, Kerstin (2017). "Morphologic and molecular data help adopting the insect-pathogenic nephridiophagids (Nephridiophagidae) among the early diverging fungal lineages, close to the Chytridiomycota" (PDF). MycoKeys. 25: 31–50. doi:10.3897/mycokeys.25.12446.
  3. Lange, Carlos E. (1993). "Unclassified protists of arthropods: The ultrastructure of Nephridiophaga perilanetae (Lutz & Splendore, 1903) N. Comb., and the affinities of the Nephridiophagidae to other protists". Journal of Eukaryotic Microbiology. 40 (6): 689–700. doi:10.1111/j.1550-7408.1993.tb04461.x. S2CID 83887071.
  4. Radek, R.; Herth, W. (1999). "Ultrastructural investigation of the spore-forming protist Nephridiophaga blattellae in the Malpighian tubules of the German cockroah Blattella germanica". Parasitology Research. 85 (3): 216–231. doi:10.1007/s004360050538. PMID 9951966. S2CID 24356869.
  5. "the Nephridiophaga page". Species Fungorum. Royal Botanic Gardens Kew. Retrieved 25 May 2021.
  6. Fabel, Paola; Radek, Renate; Storch, Volker (2000). "A new spore-forming protist, Nephridiophaga blaberi sp. nov., in the death's head cockroch Blaberus craniifer". European Journal of Protistology. 36 (4): 387–395. doi:10.1016/S0932-4739(00)80044-9.
  7. Radek, Ranate; Wellmanns, Daniel; Wolf, Anja (2011). "Two new species of Nephridiphaga (Zygomycota) in the Malpighian tubules of cockroaches". Parasitology Research. 109 (2): 473–482. doi:10.1007/s00436-011-2278-7. PMID 21347521. S2CID 6925763.
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