European corn borer

The European corn borer is about 1 inch (2.5 cm) long with a 0.75- to 1-inch (1.9–2.5 cm) wingspan. The female is light yellowish brown with dark, irregular, wavy bands across the wings. The male is slightly smaller and darker. The tip of its abdomen protrudes beyond its closed wings. They are most active before dawn. The adults spend most of their time feeding and mating. Males and females of different strains have been found to produce differing sex pheromones.[7]

Caterpillar

The fully grown larva is 0.75 to 1 inch (1.9–2.5 cm) in length. Larvae vary in color from light brown to pinkish gray and have conspicuous small, round, brown spots on each segment along the body. As they grow they reach between 2 and 20 mm. The larvae feed on the corn whorl and burrow into the stalk and ear. They have high mortality directly after emergence, but as soon as a feeding site is established, they have better survival rates. Total development before pupation lasts 50 days on average.[8]

Diapause, also known as hibernation, is induced in European corn borers by changes in temperature and daylight length. At higher temperatures, shorter photoperiods are sufficient to induce diapause. At 13.5 hours of light followed by 10.5 hours of dark, 100% of European corn borer larvae entered diapause regardless of temperature with the range of 18 to 29 °C (64 to 84 °F). At high temperatures and long photoperiods, fewer larvae enter diapause.[9]

Female corn borer moths lay clusters of eggs on corn leaves, usually on the undersides. The egg masses, or clusters, are laid in an overlapping configuration and are whitish yellow. As the larvae develop inside their eggs, the eggs become more and more transparent and the black heads of the immature caterpillars become visible. The caterpillars hatch by chewing their way out of the eggs.

A female moth can lay two egg masses per night over 10 nights. The number of eggs per egg mass decreases each day. The female lays white eggs which become pale yellow and finally translucent before hatching. The eggs hatch within three to seven days of laying.[10]

The original European corn borers introduced to North America in the early 20th century established a population in New York. This population produced one brood per year. A second population was introduced in Massachusetts and spread to Long Island and the Hudson River Valley. This second population produces two broods per year.[11]

If presented with the opportunity, female European corn borers, like most moths, mate with multiple males in a reproductive strategy known as polyandry. Polyandry confers several benefits to the females. For example, multiple matings increase female fecundity and longevity, because female moths receive both nutritional resources and multiple spermatophores from males. Furthermore, mating with multiple males ensures that the female receives enough sperm to completely fertilize her eggs. Additionally, it increases the reproductive fitness of females, because it increases the genetic diversity of the female's offspring - thus increasing the likelihood that they will mate and pass on her genes.[12]

Female calling behavior in European corn borers involves the extrusion of the pheromone gland and release of sex pheromones. This calling behavior is influenced by the moth's circadian rhythm and tends to occur at night. Higher humidity also induces the calling behavior, while desiccation, or drying out, decreases the calling behavior.[13] Both male and female European corn borers produce sex pheromones.[14]

There are two strains of European corn borers that are defined by their sex pheromone communication variant. These are the Z and E strains, named after the stereochemistry of the predominant isomer of 11-tetradecenyl acetate that they produce.[11] The E variant of pheromone has a trans- configuration of hydrogen molecules around its double bond, while the Z variant has a cis- configuration. The Z strain produces a 97:3 ratio of Z to E isomer pheromone while the E strain produces a 4:96 ratio of Z to E isomer pheromone. A mixture of isomers is much more efficient in attracting the moth than a single component.[15][11] The Z and E strains can mate and produce intermediate variants.[16]

Production of the specific pheromone blend in females is controlled by a single autosomal factor. Heterozygous females produce more E isomer than Z. The response to these pheromones in the olfactory cells of male European corn borers is also controlled by a single autosomal factor with two alleles. Analysis of the electrophysiological signaling of olfactory cells showed that those with two E alleles responded strongly to the E isomer and weakly to the Z isomer. The opposite effect was found in homozygous Z males. Males heterozygous for this autosomal factor exhibited similar neurological responses to both isomers of pheromone. Finally, response to the pheromone is controlled by two factors, a sex-linked gene on the Z chromosome and another on an autosome.[16] In species of Lepidoptea, sex is determined through the ZW sex-determination system where males are homozygous ZZ and females are heterozygous ZW.[17]

Males also produce sex pheromones that are structurally similar to those released by females. Composition of male pheromones is essential to female acceptance. The composition of male pheromones varies with age. Females prefer the pheromones of older males. Divergence of the pheromone composition can result in reproductive isolation and eventual speciation. This evolution is thought to take place in a concerted way between males and females within a population.[14]

During her adult life of 18 to 24 days, a female can lay a total of 400 to 600 eggs.[7] The female European corn borer moth first lays eggs in June. The eggs are laid on the underside of corn plant leaves near the midvein. Around 90% of the eggs are laid on the leaf just below the primary ear leaf, and an equal number of eggs are laid above and below this leaf, with a slight bias towards the lower leaves. The egg masses are all laid within five leaves of the central ear leaf.[18] Brood sizes range from 15 to 30 eggs and egg masses are about 6 mm in diameter.[19] The period of egg laying is about 14 days with an average of 20 to 50 eggs per day.[7]

The male European corn borer produces a spermatophore ejaculate that contains spermatozoa to fertilize the female and protein to nourish the female, a nuptial gift. The cost of producing a spermatophore is relatively low compared to the female investment in oviposition. Males mate an average of 3.8 times during their life. The average refractory period between mating cycles for the male is 1.6 days. With each successive mating, the volume of the spermatophore decreases. This decreased spermatophore volume is associated with a decrease in female fecundity and fertility. Females who mate with males that have already mated are less likely to deposit all of their eggs.[20]

Biological control agents of corn borers include the hymenopteran parasitoid of the genus Trichogramma, the fungus Beauveria bassiana and the protozoa Nosema pyrausta.

Bt corn, a variety of genetically modified corn, has had its genome modified to include a synthetic version of an insecticidal gene from the Bacillus thuringiensis kurstaki. As a result, the corn variety produces a protein that kills the larvae of Lepidoptera, the taxonomic order which includes the European corn borer.[22]

Immature corn shoots accumulate a powerful antibiotic substance, DIMBOA, that serves as a natural defense against a wide range of pests and is also responsible for the relative resistance of immature corn to the European corn borer.

When planting Bt corn, farmers must plant an area of refuge corn. A refuge area is an area of crops that do not contain the insecticidal genes. This refuge area is necessary is to prevent the European corn borer and other pests from developing resistance to the Bt gene. Insects who feed on the non-Bt crops will not develop resistance, but will continue to mate with any moths that survive after eating the genetically-modified corn. It is rare for an insect to survive after eating Bt corn, but when these resistant individuals mate with moths from the refuge area, the offspring they produce will still be susceptible to the toxin.[23] Studies on the dispersal of European corn borers found that planting refuge corn within a half-mile of Bt crops prevents resistance.[24]