Van-Pires

Four ordinary teens were accidentally caught in the path of a falling meteor. The meteor transformed them into heroic robotic guardians to protect the night from the evil forces of Tracula (a reference to "Dracula") and the rest of the Van-Pires. Each Motor-Vater has the ability to fly and they also share the same weaknesses as their enemies; like the Van-Pires, the Motor-Vaters require gas to sustain themselves and must avoid the sun at all costs. To transform, each hero gets into the driver's seat of his or her Carfin (a portmanteau of "car" and "coffin") and shouts, "Mission Ignition!"

• Axle (Jason Hayes) – The leader of the Motor-Vaters. He takes his role seriously and does what it takes to keep the team together. However, Axle can be too serious with his responsibilities either causing self-doubt when something goes awry or alienating one of the other Motor-Vaters. In his Motor-Vater form, Axle's color scheme is yellow with red fire designs. These colors are the same as his Carfin, which resembles a Lincoln convertible.
• Snap (Garikayi Mutambirwa) – Snap is a very laid back person but is ready to jump into action when trouble rises. Unlike the other Motor-Vaters (who speak exclusively in car puns), he speaks exclusively in a mangled form of African American Vernacular English. His Motor-Vater form is blue with a purple trim. Snap's Carfin is a blue hippie van.
• Nuke (Marc Schwarz) – Nuke is the team's strongman and thrill seeker. He has mastered the art of weaponized flatulence. His Motor-Vater form is green and modeled after a tow truck, complete with hook and crane. Nuke's Carfin is also a green tow truck.
• Rev (Melissa Marsala) – Rev is a tomboy, but she gets down and dirty just like the rest of the guys. Sometimes, Rev is not afraid to show off her feminine side. She is the target of both Nuke and Tracula's romantic affections, but she ignores them both because of her special relationship with her mentor Van He'llsing. In her Motor-Vater form, she is a red and armed with a fire hose and axe. Her Carfin is a fire truck.

• Van He'll Sing (Unknown as the character is credited as playing himself) – The Motor-Vaters' friend and advisor. Van is an old hippie that runs Sunrise Salvage, a junkyard in which the gang works and hangs out. His name is derived from the famous Bram Stoker character Van Helsing.
• Gypsy – An old female gypsy cab with mufflers for arms. She shows up at random times and gives the Motor-Vaters advice in the form of riddles.
• Greaspot – The Motor-Vaters' pet. He is a neon-colored plastic tricycle with the behavioral instincts of a puppy. It is never explained why Greaspot does not thirst for fuel (though it is possible that, because he's a tricycle, he simply has no need for fuel) or why he does not turn to dust in the sun.

The titular villains of the series. Unlike the Motor-Vaters, they do not have human forms. Led by Tracula, they terrorize the night with a never-ending thirst for gasoline. Like vampires, Van-Pires feed off of vehicles and can sire other Van-Pires to accomplish their evil goals.

• Tracula (Jonathan Davis) – The leader of the Van-Pires, and the main villain of the series. Tracula is a purple monster truck that demands obedience from his minions.
• Cardaver – A rusty and decrepit hunchbacked Van-Pire modeled after a hearse.
• Ambula (Donna Daley) – A female ambulance with six arms modeled after the Bride of Frankenstein. She is armed with an assortment of surgical tools and anaesthesia. She has an Electra complex (with Tracula playing the role of Agamemnon and Rev playing the role of Clytemnestra).
• Automaniac – An ice cream truck with a clown motif. Automaniac appears goofy and harmless, but his arsenal of ice cream and balloon animals shows otherwise.
• Alucart (Andrew Francis) – Tracula's son, constructed out of leftover auto parts and meant to be Tracula's heir. He befriends the Motor-Vaters, who dub him Alucart because it is his father's name backwards (in the same way that "Alucard" is Dracula spelled backwards). He only appeared in one episode and is based on Frankenstein’s Monster.