Tissue typing

Mapping of HLA loci on chromosome 6

During tissue typing, an individual's human leukocyte antigens (HLA) are identified.[1] HLA molecules are presented on the surface of cells and facilitate interactions between immune cells (such as dendritic cells and T cells) that lead to adaptive immune responses.[2] If HLA from the donor is recognized by the recipient's immune system as different from the recipient's own HLA, an immune response against the donor tissues can be triggered.[3] More specifically, HLA mismatches between organ donors and recipients can lead to the development of anti-HLA donor-specific antibodies (DSAs).[4] DSAs are strongly associated with the rejection of donor tissues in the recipient, and their presence is considered an indicator of antibody-mediated rejection.[5] When donor and recipient HLA are matched, donor tissues are significantly more likely to be accepted by the recipient's immune system.[3] During tissue typing, a number of HLA genes should be typed in both the donor and recipient, including HLA Class I A, B, and C genes, as well as HLA Class II DRB1, DRB3, DRB4, DRB5, DQA1, DQB1, DPA1, and DPB1 genes.[6] HLA typing is made more difficult by the fact that the HLA region is the most genetically variable region in the human genome.[7]

This diagram shows serological typing. In the top half of the diagram, the correct antibody for the HLA type of the cell was added, so complement activation occurred, leading to cell lysis. Cell lysis indicates that the antibody added matched the HLA type of the cell, so the HLA type of the cell is then known. In the bottom half of the diagram, an HLA antibody that did not match the cell's HLA type was added, so there was no complement activation, and no cell lysis occurred.

One of the first methods of tissue typing was through serological typing. In this technique, a donor's blood cells are HLA typed by mixing them with serum containing anti-HLA antibodies. If the antibodies recognize their epitope on the donor's HLA then complement activation occurs leads to cell lysis and death, allowing the cells to take up a dye (trypan blue). This allows for identification of the cells' HLA based indirectly on the specificity of the known antibodies in the serum. This method has been used widely since it is simple, quick, and low-cost; however, the huge variability in HLA alleles means that serum containing antibodies specific to the HLA of the cells being tested may not be available.[6][3] Serological typing does not give a clear picture of the HLA region and does not always result in successful HLA typing, so many laboratories have stopped using it in favor of more effective methods.[6][8]

Recently, other more effective approaches have emerged, including the use of polymerase chain reaction (PCR) based on sequence-specific primers (SSP) or sequence-specific oligonucleotide probes (SSOP).[3][6] However, SSP-PCR can be both time and resource consuming.[8] SSOP-PCR is better for HLA typing large numbers of individuals, for example, large numbers of donors for bone marrow registries.[8] RT-PCR is another approach to HLA typing that is fast and versatile, but it is expensive.[6]

Reference strand-mediated conformational analysis (RSCA) is yet another method used for HLA typing. In this method, an unknown HLA sample is mixed with a reference allele and run in a gel by electrophoresis.[8] RSCA is limited by the number of HLA reference alleles available since the HLA region is so diverse.[8]

Direct DNA sequencing is currently considered the best method of HLA typing, either by Sanger sequencing or next generation sequencing, though it can also be time-consuming and is one of the more expensive methods.[6][8] RNA sequencing can also be used, but many labs do not as RNA is unstable and prone to degradation.[8]

• Graft-versus-host disease
• Histocompatibility

• How Preimplantation tissue typing works, HFEA website
• Tissue+typing at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
• Understanding Tissue Typing