Polyethylenimine

PEI has a number of uses in laboratory biology, especially tissue culture, but is also toxic to cells if used in excess.[13][14] Toxicity is by two different mechanisms,[15] the disruption of the cell membrane leading to necrotic cell death (immediate) and disruption of the mitochondrial membrane after internalisation leading to apoptosis (delayed).

Polyethyleneimines are used in the cell culture of weakly anchoring cells to increase attachment. PEI is a cationic polymer; the negatively charged outer surfaces of cells are attracted to dishes coated in PEI, facilitating stronger attachments between the cells and the plate.

Poly(ethylenimine) was the second polymeric transfection agent discovered,[16] after poly-L-lysine. PEI condenses DNA into positively charged particles, which bind to anionic cell surface residues and are brought into the cell via endocytosis. Once inside the cell, protonation of the amines results in an influx of counter-ions and a lowering of the osmotic potential. Osmotic swelling results and bursts the vesicle releasing the polymer-DNA complex (polyplex) into the cytoplasm. If the polyplex unpacks then the DNA is free to diffuse to the nucleus.[17][18]

Poly(ethylenimine) is also an effective permeabilizer of the outer membrane of Gram-negative bacteria.[19]

Both linear and branched polyethylenimine have been used for CO₂ capture, frequently impregnated over porous materials. First use of PEI polymer in CO₂ capture was devoted to improve the CO₂ removal in space craft applications, impregnated over a polymeric matrix.[20] After that, the support was changed to MCM-41, an hexagonal mesostructured silica, and large amounts of PEI were retained in the so-called "molecular basket".[21] MCM-41-PEI adsorbent materials led to higher CO₂ adsorption capacities than bulk PEI or MCM-41 material individually considered. The authors claim that, in this case, a synergic effect takes place due to the high PEI dispersion inside the pore structure of the material. As a result of this improvement, further works were developed to study more in depth the behaviour of these materials. Exhaustive works have been focused on the CO₂ adsorption capacity as well as the CO₂/O₂ and CO₂/N₂ adsorption selectivity of several MCM-41-PEI materials with PEI polymers.[22][23] Also, PEI impregnation has been tested over different supports such as a glass fiber matrix [24] and monoliths.[25] However, for an appropriate performance under real conditions in post-combustion capture (mild temperatures between 45-75 °C and the presence of moisture) it is necessary to use thermally and hydrothermally stable silica materials, such as SBA-15,[26] which also presents an hexagonal mesostructure. Moisture and real world conditions have also been tested when using PEI-impregnated materials to adsorb CO₂ from the air.[27]

A detailed comparison among PEI and other amino-containing molecules showed an excellent performance of PEI-containing samples with cycles. Also, only a slight decrease was registered in their CO₂ uptake when increasing the temperature from 25 to 100 °C, demonstrating a high contribution of chemisorption to the adsorption capacity of these solids. For the same reason, the adsorption capacity under diluted CO₂ was up to 90% of the value under pure CO₂ and also, a high unwanted selectivity towards SO₂ was observed.[28] Lately, many efforts have been made in order to improve PEI diffusion within the porous structure of the support used. A better dispersion of PEI and a higher CO₂ efficiency (CO₂/NH molar ratio) were achieved by impregnating a template-occluded PE-MCM-41 material rather than perfect cylindrical pores of a calcined material,[29] following a previously described route.[30] The combined use of organosilanes such as aminopropyl-trimethoxysilane, AP, and PEI has also been studied. The first approach used a combination of them to impregnate porous supports, achieving faster CO₂-adsorption kinetics and higher stability during reutilization cycles, but no higher efficiencies.[31] A novel method is the so-called "double-functionalization". It is based on the impregnation of materials previously functionalized by grafting (covalent bonding of organosilanes). Amino groups incorporated by both paths have shown synergic effects, achieving high CO₂ uptakes up to 235 mg CO₂/g (5.34 mmol CO₂/g).[32] CO₂ adsorption kinetics were also studied for these materials, showing similar adsorption rates as impregnated solids.[33] This is an interesting finding, taking into account the smaller pore volume available in double-functionalized materials. Thus, it can be also concluded that their higher CO₂ uptake and efficiency compared to impregnated solids can be ascribed to a synergic effect of the amino groups incorporated by two methods (grafting and impregnation) rather than to a faster adsorption kinetics.

Poly(ethylenimine) and poly(ethylenimine) ethoxylated (PEIE) have been shown as effective low-work function modifiers for organic electronics by Zhou and Kippelen et al.[34] It could universally reduce the work function of metals, metal oxides, conducting polymers and graphene, and so on. It is very important that low-work function solution-processed conducting polymer could be produced by the PEI or PEIE modification. Based on this discovery, the polymers have been widely used for organic solar cells, organic light-emitting diodes, organic field-effect transistors, perovskite solar cells, perovskite light-emitting diodes, quantum-dot solar cells and light-emitting diodes etc.

Polyethylenimine (PEI), a cationic polymer, has been widely studied and shown great promise as an efficient gene delivery vehicle. Likewise, the HIV-1 Tat peptide, a cell-permeable peptide, has been successfully used for intracellular gene delivery.[35]