Tandem repeat locus

Variable number of tandem repeat locus (VNTR locus) is any DNA sequence that exist in multiple copies strung together in a variety of tandem lengths.[1] The number of repeat copies present at a locus can be visualized by means of a Multi-locus or Multiple Loci VNTR Analysis (MLVA). In short, oligonucleotide primers are developed for each specific tandem repeat locus, followed by PCR and agarose gel electrophoresis. When the length of the repeat and the size of the flanking regions is known, the number of repeats can be calculated. Analysis of multiple loci will result in a genotype.[2]

References

Vergnaud G, Pourcel C (26 July 2018). "Multiple locus variable number of tandem repeats analysis". Molecular Epidemiology of Microorganisms. Methods in Molecular Biology. Vol. 551. pp. 141–58. doi:10.1007/978-1-60327-999-4_12. ISBN 978-1-60327-998-7. PMID 19521873.
Pöltl R, Luckenbach C, Ritter H (July 1998). "The short tandem repeat locus D3S1359". Forensic Science International. 95 (2): 163–8. doi:10.1016/S0379-0738(98)00087-5. PMID 9722977.

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[1] Vergnaud G, Pourcel C (26 July 2018). "Multiple locus variable number of tandem repeats analysis". Molecular Epidemiology of Microorganisms. Methods in Molecular Biology. Vol. 551. pp. 141–58. doi:10.1007/978-1-60327-999-4_12. ISBN 978-1-60327-998-7. PMID 19521873.

[2] Pöltl R, Luckenbach C, Ritter H (July 1998). "The short tandem repeat locus D3S1359". Forensic Science International. 95 (2): 163–8. doi:10.1016/S0379-0738(98)00087-5. PMID 9722977.