Microbial inoculant

Microbial inoculants also known as soil inoculants or bioinoculants are agricultural amendments that use beneficial rhizosphericic or endophytic microbes to promote plant health. Many of the microbes involved form symbiotic relationships with the target crops where both parties benefit (mutualism). While microbial inoculants are applied to improve plant nutrition, they can also be used to promote plant growth by stimulating plant hormone production.[1][2] Although bacterial and fungal inoculants are common, inoculation with archaea to promote plant growth is being increasingly studied. [3]

Research into the benefits of inoculants in agriculture extends beyond their capacity as biofertilizers. Microbial inoculants can induce systemic acquired resistance (SAR) of crop species to several common crop diseases (provides resistance against pathogens). So far SAR has been demonstrated for powdery mildew (Blumeria graminis f. sp. hordei, Heitefuss, 2001), take-all (Gaeumannomyces graminis var. tritici, Khaosaad et al., 2007), leaf spot (Pseudomonas syringae, Ramos Solano et al., 2008) and root rot (Fusarium culmorum, Waller et al. 2005).

However, it is increasingly recognized that microbial inoculants often modify the soil microbial community (Mawarda et al., 2020).

Bacterial

Rhizobacterial inoculants

The rhizobacteria commonly applied as inoculants include nitrogen-fixers, phosphate-solubilisers and other root-associated beneficial bacteria which enhance the availability of the macronutrients nitrogen and phosphorus to the host plant. Such bacteria are commonly referred to as plant growth promoting rhizobacteria (PGPR).

Nitrogen-fixing bacteria

The most commonly applied rhizobacteria are Rhizobium and closely related genera. Rhizobium are nitrogen-fixing bacteria that form symbiotic associations within nodules on the roots of legumes. This increases host nitrogen nutrition and is important to the cultivation of soybeans, chickpeas and many other leguminous crops. For non-leguminous crops, Azospirillum has been demonstrated to be beneficial in some cases for nitrogen fixation and plant nutrition.[1]

For cereal crops, diazotrophic rhizobacteria have increased plant growth,[4] grain yield (Caballero-Mellado et al., 1992), nitrogen and phosphorus uptake,[4] and nitrogen (Caballero-Mellado et al., 1992), phosphorus (Caballero-Mellado et al., 1992; Belimov et al., 1995) and potassium content (Caballero-Mellado et al., 1992). Rhizobacteria live in root nodes, and are associated with legumes.

Phosphate-solubilising bacteria

To improve phosphorus nutrition, the use of phosphate-solubilising bacteria (PSB) such as Agrobacterium radiobacter has also received attention (Belimov et al., 1995a; 1995b; Singh & Kapoor, 1999). As the name suggests, PSB are free-living bacteria that break down inorganic soil phosphates to simpler forms that enable uptake by plants.

Fungal inoculants

Symbiotic relationships between fungi and plant roots is referred to as a Mycorrhiza association.[5] This symbiotic relationships is present in nearly all land plants and give both the plant and fungi advantages to survival.[5] The plant can give upwards of 5-30% of its energy production to the fungi in exchange for increasing the root absorptive area with hyphae which gives the plant access to nutrients it would otherwise not be able to attain.[5][6] The two most common mycorrhizae are arbuscular mycorrhizae and ectomycorrhizae. Ectomycorrhizae associations are most commonly found in woody-species, and have less implications for agricultural systems.[7]  

Arbuscular mycorrhiza
This diagram shows the beneficial symbiotic relationship between a plants roots and a fungus partner, which is referred to as a mycorrhiza association.[5] Plants can give upwards of 5-30% of their photosynthetic production to this relationship, represented by G, in exchange for enhanced nutrient uptake, via hyphae, which extend the plants root absorptive area, giving it access to nutrients it would otherwise not be able to attain, which is represented by N and P.[5]

Arbuscular mycorrhiza (AM) has received attention as a potential agriculture amendment for its ability to access and provide the host plant phosphorus.[7] Under a reduced fertilization greenhouse system that was inoculated with a mixture of AM fungi and rhizobacteria, tomato yields that were given from 100% fertility were attained at 70% fertility.[8] This 30% reduction in fertilizer application can aid in the reduction of nutrient pollution, and help prolong finite mineral resources such as phosphorus (Peak phosphorus). Other effects include increases in salinity tolerance,[9] drought tolerance,[10] and resistance to trace metal toxicity.[11]

Fungal partners

Fungal inoculation alone can benefit host plants. Inoculation paired with other amendments can further improve conditions. Arbuscular mycorrhizal inoculation combined with compost is a common household amendment for personal gardens, agriculture, and nurseries. It has been observed that this pairing can also promote microbial functions in soils that have been affected by mining.[12]

Certain fungal partners do best in specific ecotones or with certain crops. Arbuscular mycorrhizal inoculation paired with plant growth promoting bacteria resulted in a higher yield and quicker maturation in upland rice paddys.[13]

Maize growth improved after an amendment of arbuscular mycorrhizae and biochar. This amendment can also decrease cadmium uptake by crops.[14]

Inoculant usage

Fungal inoculants can be used with or without additional amendments in private gardens, homesteads, agricultural production, native nurseries, and land restoration projects.

