mRNA (guanine-N7-)-methyltransferase

RNMT
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	3BGV, 3EPP, 5E9J, 5E9W, 5E8J
Identifiers
Aliases	RNMT, MET, RG7MT1, hCMT1c, CMT1, CMT1c, cm1p, hCMT1, hMet, MRNA-methyltransferase, RNA guanine-7 methyltransferase, N7-MTase
External IDs	OMIM: 603514 MGI: 1915147 HomoloGene: 2816 GeneCards: RNMT
Gene location (Human)
Chr.	Chromosome 18 (human)[1]
End	13,764,556 bp[1]
Gene location (Mouse)
Chr.	Chromosome 18 (mouse)[2]
End	68,457,923 bp[2]
RNA expression pattern
	Top expressed in
	amniotic fluid; ; middle temporal gyrus; ; Brodmann area 23; ; tendon; ; Achilles tendon; ; internal globus pallidus; ; Brodmann area 9; ; cingulate gyrus; ; prefrontal cortex; ; hypothalamus;
	Top expressed in
	secondary oocyte; ; morula; ; primitive streak; ; substantia nigra; ; blastocyst; ; ganglionic eminence; ; cerebellar cortex; ; medial ganglionic eminence; ; cumulus cell; ; facial motor nucleus;
	More reference expression data
	More reference expression data
Gene ontology
Molecular function	methyltransferase activity; transferase activity; mRNA (guanine-N7-)-methyltransferase activity; protein binding; RNA binding;
Cellular component	mRNA cap binding complex; receptor complex; nucleus; nucleoplasm; fibrillar center; mRNA cap methyltransferase complex;
Biological process	mRNA processing; methylation; transcription by RNA polymerase II; RNA 5'-cap (guanine-N7)-methylation; cellular response to leukemia inhibitory factor; 7-methylguanosine mRNA capping;
	Sources:Amigo / QuickGO
Orthologs
	8731
	67897
	ENSG00000101654
	ENSMUSG00000009535
	O43148
	Q9D0L8
	NM_001308263; NM_003799; NM_001378132; NM_001378134; NM_001378135
	NM_001170953; NM_026440; NM_001361002; NM_001361003
	NP_001295192; NP_003790; NP_001365061; NP_001365063; NP_001365064
	NP_001164424; NP_080716; NP_001347931; NP_001347932
	Wikidata
View/Edit Human	View/Edit Mouse

Search
PMC	articles
PubMed	articles
NCBI	proteins

mRNA (guanine-N7-)-methyltransferase
mRNA (guanine-N7-)-methyltransferase
Identifiers
EC no.	2.1.1.56
CAS no.	56941-25-4
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
PMC
Search
PMC	articles
PubMed	articles
NCBI	proteins

In enzymology, a mRNA (guanine-N7-)-methyltransferase also known as mRNA cap guanine-N7 methyltransferase is an enzyme that catalyzes the chemical reaction

S-adenosyl-L-methionine + G(5')pppR-RNA

\rightleftharpoons

S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA (mRNA containing an N⁷-methylguanine cap)

Thus, the two substrates of this enzyme are S-adenosyl methionine and G(5')pppR-RNA, whereas its two products are S-adenosylhomocysteine and m7G(5')pppR-RNA. This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases.

In humans, mRNA cap guanine-N7 methyltransferase is encoded by the RNMT gene.[5][6][7]

Nomenclature

The systematic name of this enzyme class is S-adenosyl-L-methionine:mRNA (guanine-N7-)-methyltransferase. Other names in common use include:

messenger ribonucleate guanine 7-methyltransferase,
guanine-7-methyltransferase,
messenger RNA guanine 7-methyltransferase, and
S-adenosyl-L-methionine:mRNA (guanine-7-N-)-methyltransferase.
cap MTase

References

GRCh38: Ensembl release 89: ENSG00000101654 - Ensembl, May 2017
GRCm38: Ensembl release 89: ENSMUSG00000009535 - Ensembl, May 2017
"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
Pillutla RC, Shimamoto A, Furuichi Y, Shatkin AJ (Jan 1999). "Human mRNA capping enzyme (RNGTT) and cap methyltransferase (RNMT) map to 6q16 and 18p11.22-p11.23, respectively". Genomics. 54 (2): 351–3. doi:10.1006/geno.1998.5604. PMID 9828141.
Pillutla RC, Yue Z, Maldonado E, Shatkin AJ (Sep 1998). "Recombinant human mRNA cap methyltransferase binds capping enzyme/RNA polymerase IIo complexes". J Biol Chem. 273 (34): 21443–6. doi:10.1074/jbc.273.34.21443. PMID 9705270.
"Entrez Gene: RNMT RNA (guanine-7-) methyltransferase".

