Egg hatch assay

Egg hatch assay (EHA), also called an egg hatch test (EHT),[1] is a method used to determine a given parasite's resistance to extant drug therapy.[2][3]

Fresh eggs are incubated from the parasite of interest and serial dilutions of the drug of interest are applied. The percentage of eggs that hatch or die is determined at each concentration and a drug response curve may be plotted. The data can then be transformed and analysed to give further statistics such as an effective dose.

This technique is labour-intensive, expensive and can take some time, however an egg hatch assay will give an accurate and reliable result.[4]

References
  1. von Samson-Himmelstjerna, Georg; Coles, Gerald C.; Jackson, Frank; Bauer, Christian; Borgsteede, Fred; Cirak, Veli Y.; Demeler, Janina; Donnan, Alison; Dorny, Pierre (2009-05-12). "Standardization of the egg hatch test for the detection of benzimidazole resistance in parasitic nematodes". Parasitology Research. 105 (3): 825–834. doi:10.1007/s00436-009-1466-1. ISSN 0932-0113. PMID 19452165. S2CID 21286130.
  2. Robles-Pérez, D.; Martínez-Pérez, J.M.; Rojo-Vázquez, F.A.; Martínez-Valladares, M. (2014-06-16). "Development of an egg hatch assay for the detection of anthelmintic resistance to albendazole in Fasciola hepatica isolated from sheep". Veterinary Parasitology. 203 (1–2): 217–221. doi:10.1016/j.vetpar.2013.11.020. hdl:10261/99726. ISSN 0304-4017. PMID 24576602.
  3. Albonico, Marco; Wright, Victoria; Ramsan, Mahdi; Haji, Hamadi J.; Taylor, Martin; Savioli, Lorenzo; Bickle, Quentin (June 2005). "Development of the egg hatch assay for detection of anthelminthic resistance in human hookworms". International Journal for Parasitology. 35 (7): 803–811. doi:10.1016/j.ijpara.2005.02.016. ISSN 0020-7519. PMID 15885696.
  4. Sargison, Neil (2009-01-26). Sheep Flock Health: A Planned Approach. John Wiley & Sons. ISBN 9781444302608.

Further reading
  • Jackson, F.; Jackson, E.; Coop, R. L. (2001). "Egg hatch assay for determination of resistance of nematodes to benzimidazole anthelmintics". In Halton, David W; Behnke, Jerzy M; Marshall, Ian (eds.). Practical Exercises in Parasitology. pp. 313–20. doi:10.1017/CBO9781139173353.038. ISBN 978-1-139-17335-3.
  • Demeler, Janina; Kleinschmidt, Nina; Küttler, Ursula; Koopmann, Regine; Von Samson-Himmelstjerna, Georg (2012). "Evaluation of the Egg Hatch Assay and the Larval Migration Inhibition Assay to detect anthelmintic resistance in cattle parasitic nematodes on farms". Parasitology International. 61 (4): 614–8. doi:10.1016/j.parint.2012.06.003. PMID 22728255.


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