Composite inoculants

The combination of strains of Plant Growth Promoting Rhizobacteria (PGPR) has been shown to benefit rice and barley.[15][16] The main benefit from dual inoculation is increased plant nutrient uptake from both soil and fertilizer.[15] Multiple strains of inoculant have also been demonstrated to increase total nitrogenase activity compared to single strains of inoculants, even when only one strain is diazotrophic.[15][17][18]

PGPR and arbuscular mycorrhizae in combination can be useful in increasing wheat growth in nutrient poor soil[19] and improving nitrogen-extraction from fertilised soils.[20]

See also

References
  1. Bashan, Yoav; Holguin, Gina (1997). "Azospirillum – plant relationships: Environmental and physiological advances (1990–1996)". Canadian Journal of Microbiology. 43 (2): 103–121. doi:10.1139/m97-015. S2CID 6840330.
  2. Sullivan, Preston (2001). Alternative Soil Amendments (PDF) (Report). Appropriate Technology Transfer for Rural Areas.
  3. Chow, Chanelle; Padda, Kiran Preet; Puri, Akshit; Chanway, Chris P. (2022-09-20). "An Archaic Approach to a Modern Issue: Endophytic Archaea for Sustainable Agriculture". Current Microbiology. 79 (11): 322. doi:10.1007/s00284-022-03016-y. ISSN 1432-0991. PMID 36125558. S2CID 252376815.
  4. Galal, Y. G. M., El-Ghandour, I. A., Osman, M. E. & Abdel Raouf, A. M. N. (2003), The effect of inoculation by mycorrhizae and rhizobium on the growth and yield of wheat in relation to nitrogen and phosphorus fertilization as assessed by 15n techniques, Symbiosis, 34(2), 171-183.
  5. Brady, Nyle C. (2010). Elements of the nature and properties of soils. Weil, Ray R. (Third ed.). Upper Saddle River, N.J. pp. 343–346. ISBN 9780135014332. OCLC 276340542.{{cite book}}: CS1 maint: location missing publisher (link)
  6. "Mycorrhiza | David Sylvia's Web Resources". sites.psu.edu. Retrieved 2019-10-24.
  7. Chapin, F. Stuart; Matson, Pamela A.; Vitousek, Peter M. (2011). Principles of Terrestrial Ecosystem Ecology. New York, NY: Springer New York. pp. 243–244. doi:10.1007/978-1-4419-9504-9. ISBN 9781441995032.
  8. Adesemoye, A. O.; Torbert, H. A.; Kloepper, J. W. (November 2009). "Plant Growth-Promoting Rhizobacteria Allow Reduced Application Rates of Chemical Fertilizers". Microbial Ecology. 58 (4): 921–929. doi:10.1007/s00248-009-9531-y. ISSN 0095-3628. PMID 19466478. S2CID 8789559.
  9. Hirrel, M.C. and Gerdemann, J.W., 1980. Improved Growth of Onion and Bell Pepper in Saline Soils by Two Vesicular-Arbuscular Mycorrhizal Fungi 1. Soil Science Society of America Journal, 44(3), pp.654-655.
  10. Ferrazzano, S. and Williamson, P. (2013). Benefits of mycorrhizal inoculation in reintroduction of endangered plant species under drought conditions. Journal of Arid Environments, 98, pp.123-125.
  11. Firmin, S., Labidi, S., Fontaine, J., Laruelle, F., Tisserant, B., Nsanganwimana, F., Pourrut, B., Dalpé, Y., Grandmougin, A., Douay, F., Shirali, P., Verdin, A. and Lounès-Hadj Sahraoui, A. (2015). Arbuscular mycorrhizal fungal inoculation protects Miscanthus×giganteus against trace element toxicity in a highly metal-contaminated site. Science of the Total Environment, 527-528, pp.91-99.
  12. Kohler, J., Caravaca, F., Azcón, R., Díaz, G. and Roldán, A. (2015). The combination of compost addition and arbuscular mycorrhizal inoculation produced positive and synergistic effects on the phytomanagement of a semiarid mine tailing. Science of the Total Environment, 514, pp.42-48.
  13. Diedhiou, A., Mbaye, F., Mbodj, D., Faye, M., Pignoly, S., Ndoye, I., Djaman, K., Gaye, S., Kane, A., Laplaze, L., Manneh, B. and Champion, A. (2016). Field Trials Reveal Ecotype-Specific Responses to Mycorrhizal Inoculation in Rice. PLOS ONE, 11(12), p.e0167014.
  14. Liu, L., Li, J., Yue, F., Yan, X., Wang, F., Bloszies, S. and Wang, Y. (2018). Effects of arbuscular mycorrhizal inoculation and biochar amendment on maize growth, cadmium uptake and soil cadmium speciation in Cd-contaminated soil. Chemosphere, 194, pp.495-503.
  15. Belimov, A. A., Kojemiakov, A. P. & Chuvarliyeva, C. V. (1995a) Interaction between barley and mixed cultures of nitrogen fixing and phosphate-solubilising bacteria. Plant and Soil, 173, 29-37.
  16. Kennedy, Ivan R. (2001). "Biofertilisers in action". Functional Plant Biology. 28 (9): 825. doi:10.1071/pp01169. ISSN 1445-4408.
  17. Khammas, K. M.; Kaiser, P. (August 1992). "Pectin decomposition and associated nitrogen fixation by mixed cultures of Azospirillum and Bacillus species". Canadian Journal of Microbiology. 38 (8): 794–797. doi:10.1139/m92-129. ISSN 0008-4166. PMID 1458371.
  18. Cacciari, Isabella; Lippi, Daniela; Ippoliti, Silvia; Pietrosanti, Tito; Pietrosanti, Walter (July 1989). "Response to oxygen of diazotrophic Azospirillum brasilense ? Arthrobacter giacomelloi mixed batch culture". Archives of Microbiology. 152 (2): 111–114. doi:10.1007/bf00456086. ISSN 0302-8933. S2CID 10850392.
  19. Singh, S. & Kapoor, K. K. (1999) Inoculation with phosphate-solubilising microorganisms and a vesicular-arbuscular mycorrhizal fungus improves dry matter yield and nutrient uptake by wheat grown in sandy soil. Biology and Fertility of Soils, 28, 139-144.
  20. Galal, Y. G. M., El-Ghandour, I. A., Osman, M. E. & Abdel Raouf, A. M. N. (2003), The effect of inoculation by mycorrhizae and rhizobium on the growth and yield of wheat in relation to nitrogen and phosphorus fertilization as assessed by 15n techniques, Symbiosis, 34(2), 171-183.

