Shewanella violacea

Shewanella violacea DSS12 (S. violacea) is a gram-negative bacterium located in marine sediment in the Ryukyu Trench at a depth of 5,110m.[1] The first description of this organism was published in 1998 by Japanese microbiologists Yuichi Nogi, Chiaki Kato, and Koki Horikoshi, who named the species after its violet[2] appearance when it is grown on Marine Agar 2216 Plates.[1]

Shewanella violacea
Scientific classification Edit this classification
Domain: Bacteria
Phylum: Pseudomonadota
Class: Gammaproteobacteria
Order: Alteromonadales
Family: Shewanellaceae
Genus: Shewanella
Species:
S. violacea
Binomial name
Shewanella violacea
Nogi, Kato & Horikoshi, 1999

Shewanella violacea is a motile rod-shaped bacterium with flagella.[3] It is a facultative anaerobic organism and considered an extremophile due to its optimal growing conditions at 8°C and 30 MPa.[4] Researchers are evaluating this species to better understand the specific mechanisms S. violacea uses in order to thrive in its unusually cold and high-pressure environment.

Taxonomy

Shewanella violacea is a member of the Shewanella genus. Recent evaluation of the Shewanella phylogeny has led to a division of this genus into two categories: Group 1 and Group 2. These categories were created from an evaluation of the 16S rRNA sequences as well as a comparison of membrane lipid compositions. Group 1 Shewanella species are mostly extremophiles while Group 2 Shewanella species are mostly mesophiles.[4] S. violacea is a member of Group 1 Shewanella due to specific genetic adaptations that have enabled the bacteria to thrive in extremely low temperatures and high pressures. Specifically, Group 1 species contain a notably higher percentage of polyunsaturated fatty acids integrated in their membranes.[4]

Location

Samples of S. violacea have been collected using the SHINKAI 6500 System, a crewed submersible operated by the Japan Marine Science and Technology Center. Samples have been collected from the Ryukyu Trench at a depth of 5,110 m. The bacteria are found in the topmost layer of the sediment in this marine environment.[1]

Structure and metabolism

Genome

The complete genome of S. violacea was successfully sequenced in 2010 using the Sanger method. S. violacea contains 4,962,103 base pairs. It has 4,346 protein genes and 169 RNA genes. The bacterium contains a single chromosome and no known plasmids. The G+C content is 44.7%.[5] The complete genome is accessible online as published by the National Center for Biotechnology Information (see external links).

Membrane composition

Shewanella violacea has an abnormally high percent of polyunsaturated fatty acids (PUFA) integrated into its phospholipids. In Shewanella violacea 14% of its fatty acids are eicosapentaenoic acids (EPA) which are a specific type of polyunsaturated fatty acid also known as 20:5ω3. Increased PUFA concentrations decrease the membrane fluidity and help the bacterium thrive in the cold temperatures.[4] The exact function of the unusual lipid composition found in S. violacea and other members of Group 1 Shewanella species is not yet fully understood. Nevertheless, high EPA levels in S. violacea have been correlated with greater rates of cell division in high pressures as well as in low temperatures.[6][7][8]

Ideal growth conditions

Shewanella violacea is an obligate psychrophile (cryophile). Its optimum growth temperature is 8°C. It is not able to grow or reproduce at 30°C. S. violacea is a facultative piezophile (barophile) which means that it is able to thrive in high pressure conditions. S. violacea is able to grow in pressure conditions ranging from 0.1 to 70 MPa (3). Its ideal pressure is 30 MPa.[4]

Maintenance of respiratory system at high pressures

Unlike many other Shewanella species, S. violacea has very few terminal reductases for anaerobic respiration, c-type cytocromes and no Fe(III) reduction outer membrane proteins involved in respiration.[5] A pressure related operon is believed to play an integral role in the regulation of the respiratory system in S. violacea.[9] Specifically, researchers are evaluating the significance of pressure regulated cytochromes. Cytochromes are hemeproteins involved in the generation of ATP via electron transport. S. violacea contains three main types of cytochromes. The first, CA is expressed at all viable pressures. The second, CB, is only expressed at low pressures. The third, a d-type cytochrome is expressed only when the cells are grown under high pressure. The d-type cytochrome in S. violacea is thus a critical means for the respiratory system to remain active at high pressures.[3]

References
  1. Nogi, Y., C. Kato, and K. Horikoshi. "Taxonomic studies of deep-sea barophilic Shewanella strains and description of Shewanella violacea sp. nov." Archives of Microbiology 170(5) (1998): 331–38. Print.
  2. H. Kobayashi, Y. Nogi, and K. Horikoshi. "New violet 3,3'-bipyridyl pigment purified from deep-sea microorganism Shewanella violacea DSS12" Extremophiles, 11 (2007): 245–250. Print.
  3. Chikuma, Sayaka, Ryota Kasahara, Chiaki Kato, and Hideyuki Tamegai. "Bacterial adaptation to high pressure: a respiratory system in the deep-sea bacterium Shewanella violacea DSS12." FEMS Microbiology Letters 267(1) (2007): 108–12. Print.
  4. Kato, Chiaki, and Yuichi Nogi. "Correlation between phylogenetic structure and function: examples from deep-sea Shewanella." FEMS Microbiology Ecology 35(3) (2001): 223–30. Print.
  5. Aono, Eiji et al. "Complete genome sequence and comparative analysis of Shewanella violacea, a psychrophilic and piezophilic bacterium from deep sea floor sediments." Molecular BioSystems 6 (2010): 1216–226. Print.
  6. Kawamoto, Jun et al. "Favourable effects of eicosapentaenoic acid on the late step of the cell division in a piezophilic bacterium, Shewanella violacea DSS12, at high-hydrostatic pressures." Environmental Microbiology 13(8) (2011): 2293-298. Print.
  7. Tamegai, H. "Piezotolerance of the respiratory terminal oxidase activity of the piezophilic Shewanella violacea DSS12 as compared to non-piezophilic species." Biosci. Biotechnol. Biochem. 75(5) (2011): 919–24. Print
  8. Kawamoto, J., T. Kurihara, K. Yamamoto, M. Nagayasu, Y. Tani, H. Mihara, M. Hosokawa, T. Baba, S. B. Sato, and N. Esaki. "Eicosapentaenoic acid plays a beneficial role in membrane organization and cell division of a cold-adapted bacterium, Shewanella livingstonensis Ac10." Journal of Bacteriology 191(2) (2009): 632–40. Print.
  9. Nakasone, Kaoru. "Piezoregulation of cytochrome bd biosynthesis in deep-sea bacterium Shewanella violacea DSS12." Research Reports of the School of Engineering 39 (2005): 11–14. Print.


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