Norman Horowitz

Horowitz earned his BS in biology at the University of Pittsburgh in 1936 where his experience conducting research as an undergraduate helped to persuade him to pursue further graduate training in science. He later established the endowed Norman H. Horowitz Fellowship at the University of Pittsburgh to support undergraduate research.[4] He completed his PhD at Caltech in 1939 under embryologist Albert Tyler, and then became a postdoctoral researcher at Stanford University in the laboratory of George W. Beadle.[5] Horowitz returned to Caltech as a faculty member in 1946 and stayed at the Institute for the remainder of his career. He served as chair of the Biology Division from 1977 to 1980, and became professor emeritus in 1982.[6]

As a scientist, Horowitz is best known for his discovery and demonstration in 1944 that a metabolic pathway is a series of steps, each catalyzed by a single enzyme. Working with Neurospora crassa, Horowitz demonstrated that each step in the metabolism of arginine from its precursors depends on the intactness of a single gene. His discovery helped to clinch the case for George Beadle and Edward Tatum's "one gene-one enzyme hypothesis" (a term Horowitz coined for their concept).

The importance of the “one gene-one enzyme” concept can only be understood in the context of how geneticists thought about the gene in the first half of the twentieth century. Many geneticists doubted that genes, as definable entities even existed.[2] Rather they attributed the phenotypes of mutants to altered properties of whole chromosomes. Even among geneticists who believed in the existence of individual genes, a vague concern was often expressed that there was no simple causative relationship between genes and proteins. Metzenberg[2] remarked on the stubborn courage required for Horowitz to espouse and defend the initially unpopular idea of “one gene-one enzyme” and to see it through to general acceptance.

Another important contribution of Horowitz was his 1945 proposal on the “backward evolution” of biosynthetic pathways.[7] This proposal provided a framework for understanding the evolution of biosynthetic pathways and presaged the study of molecular evolution. Horowitz proposed that the earliest life forms reproduced themselves by utilizing non-biologic organic molecules in the environment. Depletion of such organic molecules by the early reproducing life forms could have continued until a point was reached where the supply of one of these molecules limited further multiplication. By a process of mutation and natural selection, these life forms then evolved a catalytic activity for utilizing another related available molecule by catalyzing its conversion to the previously limiting molecule. When this precursor molecule was in turn eventually depleted, further mutations allowed the conversion of another available substrate (a pre- precursor) into the precursor. Horowitz considered that repetition of such mutation/selection events formed the basis of the evolution of biosynthetic pathways.

Horowitz was head of the Pyrolytic Release experiment during the Viking program.[8]

Dr Beadle (1958 Nobel Prize winner in Medicine) gave credit to Dr. Horowitz for his original work on biological reactions.[9]

The results from incubating Martian soil with complex media, and from pyrolysis of soil followed by gas chromatography and mass spectrometry of the pyrolysis products, suggested that the surface of Mars is lifeless.[2] Furthermore, in the soil samples analyzed by the Viking program there were no detectable biochemical footprints in the form of organic compounds that might be expected if living organisms ever thrived on Mars. However, in 2013, NASA’s Curiosity rover provided evidence of an ancient Martian lake (in existence about 3.7 billion years ago) that may have been capable of supporting life for long stretches --- perhaps for millions of years.[10] Thus, although Horowitz’s pyrolytic release experiments in 1976 provided the first indication that there is no current life on the surface of Mars, it remains possible that life did exist on Mars in the distant past.

• One gene-one enzyme hypothesis

• Anderson, Charlene. In Memoriam: Norman Horowitz (1915-2005) The Planetary Society.
• Horowitz, N. H. (1945). "On the Evolution of Biochemical Syntheses". Proceedings of the National Academy of Sciences of the United States of America. 31 (6): 153–157. Bibcode:1945PNAS...31..153H. doi:10.1073/pnas.31.6.153. PMC 1078786. PMID 16578152.
• Horowitz, Norman Harold (1986). To utopia and back: the search for life in the solar system. San Francisco: W.H. Freeman. ISBN 978-0-7167-1766-9.

• National Academy of Sciences Biographical Memoir