ZNF184

Zinc finger protein 184, also known as ZNF184, is a protein that in humans is encoded by the ZNF184 gene[5] on chromosome 6. It was first identified by Goldwurm et al. in 1996.[6]

ZNF184
Identifiers
AliasesZNF184, kr-ZNF3, zinc finger protein 184
External IDsOMIM: 602277 MGI: 1922244 HomoloGene: 113602 GeneCards: ZNF184
Orthologs
SpeciesHumanMouse
Entrez

7738

193452

Ensembl

ENSG00000096654

ENSMUSG00000006720

UniProt

Q99676

Q7TSH9

RefSeq (mRNA)

NM_007149
NM_001318891
NM_001318892
NM_001318893
NM_001347832

NM_183014
NM_001360886

RefSeq (protein)

NP_001305820
NP_001305821
NP_001305822
NP_001334761
NP_009080

NP_898835
NP_001347815

Location (UCSC)Chr 6: 27.45 – 27.47 MbChr 13: 22.13 – 22.14 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

The National Center for Biotechnology Information (NCBI) Gene database entry[5] for ZNF184 identifies conserved domains KRAB_A (Krüppel associated box) near the N-terminus and Zn-finger (Zinc finger) at the C-terminus of the translated protein. The former is associated with transcription repression[7] and the latter with DNA binding (see Zinc finger).

Domains and Structure

The figure below is a reformatted and annotated conceptual translation display of ZNF184's Consensus CDS.[8] CCDS displays exons in alternating black and blue font, with red indicating a residue coded across a splice boundary.

ZNF184 has 19 zinc finger motifs at the end of its final and longest exon. The figure shows regularity among the fingers in this protein, including the 2 columns of green-highlighted Cysteine residues and the 2 columns of blue-highlighted His residues which are the reason this type of zinc finger is called C2H2. Light grey highlighted columns (one with all F; one with mostly L, and F substitutions) are highly conserved hydrophobic residues within the zinc finger motif. The other light grey highlighted column (mostly K, with a similar R substitution) is an example of fairly strong conservation in the coil sections connecting adjacent fingers.

Near the N-terminus is a KRAB_A domain followed by a KRAB_B domain. KRAB_A has a shorter α-Helix followed by a longer α-Helix. The KRAB_A motif in a zinc finger protein is known to bind with a KAP-1 protein (aka TRIM28) to accomplish a transcription repressor function, however a gene so regulated by ZNF184 has yet to be identified. The length-11 finger helices are indicated, as well as the overlapping 7-residue section in each finger which binds targeted DNA (if the finger is functioning).

Reformatted Protein

References

  1. GRCh38: Ensembl release 89: ENSG00000096654 - Ensembl, May 2017
  2. GRCm38: Ensembl release 89: ENSMUSG00000006720 - Ensembl, May 2017
  3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. "Entrez Gene: zinc finger protein 184".
  6. Goldwurm S, Menzies ML, Banyer JL, Powell LW, Jazwinska EC (1997-03-15). "Identification of a novel Krueppel-related zinc finger gene (ZNF184) mapping to 6p21.3". Genomics. 40 (3): 486–9. doi:10.1006/geno.1996.4583. PMID 9073517.
  7. Peng H, Begg GE, Harper SL, Friedman JR, Speicher DW, Rauscher III FJ (2000-03-30). "Biochemical Analysis of the Kruppel-associated Box (KRAB) Transcriptional Repression Domain". Journal of Biological Chemistry. 275 (24): 18000–10. doi:10.1074/jbc.M001499200. PMID 10748030.
  8. "Consensus CDS: zinc finger protein 184".

Further reading

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