Synchronous culture

A synchronous or synchronized culture is a microbiological culture or a cell culture that contains cells that are all in the same growth stage.[1][2]

As numerous factors influence the cell cycle (some of them stochastic) normal cultures have cells in all stages of the cell cycle. Obtaining a culture with a unified cell-cycle stage is useful for biological research where a particular stage in the cell cycle is desired (such as the culturing of parasitized cells[3]). Since cells are too small for certain research techniques, a synchronous culture can be treated as a single cell; the number of cells in the culture can be easily estimated, and quantitative experimental results can simply be divided in the number of cells to obtain values that apply to a single cell. Synchronous cultures have been extensively used to address questions regarding cell cycle and growth, and the effects of various factors on these.[4]

Methods

Synchronous cultures can be obtained in several ways:

  • External conditions can be changed, so as to arrest growth of all cells in the culture, and then changed again to resume growth. The newly growing cells are now all starting to grow at the same stage, and they are synchronized. For example, for photosynthetic cells, light can be eliminated for several hours and then re-introduced. Another method is to eliminate an essential nutrient from the growth medium and later re-introduce it.[5]
  • Cell growth can also be arrested using chemical growth inhibitors. After growth has completely stopped for all cells, the inhibitor can be removed from the culture and the cells then begin to grow synchronously.[6] Nocodazole, for example, has been used in biological research for synchronization, although some evidence suggests it may lack such ability to synchronize cells.[7]
  • Cells in different growth stages have different physical properties. Cells in a culture can thus be physically separated based on their density or size, for instance. This can be achieved using centrifugation (for density) or filtration (for size).
  • In the Helmstetter-Cummings technique, a bacterial culture is filtered through a membrane. Most bacteria pass through, but some remain bound to the membrane. Fresh medium is then applied to the membrane and the bound bacteria start to grow. Newborn bacteria that detach from the membrane are now all at the same stage of growth; they are collected in a flask that now harbors a synchronous culture.[8]

References
  1. LLOYD, D.; et al. (1975). "Synchronous Cultures of Micro-organisms : Large-scale Preparation by Continuous-flow Size Selection" (PDF). Journal of General Microbiology. 88 (1): 153–158. doi:10.1099/00221287-88-1-153. PMID 807677.
  2. Thornton, Maureen; Eward, Kathryn Leigh; Helmstetter, Charles E. (May 2002). "Production of Minimally Disturbed Synchronous Cultures of Hematopoietic Cells" (PDF). BioTechniques. 32 (5): 1098–1105. doi:10.2144/02325rr05. PMID 12019783.
  3. Radfar, Azar; et al. (2009). "Synchronous culture of Plasmodium falciparum at high parasitemia levels". Nature Protocols. 4 (12): 1828–1844. doi:10.1038/nprot.2009.198. PMID 20010926. S2CID 238620.
  4. HARVEY, J. D. (1972). "Synchronous Growth of Cells and the Generation Time Distribution". Journal of General Microbiology. 70 (1): 99–107. doi:10.1099/00221287-70-1-99. PMID 5041269.
  5. Maruyamaa, Yoichi; Yanagitab, Tomomichi (May 1956). "Physical methods for obtaining synchronous culture of Escherichia coli". Journal of Bacteriology. 71 (5): 542–546. doi:10.1128/jb.71.5.542-546.1956. PMC 357850. PMID 13331866.
  6. KJAERGAARD, L.; JOERGENSEN, B. B. (1979). "Simple Equipment for Establishing Continuous Synchronous Cultures of Microorganisms". Biotechnology and Bioengineering. 21 (1): 147–151. doi:10.1002/bit.260210111.
  7. Cooper, Stephen; et al. (2006). "Nocodazole does not synchronize cells: implications for cell-cycle control and whole-culture synchronization" (PDF). Cell Tissue Res. 324 (2): 237–242. doi:10.1007/s00441-005-0118-8. hdl:2027.42/47691. PMID 16432713. S2CID 206989644. Archived from the original (PDF) on 2017-11-14.
  8. Shehata, Talaat E.; Marr, Allen G. (September 1970). "Synchronous Growth of Enteric Bacteria". Journal of Bacteriology. 103 (3): 789–792. doi:10.1128/jb.103.3.789-792.1970. PMC 248159. PMID 4919993.
This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.