Bibliography
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  • Bashan, Y., Holguin, G. & E., D.-B. L. (2004) Azospirillum-plant relationships: physiological, molecular, agricultural, and environmental advances (1997-2003). Canadian Journal of Microbiology, 50, 521-577.
  • Belimov, A. A., Kunakova, A. M., Vasilyeva, N. D., Gruzdeva, E. V., Vorobiev, N. I., Kojemiakov, A. P., Khamova, O. F., Postavskaya, S. M. & Sokova, S. A. (1995b) Relationship between survival rates of associative nitrogen-fixers on roots and yield response of plants to inoculation. FEMS Microbiology Ecology, 17, 187-196.
  • Caballero-Mellado, J., Carcano-Montiel, M. G. & Mascarua-Esparza, M. A. (1992), Field inoculation of wheat (triticum aestivum) with azospirillum brasilense under temperate climate, Symbiosis, 13, 243-253.
  • Gutierrez Manero, F. J. (2008) Systemic disease protection elicited by plant growth promoting rhizobacteria strains: relationship between metabolic responses, systemic disease protection, and biotic elicitors. Phytopathology, 98 (4), 451-457.
  • Heitefuss, R. (2001) Defence reactions of plants to fungal pathogens: principles and perspectives, using powdery mildew on cereals as an example. Naturwissenschaften, 88, 273-283.
  • Khammas, K. M. & Kaiser, P. (1992) Pectin decomposition and associated nitrogen fixation by mixed cultures of Azospirillum and Bacillus species. Canadian Journal of Microbiology, 38, 794-797.
  • Khaosaad, T., Garcia-Garrido, J. M., Steinkellner, S. & Vierheilig, H. (2007) Take-all disease is systemically reduced in roots of mycorrhizal barley plants. Soil Biology and Biochemistry, 39, 727-734.
  • Lippi, D., Cacciari, I., Pietrosanti, T. & Pietrosanti, W. (1992) Interactions between Azospirillum and Arthrobacter in diazotrophic mixed culture. Symbiosis, 13, 107-114.
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  • Nguyen, T. H., Kennedy, I. R. & Roughley, R. J. (2002) The response of field-grown rice to inoculation with a multi-strain biofertiliser in the Hanoi district, Vietnam. IN I. R. Kennedy & A. T. M. A. Choudhury (Eds.) Biofertilisers in Action. Barton, ACT, Rural Indrustries Research & Development Corporation.
  • Rabie, G. H. & Almadini, A. M. (2005) Role of bioinoculants in development of salt-tolerance of Vicia faba plants under salinity stress. African Journal of Biotechnology, 4 (3), 210-222.
  • Ramos Solano, R., Barriuso Maicas, J., Pereyra De La Iglesia, M. T., Domenech, J. &
  • Sullivan, P. (2001) Alternative soil amendments. Appropriate Technology Transfer for Rural Areas, National Center for Appropriate Technology. https://web.archive.org/web/20081011174000/http://www.attra.ncat.org/attra-pub/PDF/altsoil.pdf
  • Waller, F., Achatz, B., Baltruschat, H., Fodor, J., Becker, K., Fischer, M., Heier, T., Huckelhoven, R., Neumann, C., Von Wettstein, D., Franken, P. & Kogel, K.-H. (2005) The endophytic fungus Piriformis indica reprograms barley to salt-stress tolerance, disease resistance, and higher yield. Proceedings of the National Academy of Sciences, 102 (38), 13386-13391.
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