Ensinger MJ, Martin SA, Paoletti E, Moss B (1975). "Modification of the 5'-terminus of mRNA by soluble guanylyl and methyl transferases from vaccinia virus". Proc. Natl. Acad. Sci. U.S.A. 72 (7): 2525–9. Bibcode:1975PNAS...72.2525E. doi:10.1073/pnas.72.7.2525. PMC 432801. PMID 1058472.
Groner Y, Gilboa E, Aviv H (1978). "Methylation and capping of RNA polymerase II primary transcripts by HeLa nuclear homogenates". Biochemistry. 17 (6): 977–82. doi:10.1021/bi00599a005. PMID 629955.
Martin SA, Moss B (1975). "Modification of RNA by mRNA guanylyltransferase and mRNA (guanine-7-)methyltransferase from vaccinia virions". J. Biol. Chem. 250 (24): 9330–5. doi:10.1016/S0021-9258(19)40647-9. PMID 1194287.
Martin SA, Paoletti E, Moss B (1975). "Purification of mRNA guanylyltransferase and mRNA (guanine-7-) methyltransferase from vaccinia virions". J. Biol. Chem. 250 (24): 9322–9. doi:10.1016/S0021-9258(19)40646-7. PMID 1194286.
Langberg SR, Moss B (1981). "Post-transcriptional modifications of mRNA. Purification and characterization of cap I and cap II RNA (nucleoside-2'-)-methyltransferases from HeLa cells". J. Biol. Chem. 256 (19): 10054–60. doi:10.1016/S0021-9258(19)68740-5. PMID 7275966.
Ishikawa K, Nagase T, Nakajima D, et al. (1998). "Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 4 (5): 307–13. doi:10.1093/dnares/4.5.307. PMID 9455477.
Tsukamoto T, Shibagaki Y, Niikura Y, Mizumoto K (1998). "Cloning and characterization of three human cDNAs encoding mRNA (guanine-7-)-methyltransferase, an mRNA cap methylase". Biochem. Biophys. Res. Commun. 251 (1): 27–34. doi:10.1006/bbrc.1998.9402. PMID 9790902.
Saha N, Schwer B, Shuman S (1999). "Characterization of human, Schizosaccharomyces pombe, and Candida albicans mRNA cap methyltransferases and complete replacement of the yeast capping apparatus by mammalian enzymes". J. Biol. Chem. 274 (23): 16553–62. doi:10.1074/jbc.274.23.16553. PMID 10347220.
Yamada-Okabe T, Mio T, Kashima Y, et al. (2000). "The Candida albicans gene for mRNA 5-cap methyltransferase: identification of additional residues essential for catalysis". Microbiology. 145 (11): 3023–33. doi:10.1099/00221287-145-11-3023. PMID 10589710.
Wen Y, Shatkin AJ (2001). "Cap methyltransferase selective binding and methylation of GpppG-RNA are stimulated by importin-α". Genes Dev. 14 (23): 2944–9. doi:10.1101/gad.848200. PMC 317093. PMID 11114884.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Shafer B, Chu C, Shatkin AJ (2005). "Human mRNA Cap Methyltransferase: Alternative Nuclear Localization Signal Motifs Ensure Nuclear Localization Required for Viability". Mol. Cell. Biol. 25 (7): 2644–9. doi:10.1128/MCB.25.7.2644-2649.2005. PMC 1061643. PMID 15767670.

This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000101654 - Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000009535 - Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid9828141-5] Pillutla RC, Shimamoto A, Furuichi Y, Shatkin AJ (Jan 1999). "Human mRNA capping enzyme (RNGTT) and cap methyltransferase (RNMT) map to 6q16 and 18p11.22-p11.23, respectively". Genomics. 54 (2): 351–3. doi:10.1006/geno.1998.5604. PMID 9828141.

[pmid9705270-6] Pillutla RC, Yue Z, Maldonado E, Shatkin AJ (Sep 1998). "Recombinant human mRNA cap methyltransferase binds capping enzyme/RNA polymerase IIo complexes". J Biol Chem. 273 (34): 21443–6. doi:10.1074/jbc.273.34.21443. PMID 9705270.

[entrez-7] "Entrez Gene: RNMT RNA (guanine-7-) methyltransferase".

Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Diffusion-limited enzyme Coenzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor Enzyme activator
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list) EC7 Translocases (list)

mRNA (guanine-N7-)-methyltransferase

Nomenclature

See also

References

Further reading