Optimal cutting temperature compound
Optimal cutting temperature compound (OCT compound) is used to embed tissue samples prior to frozen sectioning on a microtome-cryostat. This process is undertaken so as to mount slices (sections) of a sample onto slides for analysis.
Frozen section procedure: tissue embedded in optimal cutting temperature compound, mounted on a chuck in a cryostat and ready for section production
Components
As stated on the bottle:
- 10.24% polyvinyl alcohol
- 4.26% polyethylene glycol
- 85.5% non-reactive ingredients"[1]
See also
- Microtome
- Cryostat
References
- "OCT Compound, 27050, MSDS". Tedpella.com. Retrieved 2011-08-19.
Further reading
- Turbett, Gavin; Sellner, Loryn (1997). "The use of optimal cutting temperature compound can inhibit amplification by polymerase chain reaction". Diagnostic Molecular Pathology. 6 (5): 298–303. doi:10.1097/00019606-199710000-00009. PMID 9458390.
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