Pseudomonas denitrificans

Pseudomonas denitrificans
Scientific classification
Domain:	Bacteria
Phylum:	Pseudomonadota
Class:	Gammaproteobacteria
Order:	Pseudomonadales
Family:	Pseudomonadaceae
Genus:	Pseudomonas
Species:	P. denitrificans
Binomial name
	Pseudomonas denitrificans; Bergey, et al. 1961
Type strain
	ATCC 19244 ; CCEB 525 ; IAM 12023 ; NCIMB 1656 ; NCTC 1656 ; NRRL B-1028

Pseudomonas denitrificans is a Gram-negative aerobic bacterium that performs denitrification. It was first isolated from garden soil in Vienna, Austria. It overproduces cobalamin (vitamin B₁₂), which it uses for methionine synthesis[1] and it has been used for manufacture of the vitamin.[2] Scientists at Rhône-Poulenc Rorer took a genetically engineered strain of the bacteria, in which eight of the cob genes involved in the biosynthesis of the vitamin had been overexpressed, to establish the complete sequence of methylation and other steps in the cobalamin pathway.[3][4]

Based on 16S rRNA analysis, P. denitrificans has been placed in the P. pertucinogena group.[5]

References

Lago BD, and Demain AL (1969). "Alternate requirement for vitamin B₁₂ or methionine in mutants of Pseudomonas denitrificans, a vitamin B₁₂-producing bacterium". J Bacteriol. 99 (1): 347–9. PMC 250011. PMID 5802615.
Fang, H; Kang, J; Zhang, D (30 January 2017). "Microbial production of vitamin B₁₂: a review and future perspectives". Microbial Cell Factories. 16 (1): 15. doi:10.1186/s12934-017-0631-y. PMC 5282855. PMID 28137297.
Debussche, L.; Thibaut, D.; Cameron, B.; Crouzet, J.; Blanche, F. (1993). "Biosynthesis of the corrin macrocycle of coenzyme B₁₂ in Pseudomonas denitrificans". Journal of Bacteriology. 175 (22): 7430–7440. doi:10.1128/jb.175.22.7430-7440.1993. PMC 206888. PMID 8226690.
R. Caspi (2013-09-25). "Pathway: adenosylcobalamin biosynthesis II (aerobic)". MetaCyc Metabolic Pathway Database. Retrieved 2020-04-24.
Anzai, Y.; Kim, H.; Park, J. Y.; Wakabayashi, H.; Oyaizu, H. (2000). "Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence". International Journal of Systematic and Evolutionary Microbiology. 50 (4): 1563–1589. doi:10.1099/00207713-50-4-1563. PMID 10939664.

External links

Type strain of Pseudomonas denitrificans at BacDive - the Bacterial Diversity Metadatabase

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[1] Lago BD, and Demain AL (1969). "Alternate requirement for vitamin B₁₂ or methionine in mutants of Pseudomonas denitrificans, a vitamin B₁₂-producing bacterium". J Bacteriol. 99 (1): 347–9. PMC 250011. PMID 5802615.

[pmid28137297-2] Fang, H; Kang, J; Zhang, D (30 January 2017). "Microbial production of vitamin B₁₂: a review and future perspectives". Microbial Cell Factories. 16 (1): 15. doi:10.1186/s12934-017-0631-y. PMC 5282855. PMID 28137297.

[PMC206888-3] Debussche, L.; Thibaut, D.; Cameron, B.; Crouzet, J.; Blanche, F. (1993). "Biosynthesis of the corrin macrocycle of coenzyme B₁₂ in Pseudomonas denitrificans". Journal of Bacteriology. 175 (22): 7430–7440. doi:10.1128/jb.175.22.7430-7440.1993. PMC 206888. PMID 8226690.

[aerobic_pathway-4] R. Caspi (2013-09-25). "Pathway: adenosylcobalamin biosynthesis II (aerobic)". MetaCyc Metabolic Pathway Database. Retrieved 2020-04-24.

[5] Anzai, Y.; Kim, H.; Park, J. Y.; Wakabayashi, H.; Oyaizu, H. (2000). "Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence". International Journal of Systematic and Evolutionary Microbiology. 50 (4): 1563–1589. doi:10.1099/00207713-50-4-1563. PMID 10939664.