POLR3G

POLR3G
Identifiers
Aliases	POLR3G, RPC32, RPC7, polymerase (RNA) III subunit G, RNA polymerase III subunit G, C31
External IDs	OMIM: 617456 MGI: 1914736 HomoloGene: 38184 GeneCards: POLR3G
Gene location (Human)
Chr.	Chromosome 5 (human)[1]
End	90,514,557 bp[1]
Gene location (Mouse)
Chr.	Chromosome 13 (mouse)[2]
End	81,859,132 bp[2]
RNA expression pattern
	Top expressed in
	spinal ganglia; ; sural nerve; ; secondary oocyte; ; internal globus pallidus; ; monocyte; ; trigeminal ganglion; ; cerebellar hemisphere; ; substantia nigra; ; pancreatic ductal cell; ; stromal cell of endometrium;
	Top expressed in
	morula; ; primitive streak; ; esophagus; ; facial motor nucleus; ; yolk sac; ; urothelium; ; epidermis; ; lip; ; hair follicle; ; renal corpuscle;
	More reference expression data
	n/a
Gene ontology
Molecular function	DNA-directed 5'-3' RNA polymerase activity; protein binding;
Cellular component	RNA polymerase III complex; cytosol; nucleoplasm; nucleus; cytoplasm;
Biological process	positive regulation of interferon-beta production; immune system process; cell population proliferation; defense response to virus; positive regulation of innate immune response; innate immune response; regulation of transcription by RNA polymerase III; transcription by RNA polymerase III; positive regulation of type I interferon production;
	Sources:Amigo / QuickGO
Orthologs
	10622
	67486
	ENSG00000113356
	ENSMUSG00000035834
	O15318
	Q6NXY9
	NM_006467; NM_001370351; NM_001370352; NM_001370353; NM_001370354
	NM_001081176; NM_026190
	NP_006458; NP_001357280; NP_001357281; NP_001357282; NP_001357283
	NP_001074645
	Wikidata
View/Edit Human	View/Edit Mouse

Polymerase (RNA) III (DNA directed) polypeptide G (32kD) is a protein that in humans is encoded by the POLR3G gene. [5]

Model organisms

Model organisms have been used in the study of POLR3G function. A conditional knockout mouse line called Polr3g^{tm1a(EUCOMM)Wtsi} was generated at the Wellcome Trust Sanger Institute.[6] Male and female animals underwent a standardized phenotypic screen[7] to determine the effects of deletion.[8][9][10][11] Additional screens performed: - In-depth immunological phenotyping[12]

*Polr3g* knockout mouse phenotype
Characteristic	Phenotype
All data available at.[7][12]
Insulin	Normal
Homozygous viability at P14	Abnormal
Recessive lethal study	Abnormal
Body weight	Normal
Neurological assessment	Normal
Grip strength	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology 16 Weeks	Normal
Peripheral blood leukocytes 16 Weeks	Normal
Heart weight	Normal
Cytotoxic T Cell Function	Normal
Spleen Immunophenotyping	Normal
Mesenteric Lymph Node Immunophenotyping	Normal
Bone Marrow Immunophenotyping	Normal
Epidermal Immune Composition	Normal
Influenza Challenge	Normal

References

GRCh38: Ensembl release 89: ENSG00000113356 - Ensembl, May 2017
GRCm38: Ensembl release 89: ENSMUSG00000035834 - Ensembl, May 2017
"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
"Entrez Gene: Polymerase (RNA) III (DNA directed) polypeptide G (32kD)". Retrieved 2014-04-01.
Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.
"International Mouse Phenotyping Consortium".
Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. S2CID 18872015.
White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Sanger Institute Mouse Genetics Project, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
"Infection and Immunity Immunophenotyping (3i) Consortium".

Ablasser, A; Bauernfeind, F; Hartmann, G; Latz, E; Fitzgerald, K. A.; Hornung, V (2009). "RIG-I-dependent sensing of poly(dA:dT) through the induction of an RNA polymerase III-transcribed RNA intermediate" (PDF). Nature Immunology. 10 (10): 1065–72. doi:10.1038/ni.1779. PMC 3878616. PMID 19609254.
Chiu, Y. H.; MacMillan, J. B.; Chen, Z. J. (2009). "RNA polymerase III detects cytosolic DNA and induces type I interferons through the RIG-I pathway". Cell. 138 (3): 576–91. doi:10.1016/j.cell.2009.06.015. PMC 2747301. PMID 19631370.
Chantarangsu, S; Mushiroda, T; Mahasirimongkol, S; Kiertiburanakul, S; Sungkanuparph, S; Manosuthi, W; Tantisiriwat, W; Charoenyingwattana, A; Sura, T; Takahashi, A; Kubo, M; Kamatani, N; Chantratita, W; Nakamura, Y (2011). "Genome-wide association study identifies variations in 6p21.3 associated with nevirapine-induced rash". Clinical Infectious Diseases. 53 (4): 341–8. doi:10.1093/cid/cir403. PMID 21810746.
Wong, R. C.; Pollan, S; Fong, H; Ibrahim, A; Smith, E. L.; Ho, M; Laslett, A. L.; Donovan, P. J. (2011). "A novel role for an RNA polymerase III subunit POLR3G in regulating pluripotency in human embryonic stem cells". Stem Cells. 29 (10): 1517–27. doi:10.1002/stem.714. PMID 21898682. S2CID 24305950.
Jang, K. L.; Collins, M. K.; Latchman, D. S. (1992). "The human immunodeficiency virus tat protein increases the transcription of human Alu repeated sequences by increasing the activity of the cellular transcription factor TFIIIC". Journal of Acquired Immune Deficiency Syndromes. 5 (11): 1142–7. PMID 1403646.
Wang, Z; Roeder, R. G. (1997). "Three human RNA polymerase III-specific subunits form a subcomplex with a selective function in specific transcription initiation". Genes & Development. 11 (10): 1315–26. doi:10.1101/gad.11.10.1315. PMID 9171375.
Hu, P; Wu, S; Sun, Y; Yuan, C. C.; Kobayashi, R; Myers, M. P.; Hernandez, N (2002). "Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits". Molecular and Cellular Biology. 22 (22): 8044–55. doi:10.1128/MCB.22.22.8044-8055.2002. PMC 134740. PMID 12391170.

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[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000113356 - Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000035834 - Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[entrez-5] "Entrez Gene: Polymerase (RNA) III (DNA directed) polypeptide G (32kD)". Retrieved 2014-04-01.

[mgp_reference-6] Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.

[IMPCsearch_ref-7] "International Mouse Phenotyping Consortium".

[pmid21677750-8] Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.

[mouse_library-9] Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.

[mouse_for_all_reasons-10] Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. S2CID 18872015.

[pmid23870131-11] White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Sanger Institute Mouse Genetics Project, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.

[iii_ref-12] "Infection and Immunity Immunophenotyping (3i) Consortium".

POLR3G

Model organisms

References

Further reading