Xenos vesparum

Adult males are free-living, flying insects, whose extremely short (<5 hours) adult lives are solely dedicated to finding a mate, which they are thought to locate by scent.[2] After locating a mate (who is protruding from the host wasp's abdomen), the male lands on the wasp's abdomen, holding on with its legs and wings, while avoiding the brushing of the wasp's hindlegs, which could potentially dislodge it. The male then inseminates the female by either spreading sperm around the female's genital opening, where it eventually reaches the haemocoel (body cavity), or by directly penetrating the female's cuticle (hypodermic insemination), injecting the sperm directly into the haemocoel.[3] The male then dies several minutes after mating. Their small size coupled with their extremely short lifespan has made male X. vesparum very difficult to study.

Males also develop very unusual eyes compared to other insects. The eyes consist of a very small number of ommatidia (around 65, but each eye can vary by 10-15), while most insect eyes have thousands per eye which are closely packed together.[2] The ommatidia are irregularly distributed across the eye and are well separated by cuticle. The function of these eyes is unknown, because mate finding, which is their only purpose as adults, seems to be done by scent and the structure of the eyes indicate that they are modified larval eyes.[2] Interestingly, they are externally quite similar to the eyes of phacopid trilobites.[4]

The forewings of these insects are modified into small, club-like organs called pseudohalteres.[5] These are to help the insect maintain equilibrium in flight, and function similarly to halteres found in Dipterans.

female

Female X. vesparum are markedly different from their male counterparts. They display a high degree of neoteny, and are permanent endoparasites of their hosts. They reside in the wasp's body cavity and never develop mouthparts, legs, eyes or wings, and their only form of genitalia is the ventral opening where males can inseminate them, as well as being the point of larval escape.[1] Females can often survive overwinter inside hibernating female wasps, which will emerge the following spring with underdeveloped ovaries, and will only be able to serve as vessels to spread the parasite's larvae as they are now effectively castrated.[6]

Polistes sp. parasitized by Xenos vesparum

Female worker wasps infected with X. vesparum appear to have their behaviour modified because they act as if they are future queens and cease working, abandoning the nest before the uninfected future foundresses and males do, and form aggregations outside of the nest. When the uninfected future queens leave the nest to go to these mating grounds, it provides an area where uninfected and infected wasps mix, and this is often where release of infective larvae occurs.[9] Because the parasite does not modify male wasp behaviour, it is a "dead end" for female X. vesparum as the male wasps do not join in these large aggregations and provide little opportunity for the parasite to receive a mate. Additionally, the male wasp dies before winter, eliminating the chance of the larvae spreading in hibernating aggregations.[10] However, if infected with a male parasite, the male wasp is not a "dead end" because the free living male parasite can emerge and fly to a suitable mate within the mating aggregations. This may explain the recorded preference X. vesparum larvae have for infecting female wasps.[1]

A trend noted in paper wasps infected by this parasite is that they appear to exhibit a strong preference to congregating on Campsis radicans (trumpet creeper) bushes. This indicates a likely case of coevolution between parasite and host because the wasps are manipulated to prefer trumpet creeper bushes which provide shelter and excellent nutrition for the wasp, increasing its chance of survival, and by extension, increasing the parasite's chances as well.[11] In addition, the preference towards a specific plant type allows a greater chance of the parasite spreading because many foraging wasps are congregated in one spot, providing a vector for released infective larvae to reach the nest and infect young wasps.

Parasitized female wasps feature undeveloped ovaries as well as lowered levels of juvenile hormones and they abandon the colony without performing any of their caste's duties, meaning the host is castrated and reproductively "dead", leading some to describe X. vesparum a parasitoid as it applies to female wasps.[10] The effects on the male wasp's reproductive health is less understood. It appears that, at least for light to moderate parasite loads, the reproductive health of parasitized male wasps is virtually unaffected, while the effects of highly parasitized individuals (>4 parasites) is unknown.[12]