PRMT8

Protein arginine methyltransferase 8 is a protein that in humans is encoded by the PRMT8 gene.[1] Arginine methylation is a posttranslational modification involved in a number of cellular processes, including DNA repair, RNA transcription, signal transduction and protein compartmentalization.[1] PRMT8 binds and dimethylates Ewing sarcoma breakpoint region 1 (EWS) protein.[2]

Model organisms

Model organisms have been used in the study of PRMT8 function. A conditional knockout mouse line, called Prmt8tm1a(EUCOMM)Wtsi[7][8] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.[9][10][11]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[5][12] Twenty four tests were carried out on homozygous mutant mice and one significant abnormality was observed: the animals had decreased IgG1 levels.[5]

References
  1. "Protein arginine methyltransferase 8". Retrieved 2011-12-04.
  2. Kim, J. D.; Kako, K.; Kakiuchi, M.; Park, G. G.; Fukamizu, A. (2008). "EWS is a substrate of type I protein arginine methyltransferase, PRMT8". International Journal of Molecular Medicine. 22 (3): 309–315. PMID 18698489.
  3. "Salmonella infection data for Prmt8". Wellcome Trust Sanger Institute.
  4. "Citrobacter infection data for Prmt8". Wellcome Trust Sanger Institute.
  5. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.
  6. Mouse Resources Portal, Wellcome Trust Sanger Institute.
  7. "International Knockout Mouse Consortium".
  8. "Mouse Genome Informatics".
  9. Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–342. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
  10. Dolgin E (2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
  11. Collins FS, Rossant J, Wurst W (2007). "A Mouse for All Reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. S2CID 18872015.
  12. van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biol. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.

Further reading


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