Thiazole synthase
Thiazole synthase (EC 2.8.1.10, thiG (gene)) is an enzyme with systematic name 1-deoxy-D-xylulose 5-phosphate:thiol sulfurtransferase.[1][2][3][4][5][6] This enzyme catalyses the following chemical reaction
- 1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
| Thiazole synthase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 Thiazole synthase oktamer, Saccharomyces cerevisiae | |||||||||
| Identifiers | |||||||||
| EC no. | 2.8.1.10 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
H2S can provide the sulfur in vitro.
References
- Park JH, Dorrestein PC, Zhai H, Kinsland C, McLafferty FW, Begley TP (October 2003). "Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B1)". Biochemistry. 42 (42): 12430–8. doi:10.1021/bi034902z. PMID 14567704.
- Dorrestein PC, Zhai H, McLafferty FW, Begley TP (October 2004). "The biosynthesis of the thiazole phosphate moiety of thiamin: the sulfur transfer mediated by the sulfur carrier protein ThiS". Chemistry & Biology. 11 (10): 1373–81. doi:10.1016/j.chembiol.2004.08.009. PMID 15489164.
- Dorrestein PC, Zhai H, Taylor SV, McLafferty FW, Begley TP (March 2004). "The biosynthesis of the thiazole phosphate moiety of thiamin (vitamin B1): the early steps catalyzed by thiazole synthase". Journal of the American Chemical Society. 126 (10): 3091–6. doi:10.1021/ja039616p. PMID 15012138.
- Settembre EC, Dorrestein PC, Zhai H, Chatterjee A, McLafferty FW, Begley TP, Ealick SE (September 2004). "Thiamin biosynthesis in Bacillus subtilis: structure of the thiazole synthase/sulfur carrier protein complex". Biochemistry. 43 (37): 11647–57. doi:10.1021/bi0488911. PMID 15362849.
- Hazra A, Chatterjee A, Begley TP (March 2009). "Biosynthesis of the thiamin thiazole in Bacillus subtilis: identification of the product of the thiazole synthase-catalyzed reaction". Journal of the American Chemical Society. 131 (9): 3225–9. doi:10.1021/ja806752h. PMC 2765510. PMID 19216519.
- Hazra AB, Han Y, Chatterjee A, Zhang Y, Lai RY, Ealick SE, Begley TP (June 2011). "A missing enzyme in thiamin thiazole biosynthesis: identification of TenI as a thiazole tautomerase". Journal of the American Chemical Society. 133 (24): 9311–9. doi:10.1021/ja1110514. PMC 3116082. PMID 21534620.
External links
- Thiazole+synthase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
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